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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Bone. 2010 Aug 14;47(5):872–881. doi: 10.1016/j.bone.2010.08.007

Figure 4.

Figure 4

Fold change in Ptgs2 mRNA concentration in response to various levels of pulsatile fluid flow shear stress. MLO-Y4 osteocytic cells (A) and 2T3 osteoblastic cells (B) were subjected to fluid shear stresses of 2.0, 4.0, 8.0, 16.0, 24.0, and 32.0 dynes/cm2 with pulse heights of ±0.6 dynes/cm2 at a frequency of 0.5 Hz for 2 hours. Static controls were incubated for 2 hours in culture dishes. At the end of each fluid flow regimen, cells were either immediately lysed (0 hours post-flow, solid line) or returned to static culture in flow medium for 24 hours (24 hours post-flow, dashed line) followed by RNA extraction. Expression of Ptgs2 was quantitated by real-time PCR duplex reactions with Gapd as an endogenous control. ap < 0.01 compared to static controls. Data shown are mean ± sem. n=6.