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. 1967 Feb;46(2):266–279. doi: 10.1172/JCI105529

Light and Heavy Chain Components of Gamma Globulins in Urines of Normal Persons and Patients with Agammaglobulinemia*

John H Vaughan 1,†, Ralph F Jacox 1, Barry A Gray 1,‡
PMCID: PMC297045  PMID: 4163635

Abstract

A heavy chain component of γG-globulins in normal urines has the characteristics of the proteolytic digestion product, Fc-fragment. This fragment is estimated to compose up to 15% of the gamma globulins present in urines. In contrast to urinary light chains, the urinary Fc-like fragment probably represents a catabolic component in normal γG-globulin metabolism.

The light chains of the urine constitute more than half the total gamma globulin present. The calculated κ/λ ratio of light chains averaged 1.9 in ten urines, but the ratio in individual urines varied widely to either side of this figure.

In two adult patients with agammaglobulinemia whose sera contained less than one-hundredth the normal gamma globulin levels, the urinary Fc-like fragment was absent, whereas the light chain levels were only one-tenth the average normal level. Treatment with exogenous gamma globulins resulted in normal or near normal Fc-like fragment excretion, whereas light chain excretion was only modestly affected.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERGGARD I., EDELMAN G. M. Normal counterparts to Bence-Jones proteins: free L polypeptide chains of human gamma-globulin. Proc Natl Acad Sci U S A. 1963 Mar 15;49:330–337. doi: 10.1073/pnas.49.3.330. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. COHEN S., PORTER R. B. STRUCTURE AND BIOLOGICAL ACTIVITY OF IMMUNOGLOBULINS. Adv Immunol. 1964;27:287–349. doi: 10.1016/s0065-2776(08)60710-5. [DOI] [PubMed] [Google Scholar]
  3. EDELMAN G. M., HEREMANS J. F., HEREMANS M. T., KUNKEL H. G. Immunological studies of human gamma-globulin. Relation of the precipitin lines of whole gamma-globulin to those of the fragments produced by papain. J Exp Med. 1960 Jul 1;112:203–223. doi: 10.1084/jem.112.1.203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. FLEISCHMAN J. B., PAIN R. H., PORTER R. R. Reduction of gamma-globulins. Arch Biochem Biophys. 1962 Sep;Suppl 1:174–180. [PubMed] [Google Scholar]
  5. FRANKLIN E. C., LOWENSTEIN J., BIGELOW B., MELTZER M. HEAVY CHAIN DISEASE- A NEW DISORDER OF SERUM GAMMA-GLOBULINS : REPORT OF THE FIRST CASE. Am J Med. 1964 Sep;37:332–350. doi: 10.1016/0002-9343(64)90191-3. [DOI] [PubMed] [Google Scholar]
  6. FRANKLIN E. C. Physicochemical and immunologic studies of gamma globulins of normal human urine. J Clin Invest. 1959 Dec;38:2159–2167. doi: 10.1172/JCI103995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. FRANKLIN E. C. STRUCTURAL STUDIES OF HUMAN 7S GAMMA-GLOBULIN (G IMMUNOGLOBULIN). FURTHER OBSERVATIONS OF A NATURALLY OCCURRING PROTEIN RELATED TO THE CRYSTALLIZABLE (FAST) FRAGMENT. J Exp Med. 1964 Nov 1;120:691–709. doi: 10.1084/jem.120.5.691. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. FREEDMAN M. H., CONNELL G. E. THE HETEROGENEITY OF GAMMA-GLOBULIN IN POST-EXERCISE URINE. Can J Biochem. 1964 Jul;42:1065–1097. doi: 10.1139/o64-117. [DOI] [PubMed] [Google Scholar]
  9. GITLIN D., KUMATE J., URRUSTI J., MORALES C. THE SELECTIVITY OF THE HUMAN PLACENTA IN THE TRANSFER OF PLASMA PROTEINS FROM MOTHER TO FETUS. J Clin Invest. 1964 Oct;43:1938–1951. doi: 10.1172/JCI105068. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. GREY H. M., KUNKEL H. G. H CHAIN SUBGROUPS OF MYELOMA PROTEINS AND NORMAL 7S GAMMA-GLOBULIN. J Exp Med. 1964 Aug 1;120:253–266. doi: 10.1084/jem.120.2.253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. HANSON L. A., TAN E. M. CHARACTERIZATION OF ANTIBODIES IN HUMAN URINE. J Clin Invest. 1965 May;44:703–715. doi: 10.1172/JCI105183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. HELMKAMP R. W., GOODLAND R. L., BALE W. F., SPAR I. L., MUTSCHLER L. E. High specific activity iodination of gamma-globulin with iodine-131 monochloride. Cancer Res. 1960 Nov;20:1495–1500. [PubMed] [Google Scholar]
  13. KORNGOLD L., VAN LEEUWEN G. The effect of the antigen's molecular weight on the curvature of the precipitin line in the Ouchterlony technic. J Immunol. 1957 Mar;78(3):172–177. [PubMed] [Google Scholar]
  14. MARTIN R. G., AMES B. N. A method for determining the sedimentation behavior of enzymes: application to protein mixtures. J Biol Chem. 1961 May;236:1372–1379. [PubMed] [Google Scholar]
  15. NEFF J. C., BECKER E. L. Antigen-antibody reactions in agar. III. Rate of change of band migration with antigen concentration. J Immunol. 1957 Jan;78(1):5–10. [PubMed] [Google Scholar]
  16. OSSERMAN E. F., TAKATSUKI K. PLASMA CELL MYELOMA: GAMMA GLOBULIN SYNTHESIS AND STRUCTURE. A REVIEW OF BIOCHEMICAL AND CLINICAL DATA, WITH THE DESCRIPTION OF A NEWLY-RECOGNIZED AND RELATED SYNDROME, "H-GAMMA-2-CHAIN (FRANKLIN'S) DISEASE. Medicine (Baltimore) 1963 Nov;42:357–384. [PubMed] [Google Scholar]
  17. OUCHTERLONY O. Diffusion-in-gel methods for immunological analysis. Prog Allergy. 1958;5:1–78. [PubMed] [Google Scholar]
  18. Poulik M. D. F'c fragment of immunoglobulins. Nature. 1966 Apr 9;210(5032):133–134. doi: 10.1038/210133a0. [DOI] [PubMed] [Google Scholar]
  19. REMINGTON J. S., FINLAND M. Precipitating antibody in normal human urine. Proc Soc Exp Biol Med. 1961 Aug-Sep;107:765–770. doi: 10.3181/00379727-107-26748. [DOI] [PubMed] [Google Scholar]
  20. REMINGTON J. S., MERLER E., LERNER A. M., GITLIN D., FINLAND M. Antibodies of low molecular weight in normal human urine. Nature. 1962 Apr 28;194:407–408. doi: 10.1038/194407a0. [DOI] [PubMed] [Google Scholar]
  21. RIGAS D. A., HELLER C. G. The amount and nature of urinary proteins in normal human subjects. J Clin Invest. 1951 Aug;30(8):853–861. doi: 10.1172/JCI102500. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. ROWE D. S., SOOTHILL J. F. Serum proteins in normal urine. Clin Sci. 1961 Aug;21:75–85. [PubMed] [Google Scholar]
  23. RUBINSTEIN H. M. Quantitative antigen analysis by the Oudin method. J Immunol. 1954 Nov;73(5):322–330. [PubMed] [Google Scholar]
  24. SEIJFFERS M. J., SEGAL R. L., MILLER L. L. SEPARATION OF PEPSINOGEN 2 AND PEPSINOGEN 3 FROM HUMAN URINE. Am J Physiol. 1964 May;206:1106–1110. doi: 10.1152/ajplegacy.1964.206.5.1106. [DOI] [PubMed] [Google Scholar]
  25. SOLOMON A., WALDMANN T. A., FAHEY J. L., MCFARLANE A. S. METABOLISM OF BENCE JONES PROTEINS. J Clin Invest. 1964 Jan;43:103–117. doi: 10.1172/JCI104884. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. SPIEGELBERG H. L., WEIGLE W. O. THE CATABOLISM OF HOMOLOGOUS AND HETEROLOGOUS 7S GAMMA GLOBULIN FRAGMENTS. J Exp Med. 1965 Mar 1;121:323–338. doi: 10.1084/jem.121.3.323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. STEVENSON G. T. Detection in normal urine of protein resembling Bence Jones protein. J Clin Invest. 1960 Jul;39:1192–1200. doi: 10.1172/JCI104134. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. STEVENSON G. T. Further studies of the gamma-related proteins of normal urine. J Clin Invest. 1962 May;41:1190–1198. doi: 10.1172/JCI104572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. TERRY W. D., FAHEY J. L. SUBCLASSES OF HUMAN GAMMA-2-GLOBULIN BASED ON DIFFERENCES IN THE HEAVY POLYPEPTIDE CHAINS. Science. 1964 Oct 16;146(3642):400–401. doi: 10.1126/science.146.3642.400. [DOI] [PubMed] [Google Scholar]
  30. Turner M. W., Rowe D. S. A naturally occurring fragment related to the heavy chains of immunoglobulin G in normal human urine. Nature. 1966 Apr 9;210(5032):130–132. doi: 10.1038/210130a0. [DOI] [PubMed] [Google Scholar]
  31. WEBB T., ROSE B., SEHON A. H. Biocolloids in normal human urine. I. Amount and electrophoretic characteristics. Can J Biochem Physiol. 1958 Nov;36(11):1159–1166. [PubMed] [Google Scholar]
  32. WEBB T., ROSE B., SEHON A. H. Biocolloids in normal human urine. II. Physicochemical and immunochemical characteristics. Can J Biochem Physiol. 1958 Nov;36(11):1167–1175. [PubMed] [Google Scholar]

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