Skip to main content
NCBI home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1990 May;85(5):1421–1426. doi: 10.1172/JCI114586

Constitutive and inducible expression of HLA class II determinants by human osteoblast-like cells in vitro.

H Skjødt 1, D E Hughes 1, P R Dobson 1, R G Russell 1
PMCID: PMC296587  PMID: 2110190

Abstract

Activated immune cells release cytokines which modulate the activity of bone cells in vitro. Expression of major histocompatibility complex (HLA in humans) class II determinants on bone surface cells may be important in local immune cell activation. In this study, expression of HLA-DR and DQ by cultured human bone cells (HBC) derived from normal trabecular bone surfaces was assessed by fluorescence-activated cell sorter (FACS) analysis and immunoperoxidase techniques using monoclonal antibodies. A subset of HBC (10-30%) expressed DR constitutively while 5-15% displayed DQ during long-term culture. HBC lacked a number of monocyte and lymphocyte markers. In addition, both DR+ and DR- HBC (FACS separated) produced osteocalcin stimulated by 1,25-dihydroxyvitamin D2 (1,25(OH)2D3). This suggests that both phenotypes belong to the osteoblast lineage. The number of DR+ HBC was increased by interferon-gamma (IFN gamma; 40-95% DR+ cells) whereas DQ+ HBC remained unchanged or was slightly increased (5-20% DQ+ cells). Moreover, 1,25(OH)2D3 enhanced IFN gamma-induced DR expression and at high concentration (10(-7) M) augmented DR expression by itself. Other major osteotropic factors, parathyroid hormone, interleukin 1, and calcitonin, did not affect HBC DR expression. The findings suggest that HBC may participate in activation of the immune system and that some osteotropic factors may regulate this function.

