FIGURE 5.
Identification of transcription factors involved in activation of the promoter. (A) Sequence of the core promoter [60 bp]2, with various REs probably involved in transcriptional activity shown in bold. The various mutations of the [60 bp] repeats are shaded in gray. (B) Identification, by mutagenesis, of transcription factors involved in the function of the promoter. The [60 bp] + 8 bp promoter was used for mutagenesis. Each reported luciferase activity value is normalized with respect to that of the [60 bp] + 8 bp promoter construct. (C) Identification, by mutagenesis, of transcription factors involved in the function of the promoter. The [60 bp]2 promoter was used for mutagenesis. Each reported luciferase activity value is normalized with respect to that of the [60 bp]2 promoter construct. White boxes indicate 60-bp repeats containing the various RE sites and gray boxes correspond to the first eight base pairs of the 60-bp repeat. Black boxes correspond to the p53 RE, and light gray boxes correspond to the SpiB RE. Dashed boxes indicate mutated sites.