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. 2010 Nov;186(3):843–855. doi: 10.1534/genetics.110.117929

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Figure 6.— — Reactivation of the silenced P1-wr epialleles in the presence of Ufo1. (A and B) Ufo1-induced gain of cob glume pigmentation in F₁ progenies of a cross of p1-ww; Ufo1 with p1-ww:DP3 (A) and p1-ww:DP8 (B) plants. Cob glume and kernel phenotypes of six representative ears of each cross are shown. (C) DNA gel blots showing methylation modifications of the P1-wr epialleles in the presence of Ufo1. Gel blots carrying HpaII-digested leaf genomic DNA of F₁ progeny plants of (p1-ww:DP3 × p1-ww; Ufo1) and (p1-ww:DP8 × p1-ww; Ufo1) were sequentially hybridized with probe fragments 15 (top panels) and 8B (bottom panels). Sizes and positions of bands hybridizing with the two probes are shown on the left, and molecular weight markers in kilobase pairs are shown on the right. Arrows at the bottom of the panels indicate the plants that showed gain of cob pigmentation in the presence of Ufo1.