Skip to main content
NCBI home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1991 May;87(5):1519–1525. doi: 10.1172/JCI115162

Generation and analysis of clonal IgM- and IgG-producing human B cell lines expressing an anti-DNA-associated idiotype.

A J Manheimer-Lory 1, A Davidson 1, D Watkins 1, N R Hannigan 1, B A Diamond 1
PMCID: PMC295231  PMID: 1708781

Abstract

This study describes a methodology for generating stable, cloned, EBV-transformed IgG- and IgM-producing human B cell lines. Using these lines we have characterized immunoglobulin V gene utilization in an anti-DNA-associated idiotypic system. The 31 anti-DNA-associated idiotype is encoded preferentially by the VK1 gene family, and, in all probability, reflects a germ line gene-encoded framework determinant. Analysis of these lines indicates that the DNA-binding antibodies produced by B cell lines from SLE patients may differ from DNA binding myeloma proteins and from natural autoantibodies.

Full text

PDF
1519

Images in this article

1521Image
on p.1521
1521Image
on p.1521
1522Image
on p.1522
1522Image
on p.1522
1523Image
on p.1523
1523Image
on p.1523

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cairns E., Block J., Bell D. A. Anti-DNA autoantibody-producing hybridomas of normal human lymphoid cell origin. J Clin Invest. 1984 Sep;74(3):880–887. doi: 10.1172/JCI111505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Carson D. A., Freimark B. D. Human lymphocyte hybridomas and monoclonal antibodies. Adv Immunol. 1986;38:275–311. doi: 10.1016/s0065-2776(08)60009-7. [DOI] [PubMed] [Google Scholar]
  3. Casali P., Inghirami G., Nakamura M., Davies T. F., Notkins A. L. Human monoclonals from antigen-specific selection of B lymphocytes and transformation by EBV. Science. 1986 Oct 24;234(4775):476–479. doi: 10.1126/science.3020687. [DOI] [PubMed] [Google Scholar]
  4. Chen P. P., Goñi F., Fong S., Jirik F., Vaughan J. H., Frangione B., Carson D. A. The majority of human monoclonal IgM rheumatoid factors express a "primary structure-dependent" cross-reactive idiotype. J Immunol. 1985 May;134(5):3281–3285. [PubMed] [Google Scholar]
  5. Davidson A., Manheimer-Lory A., Aranow C., Peterson R., Hannigan N., Diamond B. Molecular characterization of a somatically mutated anti-DNA antibody bearing two systemic lupus erythematosus-related idiotypes. J Clin Invest. 1990 May;85(5):1401–1409. doi: 10.1172/JCI114584. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Davidson A., Preud'homme J. L., Solomon A., Chang M. D., Beede S., Diamond B. Idiotypic analysis of myeloma proteins: anti-DNA activity of monoclonal immunoglobulins bearing an SLE idiotype is more common in IgG than IgM antibodies. J Immunol. 1987 Mar 1;138(5):1515–1518. [PubMed] [Google Scholar]
  7. Davidson A., Smith A., Katz J., Preud'Homme J. L., Solomon A., Diamond B. A cross-reactive idiotype on anti-DNA antibodies defines a H chain determinant present almost exclusively on IgG antibodies. J Immunol. 1989 Jul 1;143(1):174–180. [PubMed] [Google Scholar]
  8. Halpern R., Davidson A., Lazo A., Solomon G., Lahita R., Diamond B. Familial systemic lupus erythematosus. Presence of a cross-reactive idiotype in healthy family members. J Clin Invest. 1985 Aug;76(2):731–736. doi: 10.1172/JCI112028. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hoch S., Schur P. H., Schwaber J. Frequency of anti-DNA antibody producing cells from normals and patients with systemic lupus erythematosus. Clin Immunol Immunopathol. 1983 Apr;27(1):28–37. doi: 10.1016/0090-1229(83)90053-3. [DOI] [PubMed] [Google Scholar]
  10. Isenberg D. A., Dudeney C., Williams W., Addison I., Charles S., Clarke J., Todd-Pokropek A. Measurement of anti-DNA antibodies: a reappraisal using five different methods. Ann Rheum Dis. 1987 Jun;46(6):448–456. doi: 10.1136/ard.46.6.448. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Logtenberg T., Young F. M., Van Es J. H., Gmelig-Meyling F. H., Alt F. W. Autoantibodies encoded by the most Jh-proximal human immunoglobulin heavy chain variable region gene. J Exp Med. 1989 Oct 1;170(4):1347–1355. doi: 10.1084/jem.170.4.1347. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Meek K., Jeske D., Slaoui M., Leo O., Urbain J., Capra J. D. Complete amino acid sequence of heavy chain variable regions derived from two monoclonal anti-p-azophenylarsonate antibodies of BALB/c mice expressing the major cross-reactive idiotype of the A/J strain. J Exp Med. 1984 Oct 1;160(4):1070–1086. doi: 10.1084/jem.160.4.1070. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nakamura M., Burastero S. E., Ueki Y., Larrick J. W., Notkins A. L., Casali P. Probing the normal and autoimmune B cell repertoire with Epstein-Barr virus. Frequency of B cells producing monoreactive high affinity autoantibodies in patients with Hashimoto's disease and systemic lupus erythematosus. J Immunol. 1988 Dec 15;141(12):4165–4172. [PubMed] [Google Scholar]
  14. Ralph P., Prichard J., Cohn M. Reticulum cell sarcoma: an effector cell in antibody-dependent cell-mediated immunity. J Immunol. 1975 Feb;114(2 Pt 2):898–905. [PubMed] [Google Scholar]
  15. Rauch J., Massicotte H., Tannenbaum H. Hybridoma anti-DNA autoantibodies from patients with rheumatoid arthritis and systemic lupus erythematosus demonstrate similar nucleic acid binding characteristics. J Immunol. 1985 Jan;134(1):180–186. [PubMed] [Google Scholar]
  16. Schwarzbaum S., Diamond B. The J744.2 cell line presents antigen in an I region restricted manner. J Immunol. 1983 Aug;131(2):674–677. [PubMed] [Google Scholar]
  17. Shoenfeld Y., Rauch J., Massicotte H., Datta S. K., André-Schwartz J., Stollar B. D., Schwartz R. S. Polyspecificity of monoclonal lupus autoantibodies produced by human-human hybridomas. N Engl J Med. 1983 Feb 24;308(8):414–420. doi: 10.1056/NEJM198302243080802. [DOI] [PubMed] [Google Scholar]
  18. Steffen D., Bird S., Rowe W. P., Weinberg R. A. Identification of DNA fragments carrying ecotropic proviruses of AKR mice. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4554–4558. doi: 10.1073/pnas.76.9.4554. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Trepicchio W., Jr, Barrett K. J. Eleven MRL-lpr/lpr anti-DNA autoantibodies are encoded by genes from four VH gene families: a potentially biased usage of VH genes. J Immunol. 1987 Apr 1;138(7):2323–2331. [PubMed] [Google Scholar]
  20. Zaghouani H., Bonilla F. A., Meek K., Bona C. Molecular basis for expression of the A48 regulatory idiotope on antibodies encoded by immunoglobulin variable-region genes from various families. Proc Natl Acad Sci U S A. 1989 Apr;86(7):2341–2345. doi: 10.1073/pnas.86.7.2341. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES