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. 1991 Nov;88(5):1672–1679. doi: 10.1172/JCI115482

Evidence for persistent hepatitis C virus (HCV) infection in hemophiliacs.

J P Allain 1, S H Dailey 1, Y Laurian 1, D S Vallari 1, A Rafowicz 1, S M Desai 1, S G Devare 1
PMCID: PMC295699  PMID: 1939652

Abstract

Hepatitis C virus (HCV) is the major etiologic agent associated with non-A, non-B hepatitis. This study was designed to assess virologic and serologic markers in hemophiliacs exposed to non-heat-treated and/or virus-inactivated plasma derivatives. Serial bleeds from 48 hemophilic patients were analyzed for the presence of HCV viral RNA sequences as detected by polymerase chain reaction (PCR) and antibodies to structural (core) and nonstructural (C-100 and 33C) proteins by specific dot immunoblot assay. All patients exposed to non-heat-treated products, and four of six patients exposed only to virus inactivated products, had evidence of HCV infection. However, over the 5-yr study period, six exposed patients (13%) consistently lacked detectable anti-C-100 and seven (15%) lost this antibody. HCV viremia (PCR positive) was found in 91% of exposed patients, and was significantly more frequent in HIV seropositive hemophiliacs (P less than 0.05). Six patients had high antibody level to HCV and elevated ALT, but appeared to clear viremia. Four hemophiliacs were HCV seropositive but lacked detectable viremia. These data indicate that hemophiliacs remain persistently infected by HCV and that antibody to the core antigen of HCV is a reliable marker of this transfusion transmissible agent.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Antibodies to hepatitis C virus in haemophilia. Lancet. 1989 Sep 2;2(8662):560–561. [PubMed] [Google Scholar]
  2. Bolling T. J., Mandecki W. An Escherichia coli expression vector for high-level production of heterologous proteins in fusion with CMP-KDO synthetase. Biotechniques. 1990 May;8(5):488–492. [PubMed] [Google Scholar]
  3. Boudart D., Lucas J. C., Muller J. Y., Le Carrer D., Planchon B., Harousseau J. L. False-positive hepatitis C virus antibody tests in paraproteinaemia. Lancet. 1990 Jul 7;336(8706):63–63. doi: 10.1016/0140-6736(90)91585-x. [DOI] [PubMed] [Google Scholar]
  4. Brettler D. B., Alter H. J., Dienstag J. L., Forsberg A. D., Levine P. H. Prevalence of hepatitis C virus antibody in a cohort of hemophilia patients. Blood. 1990 Jul 1;76(1):254–256. [PubMed] [Google Scholar]
  5. Colombo M., Mannucci P. M., Carnelli V., Savidge G. F., Gazengel C., Schimpf K. Transmission of non-A, non-B hepatitis by heat-treated factor VIII concentrate. Lancet. 1985 Jul 6;2(8445):1–4. doi: 10.1016/s0140-6736(85)90055-8. [DOI] [PubMed] [Google Scholar]
  6. Coombs R. W., Collier A. C., Allain J. P., Nikora B., Leuther M., Gjerset G. F., Corey L. Plasma viremia in human immunodeficiency virus infection. N Engl J Med. 1989 Dec 14;321(24):1626–1631. doi: 10.1056/NEJM198912143212402. [DOI] [PubMed] [Google Scholar]
  7. Donohue J., Bailey M., Gray R., Holen J., Huang T. M., Keevan J., Mattimiro C., Putterman C., Stalder A., Defreese J. Enzyme immunoassay system for panel testing. Clin Chem. 1989 Sep;35(9):1874–1877. [PubMed] [Google Scholar]
  8. Esteban J. I., Esteban R., Viladomiu L., López-Talavera J. C., González A., Hernández J. M., Roget M., Vargas V., Genescà J., Buti M. Hepatitis C virus antibodies among risk groups in Spain. Lancet. 1989 Aug 5;2(8658):294–297. doi: 10.1016/s0140-6736(89)90485-6. [DOI] [PubMed] [Google Scholar]
  9. Fine J. M., Allain J. P., Rochu D., Marneux M. Occurrence of normal circulating proalbumin in a hemophilic A patient after acute hepatitis related to the delta virus. Clin Chem. 1986 Oct;32(10):1991–1993. [PubMed] [Google Scholar]
  10. Garson J. A., Preston F. E., Makris M., Tuke P., Ring C., Machin S. J., Tedder R. S. Detection by PCR of hepatitis C virus in factor VIII concentrates. Lancet. 1990 Jun 16;335(8703):1473–1473. doi: 10.1016/0140-6736(90)91510-h. [DOI] [PubMed] [Google Scholar]
