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. 1968 May;47(5):1083–1095. doi: 10.1172/JCI105798

Characteristics of the RNA synthesized in vitro by the normal human small lymphocyte and the changes induced by phytohemagglutinin stimulation

Umberto L Torelli 1, Patrick H Henry 1, Sherman M Weissman 1
PMCID: PMC297261  PMID: 5645854

Abstract

These studies demonstrate that the circulating human small lymphocyte synthesizes ribonucleic acid (RNA) of high molecular weight which is not primarily a ribosomal precursor and which is, in part, complementary to human deoxyribonucleic acid (DNA). The stimulation of these lymphocytes by PHA brings a cell population in which few ribosomes are synthesized to a functional condition in which a large amount of these particles are produced. This increase in the synthesis of ribosomal RNA is one of the earliest and most relevant effects of PHA on the RNA metabolism of small lymphocytes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Attardi G., Parnas H., Hwang M. I., Attardi B. Giant-size rapidly labeled nuclear ribonucleic acid and cytoplasmic messenger ribonucleic acid in immature duck erythrocytes. J Mol Biol. 1966 Sep;20(1):145–182. doi: 10.1016/0022-2836(66)90123-9. [DOI] [PubMed] [Google Scholar]
  2. BOND V. P., FLIEDNER T. M., CRONKITE E. P., RUBINI J. R., BRECHER G., SCHORK P. K. Proliferative potentials of bone marrow and blood cells studied by in vitro uptake of H3-thymidine. Acta Haematol. 1959 Jan;21(1):1–15. doi: 10.1159/000205651. [DOI] [PubMed] [Google Scholar]
  3. Birnboim H. C., Pène J. J., Darnell J. E. Studies on HeLa cell nuclear DNA-like RNA by RNA-DNA hybridization. Proc Natl Acad Sci U S A. 1967 Jul;58(1):320–327. doi: 10.1073/pnas.58.1.320. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brown G. M., Attardi G. Methylation of nucleic acids in HeLa cells. Biochem Biophys Res Commun. 1965 Jul 26;20(3):298–302. doi: 10.1016/0006-291x(65)90363-3. [DOI] [PubMed] [Google Scholar]
  5. Burdon R. H. Methylation of nucleic acids in Krebs II ascites tumour cells. Nature. 1966 May 21;210(5038):797–799. doi: 10.1038/210797a0. [DOI] [PubMed] [Google Scholar]
  6. CARSTAIRS K. The human small lymphocyte: its possible pluripotential quality. Lancet. 1962 Apr 21;1(7234):829–832. doi: 10.1016/s0140-6736(62)91841-x. [DOI] [PubMed] [Google Scholar]
  7. COOPER H. L., RUBIN A. D. RNA METABOLISM IN LYMPHOCYTES STIMULATED BY PHYTOHEMAGGLUTININ: INITIAL RESPONSES TO PHYTOHEMAGGLUTININ. Blood. 1965 Jun;25:1014–1027. [PubMed] [Google Scholar]
  8. Church R. B., McCarthy B. J. Ribonucleic acid synthesis in regenerating and embryonic liver. I. The synthesis of new species of RNA during regeneration of mouse liver after partial hepatectomy. J Mol Biol. 1967 Feb 14;23(3):459–475. doi: 10.1016/s0022-2836(67)80118-9. [DOI] [PubMed] [Google Scholar]
  9. Cline M. J. Isolation and characterization of RNA from human leukocytes. J Lab Clin Med. 1966 Jul;68(1):33–46. [PubMed] [Google Scholar]
  10. Cooper H. L. Ribonucleic acid metabolism in lymphocytes stimulated by phytohemagglutinin. II. Rapidly synthesized ribonucleic acid and te production of ribosomal ribonucleic acid. J Biol Chem. 1968 Jan 10;243(1):34–43. [PubMed] [Google Scholar]
  11. DAVIDSON J. D., FEIGELSON P. Practical aspects of internal-sample liquid-scintillation counting. Int J Appl Radiat Isot. 1957 Apr;2(1):1–18. doi: 10.1016/0020-708x(57)90021-2. [DOI] [PubMed] [Google Scholar]
