Abstract
Intrauterine growth restriction (IUGR) is observed in conditions with limitations in uterine space (e.g., uterine anomalies and multifetal gestations). IUGR is associated with reduced fetal weight, organ growth, and a spectrum of adult-onset diseases. To examine the interaction of uterine anomalies and multifetal gestations, we developed a surgical uterine space restriction model with a unilateral uterine horn ligation before breeding (unilateral surgery). Placentas and fetuses were studied on Gestational Day (GD) 120 and GD 130 (term = 147 days). Unilateral surgery decreased placentome numbers in singleton and twin pregnancies (25% and 50%, respectively) but not unilateral triplets. Unilateral surgery decreased total placentome weight in twin pregnancies (decreased 24%). Fetuses categorized as uterine space restricted (unilateral twin and both groups of triplets) had 51% fewer placentomes per fetus and a 31% reduction in placentomal weight per fetus compared to the nonrestricted group (control singleton, unilateral singleton, and control twin). By GD 130, uterine space-restricted fetuses exhibited decreased weight, smaller crown-rump, abdominal girth, and thoracic girth as well as decreased fetal heart, kidney, liver, spleen, and thymus weights. Lung and brain weights were unaffected, demonstrating asymmetric IUGR. At GD 130, placental efficiency (fetal weight per total placentomal weight) was elevated in uterine space-restricted fetuses. However, fetal arterial creatinine, blood urea nitrogen, and cholesterol were elevated, suggesting insufficient placental clearance. Maternal-to-fetal glucose and triglycerides ratios were elevated in the uterine space-restricted pregnancies, suggesting placental nutrient transport insufficiency. This model allows for examination of interactive effects of uterine space restriction-induced IUGR on placental adaptation and fetal organ growth.
Keywords: cotyledon, fetal development, IUGR, placenta, placentome, pregnancy
Surgically induced uterine anomalies and multifetal gestation create uterine space restriction, resulting in fetal IUGR and altered placental development.
INTRODUCTION
Uterine anomalies are observed in 4.3% of women in the United States and are associated with both a reduction in fertility and an increased incidence of asymmetric intrauterine growth restriction (IUGR) [1]. The incidence of asymmetric IUGR is further compounded by multifetal pregnancies with a 65% to 85% greater instance of IUGR compared to singleton human pregnancies, resulting in dramatically increased morbidity to the fetus [2, 3]. Furthermore, both uterine anomalies (e.g., uterine bicornuate, unicornuate, or didelphys) and multifetal gestations are associated with decreased uterine space- and growth-restricted fetuses [4–12].
Uterine anomalies [4–6, 13, 14] and multifetal gestations [7–9] are two independent clinical factors associated with IUGR because of limiting uterine space, which in turn is associated with uteroplacental insufficiency. Current practices in assisted reproductive technologies have greatly improved conception and pregnancy rates in women with uterine anomalies [15]. Furthermore, these techniques also increase the incidence of multifetal pregnancies, further reducing uterine space. However, the interactive effects of these two conditions are not well characterized except in clinical case reports [1, 4–6, 14–18]. Additional biological and clinical significance are derived from studies demonstrating that IUGR is associated with a predisposition to a multitude of adult-onset diseases, including coronary heart disease, diabetes, dislipidaemia, and impaired neural development [12, 19, 20].
Thus, the aim of this study was to develop an ovine surgical model of uterine space restriction that investigates the interactive effects of uterine anomalies and multifetal gestations on fetal and placental growth. This model allows for the quantitative examination of IUGR relative to placental adaptation and fetal growth during development.
MATERIALS AND METHODS
Animals
Ewes of mixed Western breeds (n = 45) were obtained from the University of Wisconsin–Madison Arlington farm facility. Ewes were group housed throughout gestation and fed identical diets that consisted of a mixture of hay and corn silage that met the NRC requirement for all stages of gestation. Animal protocols were approved by University of Wisconsin–Madison Research Animal Care and Use Committee of the School of Medicine and Public Health and the College of Agriculture and Life Sciences.
Surgical Procedures
General surgical procedures [21–23] and the surgical model [24–28] were modified from those previously described. In brief, nonpregnant multiparous ewes were assigned to two treatment groups: unilateral (n = 21) and control (n = 24). Unilateral ewes were subjected to uterine surgical remodeling. Sheep were administered atropine (0.02 mg/kg, i.m.; Sigma Aldrich) and antibiotics (400 000 units of penicillin G benzathine and gentamicin sulfate; IVX Animal Health) and anesthetized with ketamine (16 mg/kg, i.m.; IVX Pharmaceuticals). The jugular vein was cannulated (Tygon tubing; 0.7-mm outer diameter and 0.4-mm inner diameter) for intravenous administration of ketamine (100 mg/ml) in 0.9% saline and 5% dextrose with supplemental sodium pentobarbital (50 mg/ml; Sigma Aldrich) as needed for additional anesthesia. A midventral laparotomy was performed to access the uterus. In the unilateral group, a single uterine horn was completely disconnected, including separation of all intercornual connections using an electrocautery, and one horn was double ligated with silk ligature and then transected (Fig. 1). Other than intercornual vascular connections, all the other uterine and ovarian vasculature was unaltered by this surgery. Furthermore, a subset of unilateral ewes had a single oviductal ligation (n = 8), ipsilateral to the horn ligated, whereas the remaining ewes (n = 13) did not. Similarly, control ewes either had a single oviductal ligation (n = 12) or had no surgery (n = 12). Single oviductal ligations were performed in an effort to achieve reduced fetal numbers. Statistical analyses demonstrated that oviductal ligation did not affect placental or fetal parameters (P > 0.05). After surgery, ewes were given flunixin meglumine (75 mg i.m.; IVX Pharmaceuticals) analgesia and given access to food and water ad libitum.
Reproductive Synchronization Protocol
At least 2 mo after surgery, a progesterone-controlled internal drug release (CIDR; 0.3 g; Pfizer) was placed in the vagina of these nonpregnant ewes. After at least 6 days, an i.m. injection of prostaglandin F2α (15 mg; Pfizer) was administered. Between Days 10 and 12, the CIDR was removed, and 500 IU equine chorionic gonadotropin (Sioux Biochemical Inc.) was injected i.m. Ewes were bred to a fertile ram, and pregnancy was confirmed by ultrasonography by gestational day (GD) 60.
Fetal Measurements, Tissue Collection, and Placentome Classification
Nonsurvival surgery was performed on either GD 120 (120.5 ± 0.4; n = 22) or GD 130 (129.8 ± 0.5; n = 23; term = 147 days) under a surgical plane of anesthesia with pentobarbital (50 mg/ml). Maternal arterial blood was collected via a catheter (Tygon tubing; 0.7-mm outer diameter and 0.4-mm inner diameter) inserted into the superficial saphenous branch of the femoral artery. Using a needle and heparinized syringe, umbilical vein and artery samples were simultaneously collected from each fetus prior to being removed from the uterus and euthanized. Blood was centrifuged (Beckman GPR Centrifuge) for 10 min at 3000 rpm (3600 × g) at 4°C, and plasma was aspirated and stored at −80°C. Plasma samples were further analyzed for clinical chemistry and nutritional components. Fetal growth was assessed by measuring fetal weight, crown-rump length, abdominal girth, thoracic girth, head length (tip of snout to crown of head), and head width (distance between ears). Fetal heart, kidney, liver, spleen, thymus, brain, and lung were collected and weighed. In addition, fetal body mass index (BMI) was calculated as fetal weight (kg)/crown-rump length (m2), and ponderal index (PI) was calculated as fetal weight (g)/crown-rump length (m3). A, B, C, and D placentomes were dissected from the uterus, classified according to the protocol of Vatnick and colleagues [29], individually counted, and weighed.
Fetuses were evaluated as either control or unilateral and by the number of fetuses as follows: control singletons (C-1; n = 9 fetuses), control twins (C-2, n = 14), control triplets (C-3, n = 21), control quadruplets (C-4, n = 4), unilateral singletons (U-1; n = 13), unilateral twins (U-2, n = 12), and unilateral triplets (U-3, n = 6). Placental data for GD 120 and GD 130 were combined because the number of placentomes and placental weight remain unchanged after GD 90 [30], and in this experiment total placentome number (P > 0.6) and total placentome weight (P > 0.3) were similar between GD groups.
Analysis of Placental Efficiency and Plasma Chemistry
As is traditional, placental efficiency was calculated as fetal weight (g)/placentome weight (g). Creatinine, blood urea nitrogen (BUN), and cholesterol were measured in the umbilical arterial plasma samples. Glucose and triglycerides were measured in the maternal arterial plasma and umbilical venous plasma samples. These data were obtained as part of a Chem 20 panel run in the Clinical Chemistry Laboratory at the Meriter Laboratories, Madison, WI (Cobas Integra 800; Roche Pharmaceuticals).
Statistical Analysis
Statistical analyses were performed using a one- or two-way analysis of variance followed by a Student-Newman-Keuls post hoc test using SigmaStat 1.0 software (Jandel Corporation). Data are presented as means ± SEM; P < 0.05 was considered significant.
RESULTS
Placental Changes with Uterine Space Restriction
A significant interaction between the factors “surgical uterine anomaly” and “multifetal gestation” was observed. Compared to singleton controls, twin and triplet control pregnancies had a 55% and a 32% increase in the total placentome number per ewe, respectively (Fig. 2A). Unilateral surgery intervention decreased the total number of placentomes in singleton and twin pregnancies (Fig. 2A). Singleton unilateral pregnancies had 25% fewer total placentomes per ewe compared to controls, and twin unilateral pregnancies had 50% fewer total placentomes per ewe. For triplet pregnancies, there was no difference in total placentome number per ewe between control and unilateral sheep, demonstrating overall placental adaptation. Compared to singletons, total placentome weight was increased by 68% and 120% in twin and triplet control pregnancies, respectively, but only by a 31% increase compared to twins (Fig. 2B). Unilateral surgery decreased total placentome weight by 24%, in twin pregnancies only, whereas singleton and triplet pregnancies had no difference in total placentome weight (Fig. 2B).
Based on the interactive effects of the uterine anomaly surgical intervention and the number of fetuses per pregnancy, we utilized the dependent placental factors, namely, the number of placentomes and the total placentomal weight per fetus, as indices of potential nutrient delivery to the fetus. We then categorized the fetuses as nonrestricted (control and unilateral singletons and control twins) and uterine space restricted (unilateral twins, control and unilateral triplets, and control quadruplets) (Fig. 3, A and B). Unilateral twins, all triplets and quadruplet pregnancies, had 51% fewer placentomes per fetus (54 ± 2 vs. 27 ± 2, respectively) and a 31% reduction in placentomal weight per fetus (515 ± 20 vs. 345 ± 16 g, respectively) compared to all singletons and control twins (Fig. 3, A and B; P < 0.0001).
Uterine space restriction altered the percentage of A and D placentomes. Compared to nonrestricted pregnancies, there were 42% fewer A and 78% more D placentomes in the uterine space-restricted group (Table 1). No difference was observed in the percentage of B and C placentomes between groups. Space restriction also showed a tendency (P = 0.08) to reduce total A placentome weight by 42% (also in Table 2). Further, there was a 65% increase in total C placentome weight and a 189% increase in total D weight in uterine space-restricted pregnancies. Total placentome weight was elevated by 40% in uterine space-restricted pregnancies. We also calculated mean placentome weight by dividing total placentome weight by the number of placentomes. Within each type, only D placentomes were heavier, increasing by 100%. Therefore, on average, each placentome was 41% larger in the uterine space-restricted group (Table 2).
TABLE 1.
TABLE 2.
Fetal Changes with Uterine Space Restriction
Compared to the nonrestricted group, uterine space-restricted fetuses were 22% lighter in weight at GD 130 and had a 6% and 9% shorter crown-rump length at GD 120 and GD 130, respectively (Fig. 4, A and B). In contrast, there was no difference in BMI or PI between nonrestricted and restricted fetuses at either GD (Fig. 4C). Fetal abdominal and thoracic girth were unchanged between groups at GD 120 (Fig. 5, A and B), but by GD 130, the uterine space-restricted fetuses had a 10% smaller abdominal and 9% smaller thoracic girth when compared with nonrestricted fetuses (Fig. 5, A and B). Brain sparing was observed in the uterine space-restricted fetuses, as fetal head length, head width, and brain weights were similar between groups at either GD 120 or GD 130 (Fig. 5, C and D; see also Table 3).
TABLE 3.
Uterine space restriction decreased heart, liver, and spleen weights at GD 120 and by GD 130 were 29%, 31%, and 34% lighter in restricted fetuses, respectively (Table 3). Kidney and thymus weights in the restricted group were not different at GD 120; however, by GD 130, they were 26% and 50% lighter, indicating a lack of continual growth. Fetal brain and lung weights were not different between groups at either GD (Table 3), demonstrating brain sparing and asymmetric IUGR. When weights were examined in proportion to fetal body weight (standardized fetal weight), there were no differences in heart, kidney, liver, and lung weights per fetal body weight between groups at either GD (data not shown). In contrast, compared to nonrestricted fetuses, uterine space-restricted spleen and thymus weights per fetal weight were not different at GD 120; however, by GD 130, they were 21% and 36% lighter (data not shown). Uterine space-restricted brain weights per fetal weight were 22% and 19% larger at GD 120 and GD 130, respectively (data not shown).
Placental Efficiency and Blood Chemistry Changes with Uterine Space Restriction
Classically calculated placental efficiency is the ratio of fetal weight (g) per placental weight (g). According to this definition, placentas showed similar efficiency at GD 120, and by this definition, uterine space-restricted placentas were 15% more efficient by GD 130 (Fig. 6A). We also evaluated fetal levels of creatinine, BUN, and cholesterol (Fig. 6, B–D). At GD 120, uterine space restriction did not alter umbilical artery plasma creatinine, BUN, or cholesterol levels. However, at GD 130, uterine space-restricted fetuses had 34% higher umbilical arterial creatinine, 15% higher BUN, and 67% higher cholesterol. The maternal artery-to-umbilical vein ratio of glucose and triglycerides (Fig. 6, E and F) were unchanged at GD 120, whereas the ratios were increased 32% and 73% in GD 130 space-restricted fetuses.
DISCUSSION
This study provides the first in-depth examination of the interactions between multifetal gestations and a surgically created uterine anomaly model on placental adaptations and IUGR, giving us the ability to quantify specific placental adaptations that function to modulate fetal growth and development. The placenta adapted by increasing the total number of placentomes formed, increasing individual placentomal weights through greater conversion from A to D morphological types, and increasing C and D total placentomal weights. Multifetal pregnancies with uterine anomaly induced fetal growth arrest and asymmetric IUGR once the ability of the placenta to adapt was surpassed. These fetal growth deficits were associated with alterations in functional placental efficiency and adaptations that are vitally important for maintaining a viable albeit compromised pregnancy.
Several earlier ovine models of uterine space reduction were developed by reducing the endometrial surface area and thus the potential placental attachment sites. These models have reduced the number of placentomes by either carunclectomy prior to breeding [31, 32], by hemihysterectomy, [33] or, in a more analogous fashion to the current model, by performing unilateral uterine horn ligations either before [34] or 5 days after breeding [35, 36]. In the unilateral ligation and hemihysterectomy models, placentome number was reduced by 14% [33], 23% [34], and 47% [36]. Although some of these studies had twin pregnancies [36], they did not stratify their multifetal data or, as in the current study, further stress the maternal-fetal system with triplets. Herein, we surgically separated the intercornual connections and isolated one uterine horn to more closely mimic the anatomy of human uterine anomalies (uterine bicornuate, unicornuate, or didelphys) in which the septum is devoid of vascular connections. The first report using this specific local ovine uterine horn isolation model was by Moor and Rowson [28]. They showed local early ovine embryo maintenance of corpus luteum function, which was subsequently shown to transpire via a local venoarterial pathway [27, 37]. However, the current study is the first to use this surgical model to evaluate uteroplacental function and fetal development in late ovine gestation.
Numerous studies have described a direct relationship between total placental size (i.e., placentome number and placentome weight) and morphology with nutrient transport ability and fetal growth [32, 38–42]. In the present study, control multifetal pregnancies increased overall placentome number from 66 ± 5 placentomes in singletons to 103 ± 4 in twins and 88 ± 8 in triplet pregnancies. These results are similar to other multifetal gestation studies with 20%–28% more placentomes reported [39, 43, 44]. This was likely due to placentomes formed in the uterine horns proximal to the uterotubal junction, with caruncles normally not activated in singleton pregnancies; these types of placentomes are thought to compensate for placental insufficiency [45]. In the current study, unilateral surgery decreased the total number of placentomes by 25% in singleton and 50% in twin pregnancies but not in triplets. This may be explained by the activation of these uterotubal placentomes in triplets but not singletons or twins. The overall decrease in number of placentomes in singleton and twin pregnancies was due to surgically reduced potential for placentation without the complete adaptation of uterotubal placentomes. This is consistent with the other unilateral models with 14%–46% fewer placentomes [33, 34, 36]. Regardless of the number of caruncles removed in any surgical model, the number of placentomes in the pregnancy is partly preserved, indicating placental adaptation.
After initial placental attachment, the number of placentomes do not change [30]. Therefore, increased placental weight (i.e., placental hypertrophy) and changes in morphology are two important ways in which the placenta can adapt to the increased fetal requirements seen throughout pregnancy. As fetal number increased, a dramatic increase in total placentomal weight was observed. Specifically, compared to singletons, the twins and triplets had a 68% and 120% increase in total placentomal weight, respectively. This was similar to a study by Vonnahme and coworkers [9] in which increasing fetal number from singletons to twins or triplets increased placental weight by 26%–52%, respectively, confirming the original observations of Barcroft [46], who compared singletons to twins. In contrast, others have reported little or no change in overall placentomal weight with increases in fetal number [43, 44]. Unilateral uterine surgery as a model for uterine anatomical anomalies did not alter total placentome weight in singleton or triplet but reduced total placentome weight in twin pregnancies. Data from other surgical uterine space restriction models are not in agreement with this study or each other. Caton et al. [36] and Ott et al. [34] found no change in total placentome weight, whereas Cefalo et al. [33] found a 23% increase in placentome weight. Differences among these studies are likely due to the lack of separation of singleton and twin data. Indeed, if we were to combine singletons and twins in the current study, we also would find no difference in total placentomal weight.
In the current study, pregnancies were classified into nonrestricted or uterine space-restricted based on the clear demarcation in the number of placentomes and placental weight per fetus (Fig. 3, A and B). Specifically, we defined the groups as nonrestricted and uterine space-restricted due to a 51% reduction in total placentome number and a 31% reduction in total placentome weight per fetus. These finding agree with those of Robinson et al. [31], who showed in the carunculectomy model that at least a 40% reduction in placentome number was necessary to produce IUGR. In the present model, we found that uterine space-restricted pregnancies had fewer A-type and more D-type placentomes and heavier C and D placentomes. This change in placental morphology and weight may be due to placental compensation (i.e., hypertrophy) described earlier. Current literature suggests that an increased percentage of C and D placentomes is an adaptation in an effort to increase nutrient delivery [32, 38, 42]. However, a functional difference between placentome types at the level of vascularity has recently been contested [47] and should be part of the focus in subsequent experiments with this particular model.
We also studied the effects of uterine space restriction on fetal morphometric parameters. IUGR in humans results in greater morbidity and mortality [48, 49]. Additionally, the Barker hypothesis proposes that IUGR is responsible for multiple adult-onset diseases due to permanent metabolic reprogramming [19, 48]. Two classifications of IUGR have been defined: symmetric and asymmetric. Symmetric, or proportionate, IUGR is associated with genetic abnormalities or first-trimester infections [20]. Asymmetric IUGR, however, is more common and is associated with placental insufficiency and asymmetric fetal linear growth, decreased fetal body and organ weight, and brain sparing [20]. In the present study, we observed complete fetal growth arrest between GD 120 and GD 130 such that asymmetric IUGR was clearly evident in uterine space-restricted fetuses by GD 130 with some signs manifesting as early as GD 120. This asymmetric growth is consistent with a disruption in the normal late gestation exponential growth [50].
As in humans, fetuses from uterine space-restricted pregnancies had lower body weight, crown-rump, abdominal girth, and thoracic girth. Restricted fetuses also had lighter hearts, kidneys, livers, spleens, and thymuses. Brain weights were similar between groups at GD 120 and GD 130 likely due to a redistribution of blood flow known as brain sparing, often seen in asymmetric IUGR [48, 51]. Lungs were an exception and were grown normally, consistent with humans, as fetal lung development is regulated by gestational age and cortisol levels but not by nutrient delivery [52–54]. Taken together, these findings are consistent with in utero predictors of human IUGR due to reduced fetal nutrient delivery and placental insufficiency [20].
In the current study, we first utilized the ratio of fetal weight to total placentome weight as an index of placental efficiency [55–60] and found increased placental efficiency in the uterine space-restricted ewes at 130 days. However, this traditional index of placental efficiency utilizes only placental weight and does not utilize true functional indices of placental insufficiency, such as placental clearance and/or delivery of nutrients and metabolites. In agreement with human IUGR, we observed at 130 days increased fetal creatinine [61], BUN [61], cholesterol [62], and ratios of maternal to fetal glucose and triglyceride levels [20, 63, 64], observations that were attributed to decreased placental ability to deliver nutrients and clear fetal metabolites. Some of these measures not only may have a causal relationship with IUGR but also could be the consequence of IUGR and/or dysfunctional fetal metabolism. However, best estimates of placental efficiency will require measurements of both uterine and placental blood flows and subsequent calculation of nutrient delivery rates and fetal nutrient consumption. Collectively, these observations demonstrate functional uteroplacental maladaptations in response to maternal uterine space restriction.
In conclusion, the present study provides a quantitative evaluation of two interacting factors, multifetal gestations and surgically created uterine anomaly, on placental adaptations and IUGR. While uterine anomalies might not always cause fetal IUGR, when it is combined with multifetal gestations, the severity of the detrimental effects of uterine space restriction are greatly compounded. The placenta appears to adapt to reductions in uterine space by increasing placentome numbers, by increasing individual placentomal weights, and through conversion from A to D anatomical types. Even as placental adaptations occur, fetal growth arrest is seen, giving rise to asymmetric IUGR. Thus, changes in placental efficiency with consequent reduction in fetal growth are fundamentally central to preserve a viable albeit compromised fetus. In short, this study provides a consistent model of IUGR that can be used to study numerous physiological and pathophysiologic processes involved in fetal and placental development.
Acknowledgments
Manuscript preparation assistance was provided by Sheikh Omar Jobe, Timothy Morschauser, Mayra Pastore, and Mary Sun. Graphical representation of the model was provided by Robert Gordon of the Department of Pediatrics. Technical support was provided by the Magness laboratory (Jason Austin, Kreg Grindle, Gladys Lopez, and Terry Phernetton), the Kling laboratory (Sharon Blohowiak and Jason Habeck), and Todd Taylor at the University of Wisconsin Arlington Farm Facility. This work is in partial fulfillment of the master's of science degree for the Endocrinology and Reproductive Physiology Training Program.
Footnotes
Supported by the University of Wisconsin Department of Pediatrics and NIH grants HL49210, HL87144, and HD38843 (R.R.M.).
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