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. 1991 Aug;88(2):476–482. doi: 10.1172/JCI115328

Human anti-phosphorylcholine antibodies share idiotopes and are self-binding.

R Halpern 1, S V Kaveri 1, H Köhler 1
PMCID: PMC295366  PMID: 1864959

Abstract

We have previously shown that BALB/c antipneumococcal polysaccharide antibodies with phosphorylcholine (PC) specificity are self-binding, mediated by hypervariable sequence structure of the heavy chain. We extended the observation of self-binding anti-PC antibodies to naturally occurring human anti-PC antibodies. Anti-PC antibodies were purified from normal donor sera and shown to bind to monoclonal antiidiotypic anti-T15 antibodies originally raised against the murine T15 idiotype. These human antibodies are self-binding which is inhibitable by the PC hapten and the murine T15 (50-73)-derived Vh peptide. The anti-PC antibodies were further separated into id-positive and id-negative anti-PC antibodies. Only the T15 id-positive preparation was self-binding. These findings demonstrate an evolutionary, conserved biological property between mouse and man associated with a naturally occurring antibacterial antibody. This conserved biological and structural property may have been selected in evolution because it is part of an important immune defense mechanism against bacterial and other environmental pathogens.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Briles D. E., Forman C., Hudak S., Claflin J. L. Anti-phosphorylcholine antibodies of the T15 idiotype are optimally protective against Streptococcus pneumoniae. J Exp Med. 1982 Oct 1;156(4):1177–1185. doi: 10.1084/jem.156.4.1177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Briles D. E., Nahm M., Schroer K., Davie J., Baker P., Kearney J., Barletta R. Antiphosphocholine antibodies found in normal mouse serum are protective against intravenous infection with type 3 streptococcus pneumoniae. J Exp Med. 1981 Mar 1;153(3):694–705. doi: 10.1084/jem.153.3.694. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brown A. R., Crandall C. A. A phosphorylcholine idiotype related to TEPC 15 in mice infected with Ascaris suum. J Immunol. 1976 Apr;116(4):1105–1109. [PubMed] [Google Scholar]
  4. Chesebro B., Metzger H. Affinity labeling of a phosphorylcholine binding mouse myeloma protein. Biochemistry. 1972 Feb 29;11(5):766–771. doi: 10.1021/bi00755a014. [DOI] [PubMed] [Google Scholar]
  5. Gray B. M., Dillon H. C., Jr, Briles D. E. Epidemiological studies of Streptococcus pneumoniae in infants: development of antibody to phosphocholine. J Clin Microbiol. 1983 Nov;18(5):1102–1107. doi: 10.1128/jcm.18.5.1102-1107.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kang C. Y., Brunck T. K., Kieber-Emmons T., Blalock J. E., Kohler H. Inhibition of self-binding antibodies (autobodies) by a VH-derived peptide. Science. 1988 May 20;240(4855):1034–1036. doi: 10.1126/science.3368787. [DOI] [PubMed] [Google Scholar]
  7. Kang C. Y., Cheng H. L., Rudikoff S., Kohler H. Idiotypic self binding of a dominant germline idiotype (T15). Autobody activity is affected by antibody valency. J Exp Med. 1987 May 1;165(5):1332–1343. doi: 10.1084/jem.165.5.1332. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kang C. Y., Kohler H. Immunoglobulin with complementary paratope and idiotope. J Exp Med. 1986 Apr 1;163(4):787–796. doi: 10.1084/jem.163.4.787. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kaveri S. V., Kang C. Y., Köhler H. Natural mouse and human antibodies bind to a peptide derived from a germline VH chain. Evidence for evolutionary conserved self-binding locus. J Immunol. 1990 Dec 15;145(12):4207–4213. [PubMed] [Google Scholar]
  10. Kennedy R. C., Ionescu-Matiu I., Sanchez Y., Dreesman G. R. Detection of interspecies idiotypic cross-reactions associated with antibodies to hepatitis B surface antigen. Eur J Immunol. 1983 Mar;13(3):232–235. doi: 10.1002/eji.1830130310. [DOI] [PubMed] [Google Scholar]
  11. Lieberman R., Potter M., Mushinski E. B., Humphrey W., Jr, Rudikoff S. Genetics of a new IgVH (T15 idiotype) marker in the mouse regulating natural antibody to phosphorylcholine. J Exp Med. 1974 Apr 1;139(4):983–1001. doi: 10.1084/jem.139.4.983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. McNamara M. K., Ward R. E., Kohler H. Monoclonal idiotope vaccine against Streptococcus pneumoniae infection. Science. 1984 Dec 14;226(4680):1325–1326. doi: 10.1126/science.6505692. [DOI] [PubMed] [Google Scholar]
  13. Mond J. J., Lieberman R., Inman J. K., Mosier D. E., Paul W. E. Inability of mice with a defect in B-lymphocyte maturation to respond to phosphorycholine on immunogenic carriers. J Exp Med. 1977 Oct 1;146(4):1138–1142. doi: 10.1084/jem.146.4.1138. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Potter M., Leon M. A. Three IgA myeloma immunoglobulins from the BALB/ mouse: precipitation with pneumococcal C polysaccharide. Science. 1968 Oct 18;162(3851):369–371. doi: 10.1126/science.162.3851.369. [DOI] [PubMed] [Google Scholar]
  15. Raychaudhuri S., Kang C. Y., Kaveri S. V., Kieber-Emmons T., Köhler H. Tumor idiotype vaccines. VII. Analysis and correlation of structural, idiotypic, and biologic properties of protective and nonprotective Ab2. J Immunol. 1990 Jul 15;145(2):760–767. [PubMed] [Google Scholar]
  16. Riesen W. F. Idiotypic cross-reactivity of human and murine phosphorylcholine-binding immunoglobulins. Eur J Immunol. 1979 Jun;9(6):421–425. doi: 10.1002/eji.1830090602. [DOI] [PubMed] [Google Scholar]
  17. Sigal N. H., Chan M., Reale M. A., Moran T., Beilin Y., Schulman J. L., Bona C. The human and murine influenza-specific B cell repertoires share a common idiotope. J Immunol. 1987 Sep 15;139(6):1985–1990. [PubMed] [Google Scholar]
  18. Stein L. D., Sigal N. H. Heterogeneity of the human phosphocholine-specific B cell repertoire. J Immunol. 1984 Mar;132(3):1329–1335. [PubMed] [Google Scholar]
  19. Wittner M. K., Bach M. A., Köhler H. Immune response to phosphorylcholine. IX. Characterization of hybridoma anti-TEPC15 antibodies. J Immunol. 1982 Feb;128(2):595–599. [PubMed] [Google Scholar]

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