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. Author manuscript; available in PMC: 2010 Oct 28.
Published in final edited form as: Gastroenterology. 1986 Jul;91(1):182–188. doi: 10.1016/0016-5085(86)90456-7

Figure 1.

Figure 1

Specific binding of [3H]E2 in hepatic cytosol of the male rat. Aliquots (200 μl)of cytosol (5 mg protein/ml) precipitated with protamine sulfate were incubated with six different concentrations of [3H]E2 (0.15–3.0 nM) for 18 h at 0°C in the absence (total binding) and presence (nonspecific binding) of 100-fold excess of unlabeled E2. Specific binding was calculated by subtracting nonspecific binding from total binding. Each point is the mean ± SEM of triplicate determinations. The insert shows the Scatchard analysis of [3H]E2 specific binding.