  11. Garson J. A., Tuke P. W., Makris M., Briggs M., Machin S. J., Preston F. E., Tedder R. S. Demonstration of viraemia patterns in haemophiliacs treated with hepatitis-C-virus-contaminated factor VIII concentrates. Lancet. 1990 Oct 27;336(8722):1022–1025. doi: 10.1016/0140-6736(90)92487-3. [DOI] [PubMed] [Google Scholar]
  12. Horowitz M. S., Rooks C., Horowitz B., Hilgartner M. W. Virus safety of solvent/detergent-treated antihaemophilic factor concentrate. Lancet. 1988 Jul 23;2(8604):186–189. doi: 10.1016/s0140-6736(88)92288-x. [DOI] [PubMed] [Google Scholar]
  13. Kubo Y., Takeuchi K., Boonmar S., Katayama T., Choo Q. L., Kuo G., Weiner A. J., Bradley D. W., Houghton M., Saito I. A cDNA fragment of hepatitis C virus isolated from an implicated donor of post-transfusion non-A, non-B hepatitis in Japan. Nucleic Acids Res. 1989 Dec 25;17(24):10367–10372. doi: 10.1093/nar/17.24.10367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lai M. E., Farci P., Figus A., Balestrieri A., Arnone M., Vyas G. N. Hepatitis B virus DNA in the serum of Sardinian blood donors negative for the hepatitis B surface antigen. Blood. 1989 Jan;73(1):17–19. [PubMed] [Google Scholar]
  15. Laurence J. Molecular interactions among herpesviruses and human immunodeficiency viruses. J Infect Dis. 1990 Aug;162(2):338–346. doi: 10.1093/infdis/162.2.338. [DOI] [PubMed] [Google Scholar]
  16. Makris M., Preston F. E., Triger D. R., Underwood J. C., Choo Q. L., Kuo G., Houghton M. Hepatitis C antibody and chronic liver disease in haemophilia. Lancet. 1990 May 12;335(8698):1117–1119. doi: 10.1016/0140-6736(90)91124-s. [DOI] [PubMed] [Google Scholar]
  17. Mannucci P. M., Colombo M., Rodeghiero F. Non-A, non-B hepatitis after factor VIII concentrate treated by heating and chloroform. Lancet. 1985 Nov 2;2(8462):1013–1013. doi: 10.1016/s0140-6736(85)90560-4. [DOI] [PubMed] [Google Scholar]
  18. Mimms L., Vallari D., Ducharme L., Holland P., Kuramoto I. K., Zeldis J. Specificity of anti-HCV ELISA assessed by reactivity to three immunodominant HCV regions. Lancet. 1990 Dec 22;336(8730):1590–1591. doi: 10.1016/0140-6736(90)93377-2. [DOI] [PubMed] [Google Scholar]
  19. Preston F. E., Hay C. R., Dewar M. S., Greaves M., Triger D. R. Non-A, non-B hepatitis and heat-treated factor VIII concentrates. Lancet. 1985 Jul 27;2(8448):213–213. doi: 10.1016/s0140-6736(85)91525-9. [DOI] [PubMed] [Google Scholar]
  20. Rumi M. G., Colombo M., Gringeri A., Mannucci P. M. High prevalence of antibody to hepatitis C virus in multitransfused hemophiliacs with normal transaminase levels. Ann Intern Med. 1990 Mar 1;112(5):379–380. doi: 10.7326/0003-4819-112-5-379. [DOI] [PubMed] [Google Scholar]
  21. Schimpf K., Mannucci P. M., Kreutz W., Brackmann H. H., Auerswald G., Ciavarella N., Mösseler J., DeRosa V., Kraus B., Brueckmann C. Absence of hepatitis after treatment with a pasteurized factor VIII concentrate in patients with hemophilia and no previous transfusions. N Engl J Med. 1987 Apr 9;316(15):918–922. doi: 10.1056/NEJM198704093161505. [DOI] [PubMed] [Google Scholar]
  22. Simmonds P., Zhang L. Q., Watson H. G., Rebus S., Ferguson E. D., Balfe P., Leadbetter G. H., Yap P. L., Peutherer J. F., Ludlam C. A. Hepatitis C quantification and sequencing in blood products, haemophiliacs, and drug users. Lancet. 1990 Dec 15;336(8729):1469–1472. doi: 10.1016/0140-6736(90)93179-s. [DOI] [PubMed] [Google Scholar]
  23. Wang J. T., Wang T. H., Lin J. T., Sheu J. C., Lin S. M., Chen D. S. Hepatitis C virus RNA in saliva of patients with post-transfusion hepatitis C infection. Lancet. 1991 Jan 5;337(8732):48–48. doi: 10.1016/0140-6736(91)93365-g. [DOI] [PubMed] [Google Scholar]
  24. Weiner A. J., Kuo G., Bradley D. W., Bonino F., Saracco G., Lee C., Rosenblatt J., Choo Q. L., Houghton M. Detection of hepatitis C viral sequences in non-A, non-B hepatitis. Lancet. 1990 Jan 6;335(8680):1–3. doi: 10.1016/0140-6736(90)90134-q. [DOI] [PubMed] [Google Scholar]
  25. Weiner A. J., Truett M. A., Rosenblatt J., Han J., Quan S., Polito A. J., Kuo G., Choo Q. L., Houghton M., Agius C. HCV testing in low-risk population. Lancet. 1990 Sep 15;336(8716):695–695. doi: 10.1016/0140-6736(90)92194-m. [DOI] [PubMed] [Google Scholar]

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