Full text

PDF
1421

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amento E. P., Bhan A. K., McCullagh K. G., Krane S. M. Influences of gamma interferon on synovial fibroblast-like cells. Ia induction and inhibition of collagen synthesis. J Clin Invest. 1985 Aug;76(2):837–848. doi: 10.1172/JCI112041. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ashton B. A., Abdullah F., Cave J., Williamson M., Sykes B. C., Couch M., Poser J. W. Characterization of cells with high alkaline phosphatase activity derived from human bone and marrow: preliminary assessment of their osteogenicity. Bone. 1985;6(5):313–319. doi: 10.1016/8756-3282(85)90321-7. [DOI] [PubMed] [Google Scholar]
  3. Beresford J. N., Gallagher J. A., Poser J. W., Russell R. G. Production of osteocalcin by human bone cells in vitro. Effects of 1,25(OH)2D3, 24,25(OH)2D3, parathyroid hormone, and glucocorticoids. Metab Bone Dis Relat Res. 1984;5(5):229–234. doi: 10.1016/0221-8747(84)90064-x. [DOI] [PubMed] [Google Scholar]
  4. Bertolini D. R., Nedwin G. E., Bringman T. S., Smith D. D., Mundy G. R. Stimulation of bone resorption and inhibition of bone formation in vitro by human tumour necrosis factors. Nature. 1986 Feb 6;319(6053):516–518. doi: 10.1038/319516a0. [DOI] [PubMed] [Google Scholar]
  5. Bozdech M. J., Bainton D. F. Identification of alpha-naphthyl butyrate esterase as a plasma membrane ectoenzyme of monocytes and as a discrete intracellular membrane-bounded organelle in lymphocytes. J Exp Med. 1981 Jan 1;153(1):182–195. doi: 10.1084/jem.153.1.182. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Burmester G. R., Jahn B., Rohwer P., Zacher J., Winchester R. J., Kalden J. R. Differential expression of Ia antigens by rheumatoid synovial lining cells. J Clin Invest. 1987 Sep;80(3):595–604. doi: 10.1172/JCI113111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Carrington M. N., Tharp-Hiltbold B., Knoth J., Ward F. E. 1,25-Dihydroxyvitamin D3 decreases expression of HLA class II molecules in a melanoma cell line. J Immunol. 1988 Jun 1;140(11):4013–4018. [PubMed] [Google Scholar]
  8. Dewhirst F. E., Stashenko P. P., Mole J. E., Tsurumachi T. Purification and partial sequence of human osteoclast-activating factor: identity with interleukin 1 beta. J Immunol. 1985 Oct;135(4):2562–2568. [PubMed] [Google Scholar]
  9. Dinarello C. A., Mier J. W. Lymphokines. N Engl J Med. 1987 Oct 8;317(15):940–945. doi: 10.1056/NEJM198710083171506. [DOI] [PubMed] [Google Scholar]
  10. Farley J. R., Tarbaux N. M., Hall S. L., Linkhart T. A., Baylink D. J. The anti-bone-resorptive agent calcitonin also acts in vitro to directly increase bone formation and bone cell proliferation. Endocrinology. 1988 Jul;123(1):159–167. doi: 10.1210/endo-123-1-159. [DOI] [PubMed] [Google Scholar]
  11. Friedman J., Raisz L. G. Thyrocalcitonin: inhibitor of bone resorption in tissue culture. Science. 1965 Dec 10;150(3702):1465–1467. doi: 10.1126/science.150.3702.1465. [DOI] [PubMed] [Google Scholar]
  12. Geppert T. D., Lipsky P. E. Antigen presentation by interferon-gamma-treated endothelial cells and fibroblasts: differential ability to function as antigen-presenting cells despite comparable Ia expression. J Immunol. 1985 Dec;135(6):3750–3762. [PubMed] [Google Scholar]
  13. Gonwa T. A., Stobo J. D. Differential expression of Ia molecules by human monocytes. J Clin Invest. 1984 Sep;74(3):859–866. doi: 10.1172/JCI111503. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Gowen M., Nedwin G. E., Mundy G. R. Preferential inhibition of cytokine-stimulated bone resorption by recombinant interferon gamma. J Bone Miner Res. 1986 Oct;1(5):469–474. doi: 10.1002/jbmr.5650010511. [DOI] [PubMed] [Google Scholar]
  15. Jacobs D. M., Morrison D. C. Inhibition of the mitogenic response to lipopolysaccharide (LPS) in mouse spleen cells by polymyxin B. J Immunol. 1977 Jan;118(1):21–27. [PubMed] [Google Scholar]
  16. Jahn B., Burmester G. R., Schmid H., Weseloh G., Rohwer P., Kalden J. R. Changes in cell surface antigen expression on human articular chondrocytes induced by gamma-interferon. Induction of Ia antigens. Arthritis Rheum. 1987 Jan;30(1):64–74. doi: 10.1002/art.1780300109. [DOI] [PubMed] [Google Scholar]
  17. Knight S. C., Fryer P., Griffiths S., Harding B. Class II histocompatibility antigens on human dendritic cells. Immunology. 1987 May;61(1):21–27. [PMC free article] [PubMed] [Google Scholar]
  18. Morel P. A., Manolagas S. C., Provvedini D. M., Wegmann D. R., Chiller J. M. Interferon-gamma-induced IA expression in WEHI-3 cells is enhanced by the presence of 1,25-dihydroxyvitamin D3. J Immunol. 1986 Mar 15;136(6):2181–2186. [PubMed] [Google Scholar]
  19. Royer H. D., Reinherz E. L. T lymphocytes: ontogeny, function, and relevance to clinical disorders. N Engl J Med. 1987 Oct 29;317(18):1136–1142. doi: 10.1056/NEJM198710293171807. [DOI] [PubMed] [Google Scholar]
  20. Skjødt H., Møller T., Freiesleben S. F. Human osteoblast-like cells expressing MHC class II determinants stimulate allogeneic and autologous peripheral blood mononuclear cells and function as antigen-presenting cells. Immunology. 1989 Nov;68(3):416–420. [PMC free article] [PubMed] [Google Scholar]
  21. Thomson B. M., Mundy G. R., Chambers T. J. Tumor necrosis factors alpha and beta induce osteoblastic cells to stimulate osteoclastic bone resorption. J Immunol. 1987 Feb 1;138(3):775–779. [PubMed] [Google Scholar]
  22. Thomson B. M., Saklatvala J., Chambers T. J. Osteoblasts mediate interleukin 1 stimulation of bone resorption by rat osteoclasts. J Exp Med. 1986 Jul 1;164(1):104–112. doi: 10.1084/jem.164.1.104. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Tiku M. L., Liu S., Weaver C. W., Teodorescu M., Skosey J. L. Class II histocompatibility antigen-mediated immunologic function of normal articular chondrocytes. J Immunol. 1985 Nov;135(5):2923–2928. [PubMed] [Google Scholar]
  24. Vaes G. Cellular biology and biochemical mechanism of bone resorption. A review of recent developments on the formation, activation, and mode of action of osteoclasts. Clin Orthop Relat Res. 1988 Jun;(231):239–271. [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES