Skip to main content
PMC home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1990 Apr;85(4):1006–1013. doi: 10.1172/JCI114530

Inhibition of bicarbonate absorption by peptide hormones and cyclic adenosine monophosphate in rat medullary thick ascending limb.

D W Good 1
PMCID: PMC296529  PMID: 2318960

Abstract

In vitro microperfusion experiments were performed to examine the effects of peptide hormones on bicarbonate and ammonium transport by the medullary thick ascending limb (MTAL) of the rat. Arginine vasopressin (AVP; 2.8 X 10(-10) M in the bath) reduced bicarbonate absorption by 50% (from 7.8 to 3.7 pmol/min per mm). AVP caused a similar reduction in bicarbonate absorption in tubules perfused with 10(-4) M furosemide to inhibit net NaCl absorption. Glucagon (2 X 10(-9) M in the bath) also reduced bicarbonate absorption (from 11.7 to 7.6 pmol/min per mm). The inhibition of bicarbonate absorption could be reproduced with either exogenous 8-bromo-cAMP or forskolin. With 8-bromo-cAMP (10(-3) M) in the bath, addition of vasopressin to the bath did not significantly affect bicarbonate absorption. PTH significantly inhibited bicarbonate absorption, but the extent of inhibition was less than that observed with either AVP or glucagon. Vasopressin had no effect on net ammonium absorption in MTAL perfused and bathed with 4 mM NH4Cl. These findings indicate that: (a) vasopressin, glucagon, and PTH directly inhibit bicarbonate absorption in the MTAL of the rat; (b) this inhibition occurs independent of effects on net NaCl absorption and appears to be mediated in part by cAMP; and (c) HCO3- and NH4+ absorption can be regulated independently in the MTAL.

