In the Materials and Methods section two optimal primer sequences were switched by mistake with sub-optimal primer sequences. The correct sentences should be:
"We designed a universal-forward primer with an M13-tail added at the 5' end (M13BC-FW 5'-TGT AAA ACG ACG GCC AGT-HAA YCA YAA RGA YAT YGG -3'), similar to other primers previously used in other barcode approaches [e.g. 13]. Then, we searched for a reverse primer that allowed the specific amplification of vertebrate COI sequences while avoiding the co-amplification of invertebrate DNA (BCV-RV1 59-GCY CAN ACY ATN CCY ATR (T)(A)-3')."
Footnotes
Competing Interests: No competing interests declared.