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Analysis of ERCs and replicative life span, of histone mutants. (A) Analysis of rDNA and ERCs isolated from wild-type or histone mutant strains. Probe was amplified from genomic DNA specific to 25S rDNA. ERCs are indicated by a bracket. The arrow indicates sheared genome rDNA. (B) Replicative life span analysis was performed in either wild-type or sir2Δ or histone mutant strains. The strains used for replicative life span are the same as that in A.
