Skip to main content
NCBI home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1969 Dec;48(12):2293–2299. doi: 10.1172/JCI106195

Inheritance of human α1-acid glycoprotein (orosomucoid) variants

A Myron Johnson 1,2,3,4, Karl Schmid 1,2,3,4, Chester A Alper 1,2,3,4
PMCID: PMC297486  PMID: 4982230

Abstract

Although variants of sialic acid-free α1-acid glycoprotein have been described in human beings, the mode of inheritance of these types has not been reported previously. With the use of a new technique of immunofixation after agarose gel electrophoresis of neuraminidase-treated whole serum, the present study demonstrates that the types of α1-acid glycoprotein variants in family members are consistent with inheritance as autosomal traits with codominant expression. Gene frequencies have been determined for several ethnic groups. Of a total of 11 maternal-cord serum pairs, seven were discordant types, indicating that the fetus synthesizes α1-acid glycoprotein and confirming a previous report that there is no significant transplacental passage of this protein.

Full text

PDF
2293

Images in this article

2294Image
on p.2294
2294Image
on p.2294
2294Image
on p.2294
2295Image
on p.2295

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alper C. A., Johnson A. M. Immunofixation electrophoresis: a technique for the study of protein polymorphism. Vox Sang. 1969 Nov;17(5):445–452. doi: 10.1111/j.1423-0410.1969.tb00414.x. [DOI] [PubMed] [Google Scholar]
  2. Alper C. A., Propp R. P. Genetic polymorphism of the third component of human complement (C'3). J Clin Invest. 1968 Sep;47(9):2181–2191. doi: 10.1172/JCI105904. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Axelsson U., Laurell C. B. Hereditary variants of serum alpha-1-antitrypsin. Am J Hum Genet. 1965 Nov;17(6):466–472. [PMC free article] [PubMed] [Google Scholar]
  4. CLEVE H., KIRK R. L., PARKER W. C., BEARN A. G., SCHACHT L. E., KLEINMAN H., HORSFALL W. R. TWO GENETIC VARIANTS OF THE GROUP-SPECIFIC COMPONENT OF HUMAN SERUM: GC CHIPPEWA AND GC ABORIGINE. Am J Hum Genet. 1963 Dec;15:368–379. [PMC free article] [PubMed] [Google Scholar]
  5. GITLIN D., KUMATE J., URRUSTI J., MORALES C. THE SELECTIVITY OF THE HUMAN PLACENTA IN THE TRANSFER OF PLASMA PROTEINS FROM MOTHER TO FETUS. J Clin Invest. 1964 Oct;43:1938–1951. doi: 10.1172/JCI105068. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HARRIS H., PENINGTON D. G., ROBSON E. B., SCRIVER C. R. A further genetically determined transferrin variant in man. Ann Hum Genet. 1960 Dec;24:327–332. doi: 10.1111/j.1469-1809.1960.tb01745.x. [DOI] [PubMed] [Google Scholar]
  7. HEIMBURGER N., HEIDE K., HAUPT H., SCHULTZE H. E. BAUSTEINANALYSEN VON HUMANSERUMPROTEINEN. Clin Chim Acta. 1964 Oct;10:293–307. doi: 10.1016/0009-8981(64)90059-2. [DOI] [PubMed] [Google Scholar]
  8. Hunziker K., Schmid K. A brief method for the identification of the alpha-1-acid glycoprotein variants. Anal Biochem. 1967 Sep;20(3):495–501. doi: 10.1016/0003-2697(67)90294-1. [DOI] [PubMed] [Google Scholar]
  9. LAURELL C. B. ANTIGEN-ANTIBODY CROSSED ELECTROPHORESIS. Anal Biochem. 1965 Feb;10:358–361. doi: 10.1016/0003-2697(65)90278-2. [DOI] [PubMed] [Google Scholar]
  10. Laurell C. B., Niléhn J. E. A new type of inherited serum albumin anomaly. J Clin Invest. 1966 Dec;45(12):1935–1945. doi: 10.1172/JCI105498. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Laurell C. B. Orosomucoid and alpha 1-antitrypsin in maternal and fetal sera at parturition. Scand J Clin Lab Invest. 1968;21(2):136–138. doi: 10.3109/00365516809084275. [DOI] [PubMed] [Google Scholar]
