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The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1990 May;85(5):1379–1391. doi: 10.1172/JCI114582

Molecular cloning, expression, and chromosome 19 localization of a human Ro/SS-A autoantigen.

D P McCauliffe 1, F A Lux 1, T S Lieu 1, I Sanz 1, J Hanke 1, M M Newkirk 1, L L Bachinski 1, Y Itoh 1, M J Siciliano 1, M Reichlin 1, et al.
PMCID: PMC296583  PMID: 2332496

Abstract

Ro/SS-A antibodies are found in a number of human autoimmune disorders including Sjogren's syndrome and several systemic lupus erythematosus-related disorders. These heterogeneous autoantibodies are known to recognize several distinct cellular antigens. With synthetic oligonucleotides corresponding to amino acid sequence information we have isolated a full-length cDNA clone which encodes a human Ro ribonucleoprotein autoantigen. The 1,890-base pair clone contains an open reading frame that encodes a 417-amino acid, 48-kD polypeptide that migrates aberrantly at 60 kD by SDS-PAGE. Rabbit antibodies raised against this protein's recently described amino-terminal epitope react with a previously identified 52-kD human Ro protein and immunoprecipitate the human cytoplasmic RNAs. Ultraviolet light cross-linking studies suggest that this Ro protein binds each of the four major human cytoplasmic RNAs. The deduced amino acid sequence is 63% homologous to an Onchocerca volvulus antigen. Southern filter hybridization analysis indicates that this gene is not highly polymorphic and exists as a single copy in the human genome. Chromosomal localization studies place this gene on the short arm of chromosome 19 near the gene encoding the low density lipoprotein receptor.

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Selected References

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