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. Author manuscript; available in PMC: 2011 Dec 5.
Published in final edited form as: Virology. 2010 Dec 5;408(1):119–127. doi: 10.1016/j.virol.2010.09.005

Fig. 3. Expression of Nef2 mRNA in infected podocytes and HIV-1 virions.

Fig. 3

(A) Differentiated podocytes were infected with NL4-3 ΔEnv virions pseudotyped with HXB2 or VSV-G envelopes. At indicated times, cellular RNA was isolated, reverse transcribed and analyzed by real-time RT-PCR. Expression of Nef2 mRNA was normalized to GAPDH and the level of Nef2 mRNA at 3 h post-infection with HXB2 pseudotyped HIV-1 was set to 100%. (B) Relative expression of genomic unspliced (usRNA) RNA, multiply spliced RNA (msRNA) and Nef2 mRNA in AD8 virions were quantitated and normalized against 7SL RNA (Onafuwa-Nuga, Telesnitsky, and King, 2006). Expression of virion usRNA was set to 100%.