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The silenced alleles lack steady-state p1 transcripts. RT–PCR was performed on total RNA extracted from cob glumes of mutant and wild-type genotypes at the 21-DAP stage. Two regions of P1-wr cDNA were amplified with p1-specific primer pairs: A, PRTEP5-8 + PRTEP3-13; B, PRTEP5-8 + PRTEP3-12 (see materials and methods for details). (Bottom) The same samples amplified with gpc1-specific primers as positive controls.