  12. Laurell C. B. Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Anal Biochem. 1966 Apr;15(1):45–52. doi: 10.1016/0003-2697(66)90246-6. [DOI] [PubMed] [Google Scholar]
  13. PARKER W. C., BEARN A. G. Control gene mutations in the human haptoglobin system. Nature. 1963 Apr 6;198:107–108. doi: 10.1038/198107a0. [DOI] [PubMed] [Google Scholar]
  14. Poulik M. D. Heterogeneity and structure of ceruloplasmin. Ann N Y Acad Sci. 1968 Jun 14;151(1):476–501. doi: 10.1111/j.1749-6632.1968.tb11909.x. [DOI] [PubMed] [Google Scholar]
  15. Propp R. P., Alper C. A. C'3 synthesis in the human fetus and lack of transplacental passage. Science. 1968 Nov 8;162(3854):672–673. doi: 10.1126/science.162.3854.672. [DOI] [PubMed] [Google Scholar]
  16. Reinskou T. A new variant in the Gc system. Acta Genet Stat Med. 1965;15(3):248–255. doi: 10.1159/000151917. [DOI] [PubMed] [Google Scholar]
  17. SCHEIDEGGER J. J. Une micro-méthode de l'immuno-electrophorèse. Int Arch Allergy Appl Immunol. 1955;7(2):103–110. [PubMed] [Google Scholar]
  18. SCHMID K., BINETTE J. P., TOKITA K., MOROZ L., YOSHIZAKI H. THE POLYMORPHIC FORMS OF ALPHA-1-ACID GLYCOPROTEIN OF NORMAL CAUCASIAN INDIVIDUALS. J Clin Invest. 1964 Dec;43:2347–2352. doi: 10.1172/JCI105108. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. SCHMID K., TOKITA K., YOSHIZAKI H. THE ALPHA-1-ACID GLYCOPROTEIN VARIANTS OF NORMAL CAUCASIAN AND JAPANESE INDIVIDUALS. J Clin Invest. 1965 Aug;44:1394–1401. doi: 10.1172/JCI105244. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. SMITHIES O. An improved procedure for starch-gel electrophoresis: further variations in the serum proteins of normal individuals. Biochem J. 1959 Mar;71(3):585–587. doi: 10.1042/bj0710585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Schmid K., Field R. A., Yoshizaki H. Constancy of alpha-1-acid glycoprotein variants of Caucasian patients under conditions of severe stress. J Med Genet. 1968 Mar;5(1):36–39. doi: 10.1136/jmg.5.1.36. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Schmid K., Okuyama T., Kaufmann H. Isolation and chemical composition of the human plasma alpha-1-acid glycoprotein variants. Biochim Biophys Acta. 1968 Apr 9;154(3):565–572. doi: 10.1016/0005-2795(68)90017-2. [DOI] [PubMed] [Google Scholar]
  23. Schmid K., Polis A., Hunziker K., Fricke R., Yayoshi M. Partial characterization of the sialic acid-free forms of alpha-1-acid glycoprotein from human plasma. Biochem J. 1967 Aug;104(2):361–368. doi: 10.1042/bj1040361. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Shreffler D. C., Brewer G. J., Gall J. C., Honeyman M. S. Electrophoretic variation in human serum ceruloplasmin: a new genetic polymorphism. Biochem Genet. 1967 Sep;1(2):101–115. doi: 10.1007/BF00486512. [DOI] [PubMed] [Google Scholar]
  25. Störiko K. Normal values for 23 different human plasma proteins determined by single radial immunodiffusion. Blut. 1968 Jan;16(4):200–208. doi: 10.1007/BF01631669. [DOI] [PubMed] [Google Scholar]
  26. Tokita K., Burke J. F., Yoshizaki H., Fischer S., Schmid K. The constancy of the alpha-1-acid glycoprotein variants of normal adults under conditions of severe stress. J Clin Invest. 1966 Oct;45(10):1624–1630. doi: 10.1172/JCI105469. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Uriel J. Méthode d'électrophorèse dans des gels d'acrylamide-agarose. Bull Soc Chim Biol (Paris) 1966;48(8):969–982. [PubMed] [Google Scholar]
  28. WEIMER H. E., MEHL J. W., WINZLER R. J. Studies on the mucoproteins of human plasma. V. Isolation and characterization of a homogeneous mucoprotein. J Biol Chem. 1950 Aug;185(2):561–568. [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES