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. 1991 May;87(5):1559–1566. doi: 10.1172/JCI115169

Plasma lipid transfer protein as a determinant of the atherogenicity of monkey plasma lipoproteins.

E Quinet 1, A Tall 1, R Ramakrishnan 1, L Rudel 1
PMCID: PMC295238  PMID: 2022728

Abstract

This study was undertaken to determine potential tissue sources of plasma cholesteryl ester transfer protein (CETP), and to assess the influence of CETP on lipoprotein concentrations and atherosclerosis. In a group of 28 cynomolgus monkeys fed high fat, high cholesterol diets, plasma CETP concentration was strongly correlated with the abundance of CETP mRNA in liver and in adipose tissue, and with the output of CETP in liver perfusates. Plasma CETP concentration showed a strong inverse correlation with HDL cholesterol concentrations (r = -0.62, P less than 0.001) and a positive correlation with LDL cholesterol concentration (r = 0.54, P less than 0.005) and molecular weight (r = 0.57, P less than 0.001). The extent of coronary artery atherosclerosis was positively correlated with LDL cholesterol concentration and molecular weight, and with plasma CETP concentration. Thus, in monkeys fed an atherogenic diet, individual variation in CETP mRNA abundance in liver and adipose tissue probably plays a major role in the determination of plasma CETP levels. In plasma, CETP influences the distribution of cholesteryl esters between LDL and HDL, and CETP concentration appears to be a key determinant of the relative atherogenicity of the plasma lipoproteins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Auffray C., Rougeon F. Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA. Eur J Biochem. 1980 Jun;107(2):303–314. doi: 10.1111/j.1432-1033.1980.tb06030.x. [DOI] [PubMed] [Google Scholar]
  2. Babiak J., Lindgren F. T., Rudel L. L. Effects of saturated and polyunsaturated dietary fat on the concentrations of HDL subpopulations in African green monkeys. Arteriosclerosis. 1988 Jan-Feb;8(1):22–32. doi: 10.1161/01.atv.8.1.22. [DOI] [PubMed] [Google Scholar]
  3. Bisgaier C. L., Siebenkas M. V., Hesler C. B., Swenson T. L., Blum C. B., Marcel Y. L., Milne R. W., Glickman R. M., Tall A. R. Effect of a neutralizing monoclonal antibody to cholesteryl ester transfer protein on the redistribution of apolipoproteins A-IV and E among human lipoproteins. J Lipid Res. 1989 Jul;30(7):1025–1031. [PubMed] [Google Scholar]
  4. Brown M. L., Inazu A., Hesler C. B., Agellon L. B., Mann C., Whitlock M. E., Marcel Y. L., Milne R. W., Koizumi J., Mabuchi H. Molecular basis of lipid transfer protein deficiency in a family with increased high-density lipoproteins. Nature. 1989 Nov 23;342(6248):448–451. doi: 10.1038/342448a0. [DOI] [PubMed] [Google Scholar]
  5. Clarkson T. B., Bond M. G., Bullock B. C., Marzetta C. A. A study of atherosclerosis regression in Macaca mulatta. IV. Changes in coronary arteries from animals with atherosclerosis induced for 19 months and then regressed for 24 or 48 months at plasma cholesterol concentrations of 300 or 200 mg/dl. Exp Mol Pathol. 1981 Jun;34(3):345–368. doi: 10.1016/0014-4800(81)90052-6. [DOI] [PubMed] [Google Scholar]
  6. Gordon T., Castelli W. P., Hjortland M. C., Kannel W. B., Dawber T. R. High density lipoprotein as a protective factor against coronary heart disease. The Framingham Study. Am J Med. 1977 May;62(5):707–714. doi: 10.1016/0002-9343(77)90874-9. [DOI] [PubMed] [Google Scholar]
  7. Gordon T., Castelli W. P., Hjortland M. C., Kannel W. B., Dawber T. R. Predicting coronary heart disease in middle-aged and older persons. The Framington study. JAMA. 1977 Aug 8;238(6):497–499. [PubMed] [Google Scholar]
  8. Inazu A., Brown M. L., Hesler C. B., Agellon L. B., Koizumi J., Takata K., Maruhama Y., Mabuchi H., Tall A. R. Increased high-density lipoprotein levels caused by a common cholesteryl-ester transfer protein gene mutation. N Engl J Med. 1990 Nov 1;323(18):1234–1238. doi: 10.1056/NEJM199011013231803. [DOI] [PubMed] [Google Scholar]
  9. Koizumi J., Mabuchi H., Yoshimura A., Michishita I., Takeda M., Itoh H., Sakai Y., Sakai T., Ueda K., Takeda R. Deficiency of serum cholesteryl-ester transfer activity in patients with familial hyperalphalipoproteinaemia. Atherosclerosis. 1985 Dec;58(1-3):175–186. doi: 10.1016/0021-9150(85)90064-4. [DOI] [PubMed] [Google Scholar]
  10. Koritnik D. L., Rudel L. L. Measurement of apolipoprotein A-I concentration in nonhuman primate serum by enzyme-linked immunosorbent assay (ELISA). J Lipid Res. 1983 Dec;24(12):1639–1645. [PubMed] [Google Scholar]
  11. Kurasawa T., Yokoyama S., Miyake Y., Yamamura T., Yamamoto A. Rate of cholesteryl ester transfer between high and low density lipoproteins in human serum and a case with decreased transfer rate in association with hyperalphalipoproteinemia. J Biochem. 1985 Dec;98(6):1499–1508. doi: 10.1093/oxfordjournals.jbchem.a135418. [DOI] [PubMed] [Google Scholar]
  12. Kushwaha R. S., Rainwater D. L., Williams M. C., Getz G. S., McGill H. C., Jr Impaired plasma cholesteryl ester transfer with accumulation of larger high density lipoproteins in some families of baboons (Papio sp.). J Lipid Res. 1990 Jun;31(6):965–973. [PubMed] [Google Scholar]
  13. Marcel Y. L., McPherson R., Hogue M., Czarnecka H., Zawadzki Z., Weech P. K., Whitlock M. E., Tall A. R., Milne R. W. Distribution and concentration of cholesteryl ester transfer protein in plasma of normolipemic subjects. J Clin Invest. 1990 Jan;85(1):10–17. doi: 10.1172/JCI114397. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McGill H. C., Jr, McMahan C. A., Kruski A. W., Mott G. E. Relationship of lipoprotein cholesterol concentrations to experimental atherosclerosis in baboons. Arteriosclerosis. 1981 Jan-Feb;1(1):3–12. doi: 10.1161/01.atv.1.1.3. [DOI] [PubMed] [Google Scholar]
  15. Parks J. S., Johnson F. L., Wilson M. D., Rudel L. L. Effect of fish oil diet on hepatic lipid metabolism in nonhuman primates: lowering of secretion of hepatic triglyceride but not apoB. J Lipid Res. 1990 Mar;31(3):455–466. [PubMed] [Google Scholar]
  16. Quinet E. M., Agellon L. B., Kroon P. A., Marcel Y. L., Lee Y. C., Whitlock M. E., Tall A. R. Atherogenic diet increases cholesteryl ester transfer protein messenger RNA levels in rabbit liver. J Clin Invest. 1990 Feb;85(2):357–363. doi: 10.1172/JCI114446. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rudel L. L., Bond M. G., Bullock B. C. LDL heterogeneity and atherosclerosis in nonhuman primates. Ann N Y Acad Sci. 1985;454:248–253. doi: 10.1111/j.1749-6632.1985.tb11864.x. [DOI] [PubMed] [Google Scholar]
  18. Rudel L. L., Lee J. A., Morris M. D., Felts J. M. Characterization of plasma lipoproteins separated and purified by agarose-column chromatography. Biochem J. 1974 Apr;139(1):89–95. doi: 10.1042/bj1390089. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rudel L. L., Pitts L. L., 2nd, Nelson C. A. Characterization of plasma low density lipoproteins on nonhuman primates fed dietary cholesterol. J Lipid Res. 1977 Mar;18(2):211–222. [PubMed] [Google Scholar]
  20. Rudel L. L., Star R. J. Species, diet, and gender differences in plasma postheparin lipolytic activities in nonhuman primates. Relationships with plasma lipids and high density lipoproteins. Arteriosclerosis. 1990 May-Jun;10(3):350–357. doi: 10.1161/01.atv.10.3.350. [DOI] [PubMed] [Google Scholar]
  21. Sorci-Thomas M., Prack M. M., Dashti N., Johnson F., Rudel L. L., Williams D. L. Differential effects of dietary fat on the tissue-specific expression of the apolipoprotein A-I gene: relationship to plasma concentration of high density lipoproteins. J Lipid Res. 1989 Sep;30(9):1397–1403. [PubMed] [Google Scholar]
  22. Sorci-Thomas M., Wilson M. D., Johnson F. L., Williams D. L., Rudel L. L. Studies on the expression of genes encoding apolipoproteins B100 and B48 and the low density lipoprotein receptor in nonhuman primates. Comparison of dietary fat and cholesterol. J Biol Chem. 1989 May 25;264(15):9039–9045. [PubMed] [Google Scholar]
  23. Tall A. R. Plasma lipid transfer proteins. J Lipid Res. 1986 Apr;27(4):361–367. [PubMed] [Google Scholar]
  24. Whitlock M. E., Swenson T. L., Ramakrishnan R., Leonard M. T., Marcel Y. L., Milne R. W., Tall A. R. Monoclonal antibody inhibition of cholesteryl ester transfer protein activity in the rabbit. Effects on lipoprotein composition and high density lipoprotein cholesteryl ester metabolism. J Clin Invest. 1989 Jul;84(1):129–137. doi: 10.1172/JCI114132. [DOI] [PMC free article] [PubMed] [Google Scholar]

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