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Microspheres were formed above the cloud point at 70°C. Dylight-488 (green) labeled microspheres were incubated above the cloud point for 12 or 45 minutes. Dylight-549 (red) labeled microspheres were incubated above the cloud point for 16 minutes. Dylight-633 (blue) labeled microspheres were incubated above the cloud point for 11 or 26 minutes. (a) Combined channels, (b) blue only, (c) green only, (d) red only.