Full text

PDF
1006

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beck N., Kim K. S., Wolak M., Davis B. B. Inhibition of carbonic anhydrase by parathyroid hormone and cyclic AMP in rat renal cortex in vitro. J Clin Invest. 1975 Jan;55(1):149–156. doi: 10.1172/JCI107905. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bengele H. H., McNamara E. R., Alexander E. A. Effect of acute thyroparathyroidectomy on nephron acidification. Am J Physiol. 1984 May;246(5 Pt 2):F569–F574. doi: 10.1152/ajprenal.1984.246.5.F569. [DOI] [PubMed] [Google Scholar]
  3. Bichara M., Mercier O., Houillier P., Paillard M., Leviel F. Effects of antidiuretic hormone on urinary acidification and on tubular handling of bicarbonate in the rat. J Clin Invest. 1987 Sep;80(3):621–630. doi: 10.1172/JCI113114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bichara M., Mercier O., Paillard M., Leviel F. Effects of parathyroid hormone on urinary acidification. Am J Physiol. 1986 Sep;251(3 Pt 2):F444–F453. doi: 10.1152/ajprenal.1986.251.3.F444. [DOI] [PubMed] [Google Scholar]
  5. Cole J. A., Eber S. L., Poelling R. E., Thorne P. K., Forte L. R. A dual mechanism for regulation of kidney phosphate transport by parathyroid hormone. Am J Physiol. 1987 Aug;253(2 Pt 1):E221–E227. doi: 10.1152/ajpendo.1987.253.2.E221. [DOI] [PubMed] [Google Scholar]
  6. Culpepper R. M., Andreoli T. E. PGE2, forskolin, and cholera toxin interactions in modulating NaCl transport in mouse mTALH. Am J Physiol. 1984 Nov;247(5 Pt 2):F784–F792. doi: 10.1152/ajprenal.1984.247.5.F784. [DOI] [PubMed] [Google Scholar]
  7. Delahousse M., Mercier O., Bichara M., Paillard M., Leviel F., Assan R. Glucagon inhibits urinary acidification in the rat. Am J Physiol. 1988 May;254(5 Pt 2):F762–F769. doi: 10.1152/ajprenal.1988.254.5.F762. [DOI] [PubMed] [Google Scholar]
  8. Garvin J. L., Burg M. B., Knepper M. A. Active NH4+ absorption by the thick ascending limb. Am J Physiol. 1988 Jul;255(1 Pt 2):F57–F65. doi: 10.1152/ajprenal.1988.255.1.F57. [DOI] [PubMed] [Google Scholar]
  9. Good D. W. Active absorption of NH4+ by rat medullary thick ascending limb: inhibition by potassium. Am J Physiol. 1988 Jul;255(1 Pt 2):F78–F87. doi: 10.1152/ajprenal.1988.255.1.F78. [DOI] [PubMed] [Google Scholar]
  10. Good D. W. Effects of potassium on ammonia transport by medullary thick ascending limb of the rat. J Clin Invest. 1987 Nov;80(5):1358–1365. doi: 10.1172/JCI113213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Good D. W., Knepper M. A. Ammonia transport in the mammalian kidney. Am J Physiol. 1985 Apr;248(4 Pt 2):F459–F471. doi: 10.1152/ajprenal.1985.248.4.F459. [DOI] [PubMed] [Google Scholar]
  12. Good D. W., Knepper M. A., Burg M. B. Ammonia and bicarbonate transport by thick ascending limb of rat kidney. Am J Physiol. 1984 Jul;247(1 Pt 2):F35–F44. doi: 10.1152/ajprenal.1984.247.1.F35. [DOI] [PubMed] [Google Scholar]
  13. Good D. W. Sodium-dependent bicarbonate absorption by cortical thick ascending limb of rat kidney. Am J Physiol. 1985 Jun;248(6 Pt 2):F821–F829. doi: 10.1152/ajprenal.1985.248.6.F821. [DOI] [PubMed] [Google Scholar]
  14. Greger R. Ion transport mechanisms in thick ascending limb of Henle's loop of mammalian nephron. Physiol Rev. 1985 Jul;65(3):760–797. doi: 10.1152/physrev.1985.65.3.760. [DOI] [PubMed] [Google Scholar]
  15. Hebert S. C., Reeves W. B., Molony D. A., Andreoli T. E. The medullary thick limb: function and modulation of the single-effect multiplier. Kidney Int. 1987 Feb;31(2):580–589. doi: 10.1038/ki.1987.38. [DOI] [PubMed] [Google Scholar]
  16. Hulter H. N. Effects and interrelationships of PTH, Ca2+, vitamin D, and Pi in acid-base homeostasis. Am J Physiol. 1985 Jun;248(6 Pt 2):F739–F752. doi: 10.1152/ajprenal.1985.248.6.F739. [DOI] [PubMed] [Google Scholar]
  17. Iino Y., Burg M. B. Effect of parathyroid hormone on bicarbonate absorption by proximal tubules in vitro. Am J Physiol. 1979 Apr;236(4):F387–F391. doi: 10.1152/ajprenal.1979.236.4.F387. [DOI] [PubMed] [Google Scholar]
  18. Imbert-Teboul M., Chabardès D., Clique A., Montégut M., Morel F. Ontogenesis of hormone-dependent adenylate cyclase in isolated rat nephron segments. Am J Physiol. 1984 Aug;247(2 Pt 2):F316–F325. doi: 10.1152/ajprenal.1984.247.2.F316. [DOI] [PubMed] [Google Scholar]
  19. Imbert-Teboul M., Chabardès D., Montégut M., Clique A., Morel F. Vasopressin-dependent adenylate cyclase activities in the rat kidney medulla: evidence for two separate sites of action. Endocrinology. 1978 Apr;102(4):1254–1261. doi: 10.1210/endo-102-4-1254. [DOI] [PubMed] [Google Scholar]
