Skip to main content
NCBI home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1991 Nov;88(5):1629–1635. doi: 10.1172/JCI115476

Differences in insulin action as a function of original anatomical site of newly differentiated adipocytes obtained in primary culture.

C Sztalryd 1, S Azhar 1, G M Reaven 1
PMCID: PMC295688  PMID: 1658046

Abstract

Stromal vascular cells were isolated from adipose tissue obtained from three different anatomical locations: epididymal (EPI), retroperitoneal (RP), and dorsal subcutaneous (SC), and allowed to differentiate in primary tissue culture. Cell number, protein concentration, glycerophosphate dehydrogenase, and lipoprotein lipase activity were similar in cells obtained from the EPI, RP, and SC regions, as were total insulin binding and the affinity of insulin for its receptor. However, both maximal insulin receptor tyrosine kinase activity and insulin-stimulated phosphorylation of the insulin receptor were significantly lower (P less than 0.05) in cells cultured from the SC region. In addition, newly differentiated adipocytes from the SC region were less sensitive to the ability of insulin to stimulate glucose uptake, and maximal insulin-stimulated glucose uptake by these cells was also significantly lower (P less than 0.05) when compared to cells obtained from the two other regions. Since these studies were performed on adipocyte precursor cells, allowed to differentiate to a similar degree in primary culture, the observed differences in insulin receptor phosphorylating activity, as well as the ability of insulin to stimulate glucose uptake appear to be intrinsic to adipose tissue from the three sites.

Full text

PDF
1629

Images in this article

1631Image
on p.1631
1631Image
on p.1631
1631Image
on p.1631
1631Image
on p.1631
1633Image
on p.1633
1633Image
on p.1633
1633Image
on p.1633

