Abstract
Double diffusion in agarose was employed for the characterization of a soluble nucleoprotein antigen that gave precipitin reactions with sera from patients with systemic lupus erythematosus. The soluble antigen that was isolated from calf thymus nuclei was demonstrated by enzyme degradation and ultracentrifugation studies, and by immunologic analysis, to be a complex of deoxyribonucleic acid and a moiety susceptible to proteolytic digestion. Antibody to soluble nucleoprotein did not react with the DNA portion of the complex released after proteolytic digestion or with purified calf thymus DNA. Immunologic reaction of identity was obtained between the soluble nucleoprotein antigen and synthesized DNA-histone complex, suggesting that the protein moiety of soluble nucleoprotein might in part be composed of histone. Antibody to soluble nucleoprotein was present in 51% of systemic lupus erythematosus sera examined and was more common than antibody to deoxyribonucleic acid.
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- ALLFREY V. G., LITTAU V. C., MIRSKY A. E. METHODS FOR THE PURIFICATION OF THYMUS NUCLEI AND THEIR APPLICATION TO STUDIES OF NUCLEAR PROTEIN SYNTHESIS. J Cell Biol. 1964 May;21:213–231. doi: 10.1083/jcb.21.2.213. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ANDERSON J. R., GRAY K. G., BECK J. S., BUCHANAN W. W., McELHINNEY A. J. Precipitating auto-antibodies in the connective tissue diseases. Ann Rheum Dis. 1962 Dec;21:360–369. doi: 10.1136/ard.21.4.360. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ASHERSON G. L. Antibodies against nuclear and cytoplasmic cell constituents in systemic lupus erythematosus and other diseases. Br J Exp Pathol. 1959 Jun;40(3):209–215. [PMC free article] [PubMed] [Google Scholar]
- ATCHLEY W. A. An immunoelectrophoretic analysis of antigens reacting with lupus serum. Arthritis Rheum. 1961 Oct;4:471–479. doi: 10.1002/art.1780040504. [DOI] [PubMed] [Google Scholar]
- BECK J. S. Variations in the morphological patterns of "autoimmune" nuclear fluorescence. Lancet. 1961 Jun 3;1(7188):1203–1205. doi: 10.1016/s0140-6736(61)91944-4. [DOI] [PubMed] [Google Scholar]
- CASALS S. P., FRIOU G. J., TEAGUE P. O. SPECIFIC NUCLEAR REACTION PATTERN OF ANTIBODY TO DNA IN LUPUS ERYTHEMATOSUS SERA. J Lab Clin Med. 1963 Oct;62:625–631. [PubMed] [Google Scholar]
- CEPPELLINI R., POLLI E., CELADA F. A DNA-reacting factor in serum of a patient with lupus erythematosus diffusus. Proc Soc Exp Biol Med. 1957 Dec;96(3):572–574. doi: 10.3181/00379727-96-23544. [DOI] [PubMed] [Google Scholar]
- CHIH R., HUANG C., BONNER J., MURRAY K. PHYSICAL AND BIOLOGICAL PROPERTIES OF SOLUBLE NUCLEOHISTONES. J Mol Biol. 1964 Jan;8:54–64. doi: 10.1016/s0022-2836(64)80148-0. [DOI] [PubMed] [Google Scholar]
- DEICHER H. R., HOLMAN H. R., KUNKEL H. G. Anti-cytoplasmic factors in the sera of patients with systemic lupus erythematosus and certain other diseases. Arthritis Rheum. 1960 Feb;3:1–15. doi: 10.1002/art.1780030102. [DOI] [PubMed] [Google Scholar]
- DEICHER H. R., HOLMAN H. R., KUNKEL H. G. The precipitin reaction between DNA and a serum factor in systemic lupus erythematosus. J Exp Med. 1959 Jan 1;109(1):97–114. doi: 10.1084/jem.109.1.97. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Frenster J. H. Nuclear polyanions as de-repressors of synthesis of ribonucleic acid. Nature. 1965 May 15;206(985):680–683. doi: 10.1038/206680a0. [DOI] [PubMed] [Google Scholar]
- HOLMAN H., DEICHER H. R. The reaction of the lupus erythematosus (L.E.) cell factor with deoxyribonucleoprotein of the cell nucleus. J Clin Invest. 1959 Nov;38:2059–2072. doi: 10.1172/JCI103984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LACHMANN P. J. A two-stage indirect L.E. cell test. Immunology. 1961 Apr;4:142–152. [PMC free article] [PubMed] [Google Scholar]
- LACHMANN P. J. An attempt to characterize the lupus erythematosus cell antigen. Immunology. 1961 Apr;4:153–163. [PMC free article] [PubMed] [Google Scholar]
- LACHMANN P. J., KUNKEL H. G. Correlation of antinuclear antibodies and nuclear staining patterns. Lancet. 1961 Aug 19;2(7199):436–437. doi: 10.1016/s0140-6736(61)92523-5. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- MASTER R. W. POSSIBLE SYNTHESIS OF POLYRIBONUCLEOTIDES OF KNOWN BASE-TRIPLET SEQUENCES. Nature. 1965 Apr 3;206:93–93. doi: 10.1038/206093b0. [DOI] [PubMed] [Google Scholar]
- PEARSON C. M., CRADDOCK C. G., SIMMONS N. S. Complement fixation reactions with DNA and leukocyte material in systemic lupus erythematosus: correlation with the L. E. cell phenomenon and the clinical status. J Lab Clin Med. 1958 Oct;52(4):580–587. [PubMed] [Google Scholar]
- ROBBINS W. C., HOLMAN H. R., DEICHER H., KUNKEL H. G. Complement fixation with cell nuclei and DNA in lupus erythematosus. Proc Soc Exp Biol Med. 1957 Dec;96(3):575–579. doi: 10.3181/00379727-96-23545. [DOI] [PubMed] [Google Scholar]
- SELIGMANN M. Etudes immunologiques sur le lupus érythémateux disséminé. Rev Fr Etud Clin Biol. 1958 Jun;3(6):558–584. [PubMed] [Google Scholar]
- SELIGMANN M. Mise en évidence dans le sérum de malades atteints de lupus erythémateux disséminé d'une substance déterminant une réaction de précipitation avec l'acide désoxyribonucléique. C R Hebd Seances Acad Sci. 1957 Jul 8;245(2):243–245. [PubMed] [Google Scholar]
- Tan E. M., Kunkel H. G. Characteristics of a soluble nuclear antigen precipitating with sera of patients with systemic lupus erythematosus. J Immunol. 1966 Mar;96(3):464–471. [PubMed] [Google Scholar]
- Tan E. M., Schur P. H., Carr R. I., Kunkel H. G. Deoxybonucleic acid (DNA) and antibodies to DNA in the serum of patients with systemic lupus erythematosus. J Clin Invest. 1966 Nov;45(11):1732–1740. doi: 10.1172/JCI105479. [DOI] [PMC free article] [PubMed] [Google Scholar]
- VORLAENDER K. O., ROSS J. [The agar-gel diffusion technic in the immunological study of clinical disorders]. Klin Wochenschr. 1961 Jun 15;39:605–610. doi: 10.1007/BF01479079. [DOI] [PubMed] [Google Scholar]