Skip to main content
NCBI home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1990 Nov;86(5):1474–1479. doi: 10.1172/JCI114864

Mechanism of acid-induced release of secretin in rats. Presence of a secretin-releasing peptide.

P Li 1, K Y Lee 1, T M Chang 1, W Y Chey 1
PMCID: PMC296892  PMID: 2243126

Abstract

In fasting rats, intraduodenal infusion of dilute hydrochloric acid results in significant increases in both pancreatic exocrine secretion and plasma concentration of secretin. To test the hypothesis that acid-induced release of secretin is mediated by a secretin-releasing factor (S-RF), anesthetized rats were prepared with pyloric ligation, duodenal and jejunal cannulas, and pancreatic duct cannulas. Donor rats were infused intraduodenally with 0.01 N HCl, 0.15 M NaCl, or a combination of 0.01 N HCl and 0.05 N NaHCO3 at 0.3 ml/min for 1.5 h, and the perfusates were collected via jejunal cannulas. The perfusates with pH adjusted to 6.0 were concentrated threefold and infused into the duodena of recipient rats. The concentrate of acid perfusate (CAP) significantly increased both pancreatic volume flow and bicarbonate output and plasma concentration of secretin, whereas concentrates of the saline perfusate (CSP) or the perfusate of a combination of 0.01 N HCl and 0.05 N NaHCO3 (CABP) did not influence pancreatic secretion or plasma concentration of secretin. The increased pancreatic secretion by CAP was attributed to increased circulating secretin because when secretin was immunoneutralized by a rabbit antisecretin serum, CAP-stimulated pancreatic secretion was abolished. The bioactivity of CAP was trypsin-sensitive and heat stable. The active substance in CAP had a molecular weight of less than 5,000 and greater than 1,000, as determined by ultrafiltration and bioassay. In conclusion, dilute HCl releases an S-RF into the upper small intestinal lumen to stimulate release of secretin. This substance, with molecular weight of less than 5,000, is heat stable and trypsin sensitive. Thus, the acid-stimulated release of secretin is mediated by a secretin-releasing peptide in the upper small intestinal lumen.

Full text

PDF
1474

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANNIS D., HALLENBECK G. A. Effect of excluding pancreatic juice from duodenum on secretory response of pancreas to a meal. Proc Soc Exp Biol Med. 1951 Jul;77(3):383–385. doi: 10.3181/00379727-77-18785. [DOI] [PubMed] [Google Scholar]
  2. Bayliss W. M., Starling E. H. The mechanism of pancreatic secretion. J Physiol. 1902 Sep 12;28(5):325–353. doi: 10.1113/jphysiol.1902.sp000920. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chang T. M., Chey W. Y., Kim M. S., Lee K. Y. The release of biologically active secretin-like immunoreactivity into duodenal lumen of dogs. J Physiol. 1981 Nov;320:393–401. doi: 10.1113/jphysiol.1981.sp013957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chang T. M., Chey W. Y. Radioimmunoassay of cholecystokinin. Dig Dis Sci. 1983 May;28(5):456–468. doi: 10.1007/BF02430535. [DOI] [PubMed] [Google Scholar]
  5. Chang T. M., Chey W. Y. Radioimmunoassay of secretin. A critical review and current status. Dig Dis Sci. 1980 Jul;25(7):529–552. doi: 10.1007/BF01315215. [DOI] [PubMed] [Google Scholar]
  6. Chey W. Y., Kim M. S., Lee K. Y., Chang T. M. Effect of rabbit antisecretin serum on postprandial pancreatic secretion in dogs. Gastroenterology. 1979 Dec;77(6):1268–1275. [PubMed] [Google Scholar]
  7. Chey W. Y., Lee Y. H., Hendricks J. G., Rhodes R. A., Tai H. H. Plasma secretin concentrations in fasting and postprandial state in man. Am J Dig Dis. 1978 Nov;23(11):981–988. doi: 10.1007/BF01263096. [DOI] [PubMed] [Google Scholar]
  8. Fölsch U. R., Cantor P., Wilms H. M., Schafmayer A., Becker H. D., Creutzfeldt W. Role of cholecystokinin in the negative feedback control of pancreatic enzyme secretion in conscious rats. Gastroenterology. 1987 Feb;92(2):449–458. doi: 10.1016/0016-5085(87)90141-7. [DOI] [PubMed] [Google Scholar]
  9. Green G. M., Lyman R. L. Feedback regulation of pancreatic enzyme secretion as a mechanism for trypsin inhibitor-induced hypersecretion in rats. Proc Soc Exp Biol Med. 1972 May;140(1):6–12. doi: 10.3181/00379727-140-36384. [DOI] [PubMed] [Google Scholar]
  10. HUMMEL B. C. A modified spectrophotometric determination of chymotrypsin, trypsin, and thrombin. Can J Biochem Physiol. 1959 Dec;37:1393–1399. [PubMed] [Google Scholar]
  11. Jordan P. H., Jr, Yip B. S. The presence of gastrin in fasting and stimulated gastric juice of man. Surgery. 1972 Sep;72(3):352–356. [PubMed] [Google Scholar]
  12. Kim M. S., Lee K. Y., Chey W. Y. Plasma secretin concentrations in fasting and postprandial states in dog. Am J Physiol. 1979 May;236(5):E539–E544. doi: 10.1152/ajpendo.1979.236.5.E539. [DOI] [PubMed] [Google Scholar]
  13. Lee K. Y., Zhou L., Ren X. S., Chang T. M., Chey W. Y. An important role of endogenous insulin on exocrine pancreatic secretion in rats. Am J Physiol. 1990 Feb;258(2 Pt 1):G268–G274. doi: 10.1152/ajpgi.1990.258.2.G268. [DOI] [PubMed] [Google Scholar]
  14. Li P., Lee K. Y., Ren X. S., Chang T. M., Chey W. Y. Effect of pancreatic proteases on plasma cholecystokinin, secretin, and pancreatic exocrine secretion in response to sodium oleate. Gastroenterology. 1990 Jun;98(6):1642–1648. doi: 10.1016/0016-5085(90)91102-c. [DOI] [PubMed] [Google Scholar]
  15. Louie D. S., May D., Miller P., Owyang C. Cholecystokinin mediates feedback regulation of pancreatic enzyme secretion in rats. Am J Physiol. 1986 Feb;250(2 Pt 1):G252–G259. doi: 10.1152/ajpgi.1986.250.2.G252. [DOI] [PubMed] [Google Scholar]
  16. Lu L., Louie D., Owyang C. A cholecystokinin releasing peptide mediates feedback regulation of pancreatic secretion. Am J Physiol. 1989 Feb;256(2 Pt 1):G430–G435. doi: 10.1152/ajpgi.1989.256.2.G430. [DOI] [PubMed] [Google Scholar]
  17. Schaffalitzky de Muckadell O. B., Fahrenkrug J. Secretion pattern of secretin in man: regulation by gastric acid. Gut. 1978 Sep;19(9):812–818. doi: 10.1136/gut.19.9.812. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Schwartz M. Z., Storozuk R. B. Enhancement of small intestine absorption by intraluminal gastrin. J Surg Res. 1986 May;40(5):421–425. doi: 10.1016/0022-4804(86)90209-x. [DOI] [PubMed] [Google Scholar]
  19. Schwartz M. Z., Storozuk R. B. The influence of gastrin on gastrointestinal function. J Pediatr Surg. 1986 Dec;21(12):1123–1127. doi: 10.1016/0022-3468(86)90023-0. [DOI] [PubMed] [Google Scholar]
  20. Shiratori K., Chen Y. F., Chey W. Y., Lee K. Y., Chang T. M. Mechanism of increased exocrine pancreatic secretion in pancreatic juice-diverted rats. Gastroenterology. 1986 Nov;91(5):1171–1178. doi: 10.1016/s0016-5085(86)80013-0. [DOI] [PubMed] [Google Scholar]
  21. Shiratori K., Jo Y. H., Lee K. Y., Chang T. M., Chey W. Y. Effect of pancreatic juice and trypsin on oleic acid-stimulated pancreatic secretion and plasma secretin in dogs. Gastroenterology. 1989 May;96(5 Pt 1):1330–1336. doi: 10.1016/s0016-5085(89)80020-4. [DOI] [PubMed] [Google Scholar]
  22. Sninsky C. A., Wolfe M. M., McGuigan J. E., Mathias J. R. Alterations in motor function of the small intestine from intravenous and intraluminal cholecystokinin. Am J Physiol. 1984 Dec;247(6 Pt 1):G724–G728. doi: 10.1152/ajpgi.1984.247.6.G724. [DOI] [PubMed] [Google Scholar]
  23. Sun G., Lee K. Y., Chang T. M., Chey W. Y. Effect of pancreatic juice diversion on secretin release in rats. Gastroenterology. 1989 Apr;96(4):1173–1179. doi: 10.1016/0016-5085(89)91638-7. [DOI] [PubMed] [Google Scholar]
  24. Uvnas-Wallensten K., Efendić S., Luft R. Release of somatostatin into the antral lumen of cats. Metabolism. 1978 Sep;27(9 Suppl 1):1233–1234. doi: 10.1016/0026-0495(78)90049-5. [DOI] [PubMed] [Google Scholar]
  25. Uvnäs-Wallensten K., Lundberg J. M., Efendic S. Dopaminergic control of antral gastrin and somatostatin release. Acta Physiol Scand. 1978 Jul;103(3):343–345. doi: 10.1111/j.1748-1716.1978.tb06224.x. [DOI] [PubMed] [Google Scholar]
  26. Yeo C. J., Jaffe B. M., Zinner M. J. Local regulation of blood flow in the feline jejunum. A possible role for endoluminally released substance P. J Clin Invest. 1982 Dec;70(6):1329–1333. doi: 10.1172/JCI110735. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES