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. 1991 Aug;88(2):609–613. doi: 10.1172/JCI115345

Effects of body fat distribution on regional lipolysis in obesity.

M L Martin 1, M D Jensen 1
PMCID: PMC295396  PMID: 1864970

Abstract

To determine the contribution of the major body fat depots to systemic free fatty acid (FFA) availability, palmitate ([1-14C]-palmitate) release was measured from leg (lower body) and non-leg (upper body) fat in eight upper body obese (UB Ob), six lower body obese (LB Ob), and six nonobese (Non Ob) age-matched premenopausal women in the overnight postabsorptive state. Splanchnic palmitate release was determined in 16 of these subjects. Results: total palmitate release was greater in UB Ob (P less than 0.005) than LB Ob or Non Ob women (161 +/- 16 vs. 111 +/- 9 vs. 92 +/- 9 mumol/min, respectively). Despite increased leg fat mass in obese women, leg palmitate release was similar in each group. Therefore, leg fat palmitate release was greater in Non Ob women than LB Ob (P less than 0.01) or UB Ob (P = 0.06) women (3.7 +/- 0.3 vs. 2.4 +/- 0.2 vs. 2.7 +/- 0.2 mumol.kg fat-1.min-1, respectively). Upper body fat palmitate release was less (P less than 0.01) in LB Ob than Non Ob or UB Ob women (3.0 +/- 0.4 vs. 5.0 +/- 0.3 vs. 4.9 +/- 0.4 mumol.kg fat-1.min-1, respectively). Splanchnic palmitate release accounted for 20-32% of upper body fat palmitate release in each group (P = NS between groups). Leg fat palmitate release was significantly less than upper body fat palmitate release. We conclude that the major difference in resting FFA metabolism between UB Ob and LB Ob women is the ability of the later to down-regulate upper body fat lipolysis to maintain normal FFA availability.

