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. 1991 Sep;88(3):1043–1047. doi: 10.1172/JCI115365

Targeted and highly efficient gene transfer into CD4+ cells by a recombinant human immunodeficiency virus retroviral vector.

T Shimada 1, H Fujii 1, H Mitsuya 1, A W Nienhuis 1
PMCID: PMC295518  PMID: 1885765

Abstract

We have established a recombinant HIV gene transfer system based on transient expression of the HIV packaging functions and a recombinant vector genome in monkey kidney Cos cells. The recombinant HIV retroviral vector introduced the neoR gene into CD4+ cells with high efficiency, comparable to that achieved with the highest titer amphotropic murine recombinant retrovirus. Vector preparations were devoid of replication competent, infectious HIV. Gene transfer was dependent on CD4 expression, as shown by expression of the CD4 gene in HeLa cells, and could be inhibited by soluble CD4. This specific and efficient gene transfer system may be useful for development of gene therapy for which T cells are the desired targets.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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