Table 1.
Viability, DNA concentration and transaminase levels of hepatocytes cultured in serum-free medium containing RPM
| RPM dose | 24 hr |
48 hr |
|||
|---|---|---|---|---|---|
| Viability percentage | DNA(μg/dish) | Viability percentage | DNA(μg/dish) | ALT (U/ml) | |
| Control | 95 ± 4a | 3.2 ± 0.2 | 96 ± 3 | 3.3 ± 0.2 | 10 ± 2 |
| RPM | |||||
| 0.4 ng/ml | 94 ± 3 | 3.3 ± 0.3 | 96 ± 4 | 3.3 ± 0.2 | 11 ± 4 |
| 4 ng/ml | 96 ± 4 | 3.2 ± 0.3 | 95 ± 5 | 3.2 ± 0.3 | 10 ± 3 |
| 10 ng/ml | 94 ± 3 | 3.3 ± 0.3 | 97 ± 3 | 3.3 ± 0.2 | 11 ± 2 |
a
Data expressed as the average from nine dishes and three different animals (mean ± S.D.).