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Selected References

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  1. Arner P., Kriegholm E., Engfeldt P., Bolinder J. Adrenergic regulation of lipolysis in situ at rest and during exercise. J Clin Invest. 1990 Mar;85(3):893–898. doi: 10.1172/JCI114516. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Basso L. V., Havel R. J. Hepatic metabolism of free fatty acids in normal and diabetic dogs. J Clin Invest. 1970 Mar;49(3):537–547. doi: 10.1172/JCI106264. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baumgartner R. N., Heymsfield S. B., Roche A. F., Bernardino M. Abdominal composition quantified by computed tomography. Am J Clin Nutr. 1988 Oct;48(4):936–945. doi: 10.1093/ajcn/48.4.936. [DOI] [PubMed] [Google Scholar]
  4. Bradley S. E., Ingelfinger F. J., Bradley G. P., Curry J. J. THE ESTIMATION OF HEPATIC BLOOD FLOW IN MAN. J Clin Invest. 1945 Nov;24(6):890–897. doi: 10.1172/JCI101676. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Després J. P., Nadeau A., Tremblay A., Ferland M., Moorjani S., Lupien P. J., Thériault G., Pinault S., Bouchard C. Role of deep abdominal fat in the association between regional adipose tissue distribution and glucose tolerance in obese women. Diabetes. 1989 Mar;38(3):304–309. doi: 10.2337/diab.38.3.304. [DOI] [PubMed] [Google Scholar]
  6. Evans D. J., Hoffmann R. G., Kalkhoff R. K., Kissebah A. H. Relationship of body fat topography to insulin sensitivity and metabolic profiles in premenopausal women. Metabolism. 1984 Jan;33(1):68–75. doi: 10.1016/0026-0495(84)90164-1. [DOI] [PubMed] [Google Scholar]
  7. Evans D. J., Murray R., Kissebah A. H. Relationship between skeletal muscle insulin resistance, insulin-mediated glucose disposal, and insulin binding. Effects of obesity and body fat topography. J Clin Invest. 1984 Oct;74(4):1515–1525. doi: 10.1172/JCI111565. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ferrannini E., Barrett E. J., Bevilacqua S., DeFronzo R. A. Effect of fatty acids on glucose production and utilization in man. J Clin Invest. 1983 Nov;72(5):1737–1747. doi: 10.1172/JCI111133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fujioka S., Matsuzawa Y., Tokunaga K., Tarui S. Contribution of intra-abdominal fat accumulation to the impairment of glucose and lipid metabolism in human obesity. Metabolism. 1987 Jan;36(1):54–59. doi: 10.1016/0026-0495(87)90063-1. [DOI] [PubMed] [Google Scholar]
  10. Garrow J. S. New approaches to body composition. Am J Clin Nutr. 1982 May;35(5 Suppl):1152–1158. doi: 10.1093/ajcn/35.5.1152. [DOI] [PubMed] [Google Scholar]
  11. Hennes M. M., Shrago E., Kissebah A. H. Receptor and postreceptor effects of free fatty acids (FFA) on hepatocyte insulin dynamics. Int J Obes. 1990 Oct;14(10):831–841. [PubMed] [Google Scholar]
  12. Herbert V., Lau K. S., Gottlieb C. W., Bleicher S. J. Coated charcoal immunoassay of insulin. J Clin Endocrinol Metab. 1965 Oct;25(10):1375–1384. doi: 10.1210/jcem-25-10-1375. [DOI] [PubMed] [Google Scholar]
  13. Jensen M. D., Braun J. S., Vetter R. J., Marsh H. M. Measurement of body potassium with a whole-body counter: relationship between lean body mass and resting energy expenditure. Mayo Clin Proc. 1988 Sep;63(9):864–868. doi: 10.1016/s0025-6196(12)62688-3. [DOI] [PubMed] [Google Scholar]
  14. Jensen M. D., Haymond M. W., Gerich J. E., Cryer P. E., Miles J. M. Lipolysis during fasting. Decreased suppression by insulin and increased stimulation by epinephrine. J Clin Invest. 1987 Jan;79(1):207–213. doi: 10.1172/JCI112785. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Jensen M. D., Haymond M. W., Rizza R. A., Cryer P. E., Miles J. M. Influence of body fat distribution on free fatty acid metabolism in obesity. J Clin Invest. 1989 Apr;83(4):1168–1173. doi: 10.1172/JCI113997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Jensen M. D. Regulation of forearm lipolysis in different types of obesity. In vivo evidence for adipocyte heterogeneity. J Clin Invest. 1991 Jan;87(1):187–193. doi: 10.1172/JCI114969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Jensen M. D., Rogers P. J., Ellman M. G., Miles J. M. Choice of infusion-sampling mode for tracer studies of free fatty acid metabolism. Am J Physiol. 1988 May;254(5 Pt 1):E562–E565. doi: 10.1152/ajpendo.1988.254.5.E562. [DOI] [PubMed] [Google Scholar]
  18. Jorfeldt L., Wahren J. Leg blood flow during exercise in man. Clin Sci. 1971 Nov;41(5):459–473. doi: 10.1042/cs0410459. [DOI] [PubMed] [Google Scholar]
  19. Kissebah A. H., Alfarsi S., Adams P. W., Wynn V. Role of insulin resistance in adipose tissue and liver in the pathogenesis of endogenous hypertriglyceridaemia in man. Diabetologia. 1976 Dec;12(6):563–571. doi: 10.1007/BF01220632. [DOI] [PubMed] [Google Scholar]
  20. Kissebah A. H., Vydelingum N., Murray R., Evans D. J., Hartz A. J., Kalkhoff R. K., Adams P. W. Relation of body fat distribution to metabolic complications of obesity. J Clin Endocrinol Metab. 1982 Feb;54(2):254–260. doi: 10.1210/jcem-54-2-254. [DOI] [PubMed] [Google Scholar]
  21. Krotkiewski M., Björntorp P., Sjöström L., Smith U. Impact of obesity on metabolism in men and women. Importance of regional adipose tissue distribution. J Clin Invest. 1983 Sep;72(3):1150–1162. doi: 10.1172/JCI111040. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Lafontan M., Dang-Tran L., Berlan M. Alpha-adrenergic antilipolytic effect of adrenaline in human fat cells of the thigh: comparison with adrenaline responsiveness of different fat deposits. Eur J Clin Invest. 1979 Aug;9(4):261–266. doi: 10.1111/j.1365-2362.1979.tb00883.x. [DOI] [PubMed] [Google Scholar]
  23. Mazess R. B., Barden H. S., Bisek J. P., Hanson J. Dual-energy x-ray absorptiometry for total-body and regional bone-mineral and soft-tissue composition. Am J Clin Nutr. 1990 Jun;51(6):1106–1112. doi: 10.1093/ajcn/51.6.1106. [DOI] [PubMed] [Google Scholar]
  24. Miles J. M., Ellman M. G., McClean K. L., Jensen M. D. Validation of a new method for determination of free fatty acid turnover. Am J Physiol. 1987 Mar;252(3 Pt 1):E431–E438. doi: 10.1152/ajpendo.1987.252.3.E431. [DOI] [PubMed] [Google Scholar]
  25. Peiris A. N., Mueller R. A., Smith G. A., Struve M. F., Kissebah A. H. Splanchnic insulin metabolism in obesity. Influence of body fat distribution. J Clin Invest. 1986 Dec;78(6):1648–1657. doi: 10.1172/JCI112758. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. RANDLE P. J., GARLAND P. B., HALES C. N., NEWSHOLME E. A. The glucose fatty-acid cycle. Its role in insulin sensitivity and the metabolic disturbances of diabetes mellitus. Lancet. 1963 Apr 13;1(7285):785–789. doi: 10.1016/s0140-6736(63)91500-9. [DOI] [PubMed] [Google Scholar]
  27. Rappaport P. L., Thiessen J. J. High-pressure liquid chromatographic analysis of indocyanine green. J Pharm Sci. 1982 Feb;71(2):157–161. doi: 10.1002/jps.2600710205. [DOI] [PubMed] [Google Scholar]
  28. Schlemmer A., Hassager C., Haarbo J., Christiansen C. Direct measurement of abdominal fat by dual photon absorptiometry. Int J Obes. 1990 Jul;14(7):603–611. [PubMed] [Google Scholar]
  29. Seidell J. C., Bakker C. J., van der Kooy K. Imaging techniques for measuring adipose-tissue distribution--a comparison between computed tomography and 1.5-T magnetic resonance. Am J Clin Nutr. 1990 Jun;51(6):953–957. doi: 10.1093/ajcn/51.6.953. [DOI] [PubMed] [Google Scholar]
  30. Svedberg J., Björntorp P., Smith U., Lönnroth P. Free-fatty acid inhibition of insulin binding, degradation, and action in isolated rat hepatocytes. Diabetes. 1990 May;39(5):570–574. doi: 10.2337/diab.39.5.570. [DOI] [PubMed] [Google Scholar]
  31. Wahrenberg H., Lönnqvist F., Arner P. Mechanisms underlying regional differences in lipolysis in human adipose tissue. J Clin Invest. 1989 Aug;84(2):458–467. doi: 10.1172/JCI114187. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Wang J., Heymsfield S. B., Aulet M., Thornton J. C., Pierson R. N., Jr Body fat from body density: underwater weighing vs. dual-photon absorptiometry. Am J Physiol. 1989 Jun;256(6 Pt 1):E829–E834. doi: 10.1152/ajpendo.1989.256.6.E829. [DOI] [PubMed] [Google Scholar]

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