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arner P. Role of antilipolytic mechanisms in adipose tissue distribution and function in man. Acta Med Scand Suppl. 1988;723:147–152. doi: 10.1111/j.0954-6820.1987.tb05938.x. [DOI] [PubMed] [Google Scholar]
  2. Bertrand H. A., Stacy C., Masoro E. J., Yu B. P., Murata I., Maeda H. Plasticity of fat cell number. J Nutr. 1984 Jan;114(1):127–131. doi: 10.1093/jn/114.1.127. [DOI] [PubMed] [Google Scholar]
  3. Björntorp P. Abdominal obesity and the development of noninsulin-dependent diabetes mellitus. Diabetes Metab Rev. 1988 Sep;4(6):615–622. doi: 10.1002/dmr.5610040607. [DOI] [PubMed] [Google Scholar]
  4. Björntorp P., Karlsson M., Pertoft H., Pettersson P., Sjöström L., Smith U. Isolation and characterization of cells from rat adipose tissue developing into adipocytes. J Lipid Res. 1978 Mar;19(3):316–324. [PubMed] [Google Scholar]
  5. Bolinder J., Kager L., Ostman J., Arner P. Differences at the receptor and postreceptor levels between human omental and subcutaneous adipose tissue in the action of insulin on lipolysis. Diabetes. 1983 Feb;32(2):117–123. doi: 10.2337/diab.32.2.117. [DOI] [PubMed] [Google Scholar]
  6. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  7. DOLE V. P. A relation between non-esterified fatty acids in plasma and the metabolism of glucose. J Clin Invest. 1956 Feb;35(2):150–154. doi: 10.1172/JCI103259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Djian P., Roncari A. K., Hollenberg C. H. Influence of anatomic site and age on the replication and differentiation of rat adipocyte precursors in culture. J Clin Invest. 1983 Oct;72(4):1200–1208. doi: 10.1172/JCI111075. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Djian P., Roncari D. A., Hollenberg C. H. Adipocyte precursor clones vary in capacity for differentiation. Metabolism. 1985 Sep;34(9):880–883. doi: 10.1016/0026-0495(85)90114-3. [DOI] [PubMed] [Google Scholar]
  10. Donahue R. P., Abbott R. D., Bloom E., Reed D. M., Yano K. Central obesity and coronary heart disease in men. Lancet. 1987 Apr 11;1(8537):821–824. doi: 10.1016/s0140-6736(87)91605-9. [DOI] [PubMed] [Google Scholar]
  11. Eggstein M., Kreutz F. H. Eine neue Bestimmung der Neutralfette im Blutserum und Gewebe. I. Prinzip, Durchführung und Besprechung der Methode. Klin Wochenschr. 1966 Mar 1;44(5):262–267. doi: 10.1007/BF01747716. [DOI] [PubMed] [Google Scholar]
  12. Evans D. J., Hoffmann R. G., Kalkhoff R. K., Kissebah A. H. Relationship of androgenic activity to body fat topography, fat cell morphology, and metabolic aberrations in premenopausal women. J Clin Endocrinol Metab. 1983 Aug;57(2):304–310. doi: 10.1210/jcem-57-2-304. [DOI] [PubMed] [Google Scholar]
  13. Foley J. E., Kashiwagi A., Chang H., Huecksteadt T. P., Lillioja S., Verso M. A., Reaven G. Sex difference in insulin-stimulated glucose transport in rat and human adipocytes. Am J Physiol. 1984 Mar;246(3 Pt 1):E211–E215. doi: 10.1152/ajpendo.1984.246.3.E211. [DOI] [PubMed] [Google Scholar]
  14. Fried S. K., Lavau M., Pi-Sunyer F. X. Variations of glucose metabolism by fat cells from three adipose depots of the rat. Metabolism. 1982 Sep;31(9):876–883. doi: 10.1016/0026-0495(82)90176-7. [DOI] [PubMed] [Google Scholar]
  15. Gaben-Cogneville A. M., Poussin B., Chamblier M. C., Forgue-Lafitte M. E., Rosselin G. Development of insulin and epidermal growth factor receptors during the differentiation of rat preadipocytes in primary culture. Biochim Biophys Acta. 1988 Feb 22;968(2):231–238. doi: 10.1016/0167-4889(88)90012-2. [DOI] [PubMed] [Google Scholar]
  16. Glick J. M., Rothblat G. H. Effects of metabolic inhibitors on the synthesis and release of lipoprotein lipase in cultured cells derived from the stromal-vascular fraction of rat adipose tissue. Biochim Biophys Acta. 1980 Apr 18;618(1):163–172. doi: 10.1016/0005-2760(80)90063-6. [DOI] [PubMed] [Google Scholar]
  17. Godfrey K. Statistics in practice. Comparing the means of several groups. N Engl J Med. 1985 Dec 5;313(23):1450–1456. doi: 10.1056/NEJM198512053132305. [DOI] [PubMed] [Google Scholar]
  18. Hedo J. A., Harrison L. C., Roth J. Binding of insulin receptors to lectins: evidence for common carbohydrate determinants on several membrane receptors. Biochemistry. 1981 Jun 9;20(12):3385–3393. doi: 10.1021/bi00515a013. [DOI] [PubMed] [Google Scholar]
  19. Kissebah A. H., Vydelingum N., Murray R., Evans D. J., Hartz A. J., Kalkhoff R. K., Adams P. W. Relation of body fat distribution to metabolic complications of obesity. J Clin Endocrinol Metab. 1982 Feb;54(2):254–260. doi: 10.1210/jcem-54-2-254. [DOI] [PubMed] [Google Scholar]
  20. Kolterman O. G., Insel J., Saekow M., Olefsky J. M. Mechanisms of insulin resistance in human obesity: evidence for receptor and postreceptor defects. J Clin Invest. 1980 Jun;65(6):1272–1284. doi: 10.1172/JCI109790. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Krotkiewski M., Björntorp P. The effects of dexamethasone and starvation on body composition and regional adipose tissue cellularity in the rat. Acta Endocrinol (Copenh) 1975 Dec;80(4):667–675. doi: 10.1530/acta.0.0800667. [DOI] [PubMed] [Google Scholar]
  22. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  23. Lafontan M., Dang-Tran L., Berlan M. Alpha-adrenergic antilipolytic effect of adrenaline in human fat cells of the thigh: comparison with adrenaline responsiveness of different fat deposits. Eur J Clin Invest. 1979 Aug;9(4):261–266. doi: 10.1111/j.1365-2362.1979.tb00883.x. [DOI] [PubMed] [Google Scholar]
  24. Leibel R. L., Edens N. K., Fried S. K. Physiologic basis for the control of body fat distribution in humans. Annu Rev Nutr. 1989;9:417–443. doi: 10.1146/annurev.nu.09.070189.002221. [DOI] [PubMed] [Google Scholar]
  25. Lu Z. D., Piñeyro M. A., Kirkland J. L., Li Z. H., Gregerman R. I. Prostaglandin-sensitive adenylyl cyclase of cultured preadipocytes and mature adipocytes of the rat: probable role of Gi in determination of stimulatory or inhibitory action. J Cell Physiol. 1988 Jul;136(1):1–12. doi: 10.1002/jcp.1041360102. [DOI] [PubMed] [Google Scholar]
  26. Nilsson-Ehle P., Garfinkel A. S., Schotz M. C. Lipolytic enzymes and plasma lipoprotein metabolism. Annu Rev Biochem. 1980;49:667–693. doi: 10.1146/annurev.bi.49.070180.003315. [DOI] [PubMed] [Google Scholar]
  27. RABINOWITZ D., ZIERLER K. L. Forearm metabolism in obesity and its response to intra-arterial insulin. Characterization of insulin resistance and evidence for adaptive hyperinsulinism. J Clin Invest. 1962 Dec;41:2173–2181. doi: 10.1172/JCI104676. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. RODBELL M. METABOLISM OF ISOLATED FAT CELLS. I. EFFECTS OF HORMONES ON GLUCOSE METABOLISM AND LIPOLYSIS. J Biol Chem. 1964 Feb;239:375–380. [PubMed] [Google Scholar]
  29. Reaven G. M., Chang H., Hoffman B. B., Azhar S. Resistance to insulin-stimulated glucose uptake in adipocytes isolated from spontaneously hypertensive rats. Diabetes. 1989 Sep;38(9):1155–1160. doi: 10.2337/diab.38.9.1155. [DOI] [PubMed] [Google Scholar]
  30. Roskoski R., Jr Assays of protein kinase. Methods Enzymol. 1983;99:3–6. doi: 10.1016/0076-6879(83)99034-1. [DOI] [PubMed] [Google Scholar]
  31. Salans L. B., Cushman S. W., Weismann R. E. Studies of human adipose tissue. Adipose cell size and number in nonobese and obese patients. J Clin Invest. 1973 Apr;52(4):929–941. doi: 10.1172/JCI107258. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Wise L. S., Green H. Participation of one isozyme of cytosolic glycerophosphate dehydrogenase in the adipose conversion of 3T3 cells. J Biol Chem. 1979 Jan 25;254(2):273–275. [PubMed] [Google Scholar]
  33. Wise L. S., Green H. Studies of lipoprotein lipase during the adipose conversion of 3T3 cells. Cell. 1978 Feb;13(2):233–242. doi: 10.1016/0092-8674(78)90192-7. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES