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Replacement of CEN3 with the Pgal-CEN3 construct for the centromere reactivation system. The linearized plasmid pT389 (see Section 2.1.1) was integrated into the CEN3 locus through recombination at para-CEN3 regions. CEN3, para-CEN3 regions, GAL1-10 promoter, CYC1 transcription terminator, an array of tetOs, ampicillin-resistant gene, and URA3 gene are indicated.