  12. DUNN D. B. Additional components in ribonucleic acid of rat-liver fractions. Biochim Biophys Acta. 1959 Jul;34:286–288. doi: 10.1016/0006-3002(59)90274-4. [DOI] [PubMed] [Google Scholar]
  13. EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
  14. GOWANS J. L. THE ROLE OF LYMPHOCYTES IN THE DESTRUCTION OF HOMOGRAFTS. Br Med Bull. 1965 May;21:106–110. doi: 10.1093/oxfordjournals.bmb.a070376. [DOI] [PubMed] [Google Scholar]
  15. GREENWALT T. J., GAJEWSKI M., McKENNA J. L. A new method for preparing buffy coat-poor blood. Transfusion. 1962 Jul-Aug;2:221–229. doi: 10.1111/j.1537-2995.1962.tb00228.x. [DOI] [PubMed] [Google Scholar]
  16. Henry P., Reich P., Karon M., Weissman S. M. Characteristics of RNA synthesized in vitro by lymphocytes of chronic lymphocytic leukemia. J Lab Clin Med. 1967 Jan;69(1):47–61. [PubMed] [Google Scholar]
  17. INMAN D. R., COOPER E. H. ELECTRON MICROSCOPY OF HUMAN LYMPHOCYTES STIMULATED BY PHYTOHAEMAGGLUTININ. J Cell Biol. 1963 Nov;19:441–445. doi: 10.1083/jcb.19.2.441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. KIRBY K. S. ISOLATION AND CHARACTERIZATION OF RIBOSOMAL RIBONUCLEIC ACID. Biochem J. 1965 Jul;96:266–269. doi: 10.1042/bj0960266. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. LITTLE J. R., BRECHER G., BRADLEY T. R., ROSE S. Determination of lymphocyte turnover by continuous infusion of H3 thymidine. Blood. 1962 Feb;19:236–242. [PubMed] [Google Scholar]
  20. Martin M. A., Hoyer B. H. Thermal stabilities and species specificities of reannealed animal deoxyribonucleic acids. Biochemistry. 1966 Aug;5(8):2706–2713. doi: 10.1021/bi00872a030. [DOI] [PubMed] [Google Scholar]
  21. McCarthy B. J. The evolution of base sequences in polynucleotides. Prog Nucleic Acid Res Mol Biol. 1965;4:129–160. doi: 10.1016/s0079-6603(08)60786-2. [DOI] [PubMed] [Google Scholar]
  22. Merits I., Schulze W., Overby L. R. Ribosomal ribonucleic acid sites in chicken deoxyribonucleic acid. Arch Biochem Biophys. 1966 Jul;115(1):197–205. doi: 10.1016/s0003-9861(66)81057-3. [DOI] [PubMed] [Google Scholar]
  23. Moore P. B. Methylation of messenger RNA in Escherichia coli. J Mol Biol. 1966 Jun;18(1):38–47. doi: 10.1016/s0022-2836(66)80074-8. [DOI] [PubMed] [Google Scholar]
  24. Mueller G. C., Mahieu M. L. Induction of ribonucleic acid synthesis in human leucocytes by phytohemagglutinin. Biochim Biophys Acta. 1966 Jan 18;114(1):100–107. doi: 10.1016/0005-2787(66)90257-7. [DOI] [PubMed] [Google Scholar]
  25. NOWELL P. C. Phytohemagglutinin: an initiator of mitosis in cultures of normal human leukocytes. Cancer Res. 1960 May;20:462–466. [PubMed] [Google Scholar]
  26. NYGAARD A. P., HALL B. D. A method for the detection of RNA-DNA complexes. Biochem Biophys Res Commun. 1963 Jul 18;12:98–104. doi: 10.1016/0006-291x(63)90242-0. [DOI] [PubMed] [Google Scholar]
  27. OPPENHEIM J. J., WHANG J., FREI E., 3rd IMMUNOLOGIC AND CYTOGENETIC STUDIES OF CHRONIC LYMPHOCYTIC LEUKEMIC CELLS. Blood. 1965 Aug;26:121–132. [PubMed] [Google Scholar]
  28. OTTESEN J. On the age of human white cells in peripheral blood. Acta Physiol Scand. 1954 Oct 20;32(1):75–93. doi: 10.1111/j.1748-1716.1954.tb01157.x. [DOI] [PubMed] [Google Scholar]
  29. PETERMANN M. L., PAVLOVEC A. STUDIES ON RIBONUCLEIC ACID FROM RAT LIVER RIBOSOMES. J Biol Chem. 1963 Nov;238:3717–3724. [PubMed] [Google Scholar]
