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. Author manuscript; available in PMC: 2011 Dec 5.
Published in final edited form as: Virology. 2010 Dec 5;408(1):119–127. doi: 10.1016/j.virol.2010.09.005

Fig. 5. HIV-1 released from infected podocytes is infectious.

Fig. 5

(A) AD8 added to differentiated AB8/13 podocytes (AD8 input) and released from podocytes between 6 and 24 h post-infection (AD8 recovered) were tittered and the same amount of viral inoculum (RT assay standardized) was added in triplicate to TZM-bl indicator cells in a 12-well plate. Three dilutions of viral inoculum were applied: 105, 104, and 103 RT cpm. After 2 days, cells were fixed and stained for β-galactosidase and positive syncytia were counted (B). Viral inoculum (105 RT cpm) applied to indicator cells is shown. Control, uninfected TZM-bl cells. (B) Percent infectivity is presented relative to infectivity of input virus (100%). Error bars, means ± SDs of triplicate samples from one experiment.