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. Author manuscript; available in PMC: 2010 Oct 20.
Published in final edited form as: Breast. 2007 Oct 24;17(2):172–179. doi: 10.1016/j.breast.2007.08.009

Fig. 6.

Fig. 6

Enhancing effects of E2 on TfR regulation in ER+ MCF-7 (A) but not in ER− MCF-10A and MDA-MB-231 cells (B), and inhibitory effects of iron on TfR in all cells. ER+ MCF-7 cells were treated with E2 at 5 × 10−9 and 10 × 10−9 M in a 10-cm cell culture dish in 10% FBS α-MEM for 24 h, medium removed, then followed by exposure to iron at 25 and 50 µM in 0.1% FBS α-MEM for additional 24 h. ER− MCF-10A and MDA-MB-231 cells were treated with E2 at 5 × 10−9 M and/or iron at 25 µM in the same manner. Cells were collected and lysed using M-Per lysis buffer. Thirty microgram of protein samples were loaded to each well for TfR and β-tubulin Western blotting.