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ALP constructs used in this study. Diagram of pro-ALP protein with the TMD (black) and pro-sequence (dark grey) indicated. Putative N-glycosylation sites are shown by asterisks and scissors indicate the pro-sequence cleavage site. The Och1p TMD and cytosolic segments are shown as light grey boxes and the α-factor cleavable signal sequence as a black line. The triple HA epitope is represented as HA on the carboxyl terminus.