  20. Kinne R., Kinne-Saffran E., Schütz H., Schölermann B. Ammonium transport in medullary thick ascending limb of rabbit kidney: involvement of the Na+,K+,Cl(-)-cotransporter. J Membr Biol. 1986;94(3):279–284. doi: 10.1007/BF01869723. [DOI] [PubMed] [Google Scholar]
  21. Knepper M. A., Packer R., Good D. W. Ammonium transport in the kidney. Physiol Rev. 1989 Jan;69(1):179–249. doi: 10.1152/physrev.1989.69.1.179. [DOI] [PubMed] [Google Scholar]
  22. Knepper M. A. Urea transport in isolated thick ascending limbs and collecting ducts from rats. Am J Physiol. 1983 Nov;245(5 Pt 1):F634–F639. doi: 10.1152/ajprenal.1983.245.5.F634. [DOI] [PubMed] [Google Scholar]
  23. Lowance D. C., Garfinkel H. B., Mattern W. D., Schwartz W. B. The effect of chronic hypotonic volume expansion on the renal regulation of acid-base equilibrium. J Clin Invest. 1972 Nov;51(11):2928–2940. doi: 10.1172/JCI107117. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. McKinney T. D., Myers P. Bicarbonate transport by proximal tubules: effect of parathyroid hormone and dibutyryl cyclic AMP. Am J Physiol. 1980 Mar;238(3):F166–F174. doi: 10.1152/ajprenal.1980.238.3.F166. [DOI] [PubMed] [Google Scholar]
  25. Molony D. A., Reeves W. B., Hebert S. C., Andreoli T. E. ADH increases apical Na+, K+, 2Cl- entry in mouse medullary thick ascending limbs of Henle. Am J Physiol. 1987 Jan;252(1 Pt 2):F177–F187. doi: 10.1152/ajprenal.1987.252.1.F177. [DOI] [PubMed] [Google Scholar]
  26. Morel F., Doucet A. Hormonal control of kidney functions at the cell level. Physiol Rev. 1986 Apr;66(2):377–468. doi: 10.1152/physrev.1986.66.2.377. [DOI] [PubMed] [Google Scholar]
  27. Morel F., Imbert-Teboul M., Chabardès D. Receptors to vasopressin and other hormones in the mammalian kidney. Kidney Int. 1987 Feb;31(2):512–520. doi: 10.1038/ki.1987.30. [DOI] [PubMed] [Google Scholar]
  28. Pollock A. S., Warnock D. G., Strewler G. J. Parathyroid hormone inhibition of Na+-H+ antiporter activity in a cultured renal cell line. Am J Physiol. 1986 Feb;250(2 Pt 2):F217–F225. doi: 10.1152/ajprenal.1986.250.2.F217. [DOI] [PubMed] [Google Scholar]
  29. Reuss L., Petersen K. U. Cyclic AMP inhibits Na+/H+ exchange at the apical membrane of Necturus gallbladder epithelium. J Gen Physiol. 1985 Mar;85(3):409–429. doi: 10.1085/jgp.85.3.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Sasaki S., Imai M. Effects of vasopressin on water and NaCl transport across the in vitro perfused medullary thick ascending limb of Henle's loop of mouse, rat, and rabbit kidneys. Pflugers Arch. 1980 Feb;383(3):215–221. doi: 10.1007/BF00587521. [DOI] [PubMed] [Google Scholar]
  31. Tomita K., Pisano J. J., Burg M. B., Knepper M. A. Effects of vasopressin and bradykinin on anion transport by the rat cortical collecting duct. Evidence for an electroneutral sodium chloride transport pathway. J Clin Invest. 1986 Jan;77(1):136–141. doi: 10.1172/JCI112268. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Torikai S., Wang M. S., Klein K. L., Kurokawa K. Adenylate cyclase and cell cyclic AMP of rat cortical thick ascending limb of Henle. Kidney Int. 1981 Nov;20(5):649–654. doi: 10.1038/ki.1981.189. [DOI] [PubMed] [Google Scholar]
  33. Trinh-Trang-Tan M. M., Bouby N., Coutaud C., Bankir L. Quick isolation of rat medullary thick ascending limbs. Enzymatic and metabolic characterization. Pflugers Arch. 1986 Aug;407(2):228–234. doi: 10.1007/BF00580681. [DOI] [PubMed] [Google Scholar]
  34. Troyer D. A., Schwertz D. W., Kreisberg J. I., Venkatachalam M. A. Inositol phospholipid metabolism in the kidney. Annu Rev Physiol. 1986;48:51–71. doi: 10.1146/annurev.ph.48.030186.000411. [DOI] [PubMed] [Google Scholar]
  35. Wall S. M., Sands J. M., Flessner M. F., Nonoguchi H., Spring K. R., Knepper M. A. Net acid transport by isolated perfused inner medullary collecting ducts. Am J Physiol. 1990 Jan;258(1 Pt 2):F75–F84. doi: 10.1152/ajprenal.1990.258.1.F75. [DOI] [PubMed] [Google Scholar]
  36. Weinman E. J., Shenolikar S., Kahn A. M. cAMP-associated inhibition of Na+-H+ exchanger in rabbit kidney brush-border membranes. Am J Physiol. 1987 Jan;252(1 Pt 2):F19–F25. doi: 10.1152/ajprenal.1987.252.1.F19. [DOI] [PubMed] [Google Scholar]
  37. Work J., Galla J. H., Booker B. B., Schafer J. A., Luke R. G. Effect of ADH on chloride reabsorption in the loop of Henle of the Brattleboro rat. Am J Physiol. 1985 Nov;249(5 Pt 2):F698–F703. doi: 10.1152/ajprenal.1985.249.5.F698. [DOI] [PubMed] [Google Scholar]
  38. de Rouffignac C., Elalouf J. M., Roinel N. Physiological control of the urinary concentrating mechanism by peptide hormones. Kidney Int. 1987 Feb;31(2):611–620. doi: 10.1038/ki.1987.42. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES