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. Author manuscript; available in PMC: 2011 Oct 19.
Published in final edited form as: Biochemistry. 2010 Oct 19;49(41):8818–8830. doi: 10.1021/bi100557v

Figure 4.

Figure 4

Native gel electrophoresis of 1 nM 32P labeled 18 bp DNA and 1 µM SgrAI dimer and varied concentrations of unlabeled DNA. A. 1 nM 32P labeled primary site (18-1) with added unlabeled PCP (10, 30, 60, 100, 200, 300, 400, 500, 600, 1000 nM). B. 1 nM 32P labeled secondary site (18-2) with added unlabeled PCP (10, 30, 60, 100, 200, 300, 400, 500, 600, 1000 nM). C. Plot of percent of DNA (1 nM 32P-18-1) bound species with 1 µM SgrAI found in HMWS vs. concentration of added unlabeled PCP (open circles), 40-1 (open boxes), 18-1 (filled circles), or 40-2 (filled boxes).