  30. Penman S. RNA metabolism in the HeLa cell nucleus. J Mol Biol. 1966 May;17(1):117–130. doi: 10.1016/s0022-2836(66)80098-0. [DOI] [PubMed] [Google Scholar]
  31. Pogo B. G., Allfrey V. G., Mirsky A. E. RNA synthesis and histone acetylation during the course of gene activation in lymphocytes. Proc Natl Acad Sci U S A. 1966 Apr;55(4):805–812. doi: 10.1073/pnas.55.4.805. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. QUAGLINO D., HAYHOE F. G., FLEMANS R. J. Cytochemical observations on the effect of phytohaemagglutinin in short-term tissue cultures. Nature. 1962 Oct 27;196:338–340. doi: 10.1038/196338a0. [DOI] [PubMed] [Google Scholar]
  33. Rubin A. D., Cooper H. L. Evolving patterns of RNA metabolism during transition from resting state to active growth in lymphocytes stimulated by phytohemagglutinin. Proc Natl Acad Sci U S A. 1965 Aug;54(2):469–476. doi: 10.1073/pnas.54.2.469. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. SCHERRER K., DARNELL J. E. Sedimentation characteristics of rapidly labelled RNA from HeLa cells. Biochem Biophys Res Commun. 1962 Jun 4;7:486–490. doi: 10.1016/0006-291x(62)90341-8. [DOI] [PubMed] [Google Scholar]
  35. SCHERRER K., LATHAM H., DARNELL J. E. Demonstration of an unstable RNA and of a precursor to ribosomal RNA in HeLa cells. Proc Natl Acad Sci U S A. 1963 Feb 15;49:240–248. doi: 10.1073/pnas.49.2.240. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. SMITH J. D., DUNN D. B. An additional sugar component of ribonucleic acids. Biochim Biophys Acta. 1959 Feb;31(2):573–575. doi: 10.1016/0006-3002(59)90045-9. [DOI] [PubMed] [Google Scholar]
  37. STARR J. L., FEFFERMAN R. THE OCCURRENCE OF METHYLATED BASES IN RIBOSOMAL RIBONUCLEIC ACID OF ESCHERICHIA COLI K12 W-6. J Biol Chem. 1964 Oct;239:3457–3461. [PubMed] [Google Scholar]
  38. Saponara A. G., Enger M. D. Incorporation of [3-H]uridine and [M3-14-C]methionine into Chinese-hamster cell ribonucleic acid. Biochim Biophys Acta. 1966 Jun 22;119(3):492–500. [PubMed] [Google Scholar]
  39. Scherrer K., Marcaud L., Zajdela F., London I. M., Gros F. Patterns of RNA metabolism in a differentiated cell: a rapidly labeled, unstable 60S RNA with messenger properties in duck erythroblasts. Proc Natl Acad Sci U S A. 1966 Nov;56(5):1571–1578. doi: 10.1073/pnas.56.5.1571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. TORELLI U., GROSSI G., ARTUSI T., EMILIA G. RNA AND PROTEIN SYNTHESIS IN NORMAL PERIPHERAL MONONUCLEAR LEUKOCYTES. A RADIOAUTOGRAPHIC STUDY. Acta Haematol. 1963;30:129–137. doi: 10.1159/000208116. [DOI] [PubMed] [Google Scholar]
  41. Tata J. R. The formation and distribution of ribosomes during hormone-induced growth and development. Biochem J. 1967 Jul;104(1):1–16. doi: 10.1042/bj1040001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. Warner J. R., Soeiro R., Birnboim H. C., Girard M., Darnell J. E. Rapidly labeled HeLa cell nuclear RNA. I. Identification by zone sedimentation of a heterogeneous fraction separate from ribosomal precursor RNA. J Mol Biol. 1966 Aug;19(2):349–361. doi: 10.1016/s0022-2836(66)80009-8. [DOI] [PubMed] [Google Scholar]
  43. Zimmerman E. F., Holler B. W. Methylation of 45 s ribosomal RNA precursor in HeLa cells. J Mol Biol. 1967 Jan 28;23(2):149–161. doi: 10.1016/s0022-2836(67)80023-8. [DOI] [PubMed] [Google Scholar]

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