Skip to main content
PMC home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1990 Mar;85(3):690–696. doi: 10.1172/JCI114493

In vitro responses to Leishmania antigens by lymphocytes from patients with leishmaniasis or Chagas' disease.

S G Reed 1, E M Carvalho 1, C H Sherbert 1, D P Sampaio 1, D M Russo 1, O Bacelar 1, D L Pihl 1, J M Scott 1, A Barral 1, K H Grabstein 1, et al.
PMCID: PMC296484  PMID: 2107208

Abstract

T cell responses are correlated with recovery from and resistance to leishmaniasis. Antigens of Leishmania chagasi were evaluated by determining their ability to elicit in vitro proliferation and cytokine production in peripheral blood lymphocytes and in T cell lines and clones from patients with histories of leishmaniasis or Chagas' disease. Antigens tested were selected by their reactivity with patient antibodies. Several of the antigens induced proliferative responses in peripheral blood lymphocytes from patients recovered from visceral or cutaneous leishmaniasis or with chronic Chagas' disease. Two purified glycoproteins, 30 and 42 kD, were consistently among the most effective in eliciting high proliferative responses and IL-2 production. Lymphocytes from a recovered visceral leishmaniasis patient were used to produce T cell lines against either the 30- or 42-kD antigen. Each of the lines responded to both of these antigens as well as to crude leishmania lysate. CD4+ T cell clones specific for either or both of these antigens were also isolated from a visceral leishmaniasis patient. In contrast, rabbit antisera produced against these two antigens were not crossreactive. Both antigens were effective in inducing the production of IFN-gamma from T cell lines from both leishmaniasis and Chagas' disease patients. These studies demonstrate the potential for defining parasite antigens with broad immunostimulatory capabilities.

Full text

PDF
690

Images in this article

691Image
on p.691
692Image
on p.692
694Image
on p.694
694Image
on p.694
695Image
on p.695
695Image
on p.695
695Image
on p.695
695Image
on p.695
695Image
on p.695

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Camargo M. E., Rebonato C. Cross-reactivity in fluorescence tests for Trypanosoma and Leishmania antibodies. A simple inhibition procedure to ensure specific results. Am J Trop Med Hyg. 1969 Jul;18(4):500–505. doi: 10.4269/ajtmh.1969.18.500. [DOI] [PubMed] [Google Scholar]
  2. Carvalho E. M., Badaró R., Reed S. G., Jones T. C., Johnson W. D., Jr Absence of gamma interferon and interleukin 2 production during active visceral leishmaniasis. J Clin Invest. 1985 Dec;76(6):2066–2069. doi: 10.1172/JCI112209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Carvalho E. M., Reed S. G., Johnson W. D., Jr Cross reactivity between Trypanosoma cruzi and Leishmania antigens in the lymphocyte blastogenesis assay. Trans R Soc Trop Med Hyg. 1987;81(1):82–84. doi: 10.1016/0035-9203(87)90291-4. [DOI] [PubMed] [Google Scholar]
  4. Carvalho E. M., Teixeira R. S., Johnson W. D., Jr Cell-mediated immunity in American visceral leishmaniasis: reversible immunosuppression during acute infection. Infect Immun. 1981 Aug;33(2):498–500. doi: 10.1128/iai.33.2.498-500.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Colomer-Gould V., Glvao Quintao L., Keithly J., Nogueira N. A common major surface antigen on amastigotes and promastigotes of Leishmania species. J Exp Med. 1985 Sep 1;162(3):902–916. doi: 10.1084/jem.162.3.902. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gillis S., Ferm M. M., Ou W., Smith K. A. T cell growth factor: parameters of production and a quantitative microassay for activity. J Immunol. 1978 Jun;120(6):2027–2032. [PubMed] [Google Scholar]
  7. Hurwitz J. L., Herber-Katz E., Hackett C. J., Gerhard W. Characterization of the murine TH response to influenza virus hemagglutinin: evidence for three major specificities. J Immunol. 1984 Dec;133(6):3371–3377. [PubMed] [Google Scholar]
  8. MANSON-BAHR P. E., HEISCH R. B., GARNHAM P. C. Studies in leishmanifasis in East Africa. IV. The Montenegro test in kala-azar in Kenya. Trans R Soc Trop Med Hyg. 1959 Sep;53:380–383. doi: 10.1016/0035-9203(59)90038-0. [DOI] [PubMed] [Google Scholar]
  9. MANSON-BAHR P. E. The leishmanin test and immunity in kala azar. East Afr Med J. 1961 Apr;38:165–167. [PubMed] [Google Scholar]
  10. Murray H. W., Rubin B. Y., Rothermel C. D. Killing of intracellular Leishmania donovani by lymphokine-stimulated human mononuclear phagocytes. Evidence that interferon-gamma is the activating lymphokine. J Clin Invest. 1983 Oct;72(4):1506–1510. doi: 10.1172/JCI111107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Reed S. G., Badaro R., Lloyd R. M. Identification of specific and cross-reactive antigens of Leishmania donovani chagasi by human infection sera. J Immunol. 1987 Mar 1;138(5):1596–1601. [PubMed] [Google Scholar]
  12. Reed S. G., Badaró R., Masur H., Carvalho E. M., Lorenco R., Lisboa A., Teixeira R., Johnson W. D., Jr, Jones T. C. Selection of a skin test antigen for American visceral leishmaniasis. Am J Trop Med Hyg. 1986 Jan;35(1):79–85. doi: 10.4269/ajtmh.1986.35.79. [DOI] [PubMed] [Google Scholar]
  13. Reed S. G. In vivo administration of recombinant IFN-gamma induces macrophage activation, and prevents acute disease, immune suppression, and death in experimental Trypanosoma cruzi infections. J Immunol. 1988 Jun 15;140(12):4342–4347. [PubMed] [Google Scholar]
  14. Russell D. G., Alexander J. Effective immunization against cutaneous leishmaniasis with defined membrane antigens reconstituted into liposomes. J Immunol. 1988 Feb 15;140(4):1274–1279. [PubMed] [Google Scholar]
  15. Santos-Buch C. A. American trypanosomiasis: Chagas' disease. Int Rev Exp Pathol. 1979;19:63–100. [PubMed] [Google Scholar]
  16. Tanowitz H. B., Amole B., Herman R., Wittner M. Protection against fatal murine Chagas' disease with a Leishmania braziliensis panamensis stock. Am J Trop Med Hyg. 1986 Sep;35(5):937–941. doi: 10.4269/ajtmh.1986.35.937. [DOI] [PubMed] [Google Scholar]
  17. Taswell C. Limiting dilution assays for the determination of immunocompetent cell frequencies. I. Data analysis. J Immunol. 1981 Apr;126(4):1614–1619. [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES