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. 1967 Mar;46(3):357–368. doi: 10.1172/JCI105537

Peroxidative Hemolysis of Red Blood Cells from Patients with Abetalipoproteinemia (Acanthocytosis)*

James T Dodge 1,2,3,4,†, Gerald Cohen 1,2,3,4, Herbert J Kayden 1,2,3,4,‡, Gerald B Phillips 1,2,3,4,§
PMCID: PMC297056  PMID: 6023771

Abstract

The effect of peroxidative stress on tissue was studied by exposure of red blood cells (RBC) from patients with abetalipoproteinemia to minute amounts of H2O2in vitro. Red blood cells from untreated patients showed a marked sensitivity to H2O2, as evidenced by hemolysis and lipid peroxidation (peroxidative hemolysis).

The appearance of lipid peroxidation products in sensitive cells after exposure to H2O2 was indicated by 1) increases in the 2-thiobarbituric acid (TBA) reaction of trichloroacetic acid extracts, 2) increases in ultraviolet light absorbency of lipid extracts, and 3) decreases in polyunsaturated fatty acids. These changes were accompanied by a decrease in phosphatidyl ethanolamine and phosphatidyl serine in the RBC lipid extract. Similar lipid changes on exposure to H2O2 were observed in the RBC from vitamin E-deficient rats.

Treatment of the patients with d-α-tocopherol polyethylene glycol succinate by mouth, or addition of dl-α-tocopherol to the incubation medium protected the RBC from peroxidative hemolysis. Tocopherol appears to provide a primary biologic defense against peroxidative hemolysis.

The presence of nitrite or carbon monoxide, which produced methemoglobin and carboxyhemoglobin, respectively, inhibited peroxidative changes, suggesting a catalytic role for oxy- or deoxyhemoglobin.

Substances that prevented lipid peroxidation also prevented hemolysis; in addition, lipid peroxidation appeared to precede hemolysis. These observations suggested that hemolysis was a consequence of lipid peroxidation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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