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. 1991 Aug;88(2):379–384. doi: 10.1172/JCI115314

Interleukin 2 mediates stimulation of complement C3 biosynthesis in human proximal tubular epithelial cells.

R A Brooimans 1, A P Stegmann 1, W T van Dorp 1, A A van der Ark 1, F J van der Woude 1, L A van Es 1, M R Daha 1
PMCID: PMC295341  PMID: 1864952

Abstract

Previous reports have suggested the production of complement components C4, C2, and factor B by renal tissue. However, the cells involved in production of complement have not been identified. In this study metabolic labeling experiments demonstrated that human proximal tubular epithelial cells (PTEC) synthesize a 180-kD precursor of C3 that is secreted after proteolytic cleavage into a disulphide linked two-chain molecule as found in plasma. C3 present in culture supernatants of PTEC was functionally active, however, during the culture period there was a partial inactivation of the C3 molecule as assessed by hemolytic titration. Recombinant IL-2 enhances the rate of C3 synthesis in a dose-dependent manner reaching maximal stimulation at doses of 200-400 U/ml IL-2. Northern blot analysis demonstrated a 5.2-kb C3 mRNA species present in PTEC that was increased within 24 h of IL-2 treatment. IL-2-induced enhancement of C3 production by PTEC could be neutralized with specific antibodies to IL-2. This study demonstrates that C3 synthesis in PTEC is upregulated by IL-2, the major cytokine produced by activated T cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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