Fig. 1.

Multiple sequence and structural alignment for seven families of the C2 domain. The sequences are represented by an abbreviation of species name followed by Genbank GI and PDB ID. The consensus in 65% of the sequences shown below the alignment is derived using the following amino acid classes: a, aromatic (FHWY, black on orange); b, big (EFHIKLMQRWY, green on grey); h, hydrophobic (ACFGHILMTVWY, yellow on dark grey); l, aliphatic (ILV, red on grey); p, polar (CDEHKNQRST, blue on grey); s, small (ACDGNPSTV, cyan on dark grey); t, tiny (AGS, cyan on dark grey). Secondary structures for these C2 families, that were derived from PDB structures or predicted using the Jpred program, are indicated above the alignment of each own family (E in blue, β sheet; H in red, α helix). For each C2 family, conserved or specific residues that are potentially involved in binding membrane lipid are labeled on the top of the alignment and highlighted with the blue background for basic residues, the red background for acidic residues, and the orange background for aromatic residues. Non-conserved or subfamily-specific are indicated in italic. The numbers in bracket are indicative of the excluded residues from sequences. For complete multiple sequence alignments of AIDA-C2, B9-C2, DOCK-C2 and NT-C2 families refer to supplemental Figs. S1 to S4. Species name abbreviations: Ag, Anopheles gambiae; At, Arabidopsis thaliana; Ce, Caenorhabditis elegans; Cn, Cryptococcus neoformans; Cr, Chlamydomonas reinhardtii; Dd, Dictyostelium discoideum; Eh, Entamoeba histolytica; Hs, Homo sapiens; Lt, Lachancea thermotolerans; Mb, Monosiga brevicollis; Mi Micromonas sp.; Mt, Medicago truncatula; Nv, Nematostella vectensis; Ot, Ostreococcus tauri; Pi, Phytophthora infestans; Pm, Perkinsus marinus; Ps, Pichia stipitis; Pt, Paramecium tetraurelia; Sp, Strongylocentrotus purpuratus; Ta, Trichoplax adhaerens; Tb, Trypanosoma brucei; Tt, Tetrahymena thermophila; Tv, Trichomonas vaginalis.