Abstract
The γG-immunoglobulin (IgG) deposited on glomeruli of 32 percutaneous renal biopsies from 30 patients with various forms of renal disease was examined by fluorescent techniques to determine its light chain composition. Serum kappa: lambda light chain ratios were determined on 17 serum samples from 16 of the 30 patients.
Glomerular IgG staining with only antikappa antiserum, only antilambda antiserum, and with both antisera was demonstrated. No correlation of serum kappa: lambda light chain ratios with the type or types of light chains demonstrable on the glomeruli was observed. We concluded that the IgG deposited on glomeruli of patients with various forms of renal disease is not a nonspecific sample of the circulating immunoglobulin pool but may well reflect more specific selection, as in the deposition of specific antibody formed against exogenous or endogenous antigens during the development of the renal lesion.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BERNIER G. M., PUTNAM F. W. POLYMERISM, POLYMORPHISM, AND IMPURITIES IN BENCE-JONES PROTEINS. Biochim Biophys Acta. 1964 May 11;86:295–308. doi: 10.1016/0304-4165(64)90056-x. [DOI] [PubMed] [Google Scholar]
- Bernier G. M., Cebra J. J. Frequency distribution of alpha, gamma, kappa and lambda polypeptide chains in human lymphoid tissues. J Immunol. 1965 Aug;95(2):246–253. [PubMed] [Google Scholar]
- DIXON F. J., VAZQUEZ J. J., WEIGLE W. O., COCHRANE C. G. Pathogenesis of serum sickness. AMA Arch Pathol. 1958 Jan;65(1):18–28. [PubMed] [Google Scholar]
- FREEDMAN P., MARKOWITZ A. S. Isolation of antibody-like gamma-globulin from lupus glomeruli. Br Med J. 1962 Apr 28;1(5286):1175–1178. doi: 10.1136/bmj.1.5286.1175. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HEYMANN W., HACKEL D. B., HARWOOD S., WILSON S. G., HUNTER J. L. Production of nephrotic syndrome in rats by Freund's adjuvants and rat kidney suspensions. Proc Soc Exp Biol Med. 1959 Apr;100(4):660–664. doi: 10.3181/00379727-100-24736. [DOI] [PubMed] [Google Scholar]
- LEDDY J. P., BAKEMEIER R. F. STRUCTURAL ASPECTS OF HUMAN ERYTHROCYTE AUTOANTIBODIES. I. L CHAIN TYPES AND ELECTROPHORETIC DISPERSION. J Exp Med. 1965 Jan 1;121:1–17. doi: 10.1084/jem.121.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MANNIK M., KUNKEL H. G. LOCALIZATION OF ANTIBODIES IN GROUP I AND GROUP II GAMMA-GLOBULINS. J Exp Med. 1963 Nov 1;118:817–826. doi: 10.1084/jem.118.5.817. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MELLORS R. C. Histochemical demonstration of the in vivo localization of antibodies: antigenic components of the kidney and the pathogenesis of glomerulonephritis. J Histochem Cytochem. 1955 Jul;3(4):284–289. doi: 10.1177/3.4.284. [DOI] [PubMed] [Google Scholar]
- MICHAEL A. F., DRUMMOND K. N., VERNIER R. L., GOOD R. A. IMMUNOLOGIC BASIS OF RENAL DISEASE. Pediatr Clin North Am. 1964 Aug;11:685–721. doi: 10.1016/s0031-3955(16)31590-5. [DOI] [PubMed] [Google Scholar]
- McKelvey E. M., Fahey J. L. Immunoglobulin changes in disease: quantitation on the basis of heavy polypeptide chains, IgG (gammaG), IgA (gammaA), and IgM (gammaM), and of light polypeptide chains, type K (I) and type L (II). J Clin Invest. 1965 Nov;44(11):1778–1787. doi: 10.1172/JCI105285. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Michael A. F., Jr, Drummond K. N., Good R. A., Vernier R. L. Acute poststreptococcal glomerulonephritis: immune deposit disease. J Clin Invest. 1966 Feb;45(2):237–248. doi: 10.1172/JCI105336. [DOI] [PMC free article] [PubMed] [Google Scholar]