Skip to main content
. Author manuscript; available in PMC: 2010 Oct 22.
Published in final edited form as: Immunity. 2009 Jan 29;30(2):204–217. doi: 10.1016/j.immuni.2008.11.014

Figure 6. IL-2-dependent signaling by T blasts bearing mutant IL-2Rβ.

Figure 6

(A) Spleen cells from the indicated mice were cultured with anti-CD3 for 48 hr. The T cells were isolated by magnetic bead sorting using anti-Thy-1.2, cultured in IL-4 for 24 hr, and then washed and “rested” in medium for 4 hr. These T cells were cultured with IL-2 for the indicated time and cytoplasmic extracts were prepared. Western blots were probed with mAbs to the indicated phospho-tyrosine proteins. mAb to unmodified STAT5 served as a loading control. Data are representative of 2 experiments. (B) The indicated anti-CD3 activated spleen cells were cultured in medium for 4 hr and then stimulated with IL-2 for the indicated time followed by staining for pSTAT5 and pS6. (C) Summary of all experiments where the MFI of pSTAT5 staining at 15 min by control IL-2Rβ+/+ or +/− was normalized to a value of 1 and used to compare staining by T cells bearing mutant IL-2Rβ. Data are the mean ± SEM of ≥3 mice/group <16 wks of age.