Abstract
We analyzed a t(1;14)(p32;q11) chromosomal translocation in a human lymphohemopoietic stem cell line derived from a patient with acute T-lymphoblastic leukemia. The chromosomal joining on the 1p+ chromosome occurred at the T-cell receptor delta diversity (D delta 2) segment, and the reciprocal chromosomal joining on the 14q- chromosome occurred at the T-cell delta diversity segment D delta 1. The involvement of delta diversity segments at the translocation junctions suggests that the translocation occurred during an attempt at D delta 1-D delta 2 joining in a stem cell. The segment of chromosome 1 at band p32, adjacent to the chromosomal breakpoint, encodes a transcriptional unit designated TCL5 (T-cell leukemia/lymphoma 5). The differential expression of the TCL5 RNA transcripts in this lymphohemopoietic stem cell line relative to several other T- and B-cell lines suggests that TCL5 gene expression is an integral event in the pathogenesis of the T-cell leukemia. Rearrangement of the TCL5 locus in a human melanoma cell line carrying a del(1p32) further implies that the TCL5 gene may play a role in malignant transformation.
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Selected References
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- Balaban G. B., Herlyn M., Clark W. H., Jr, Nowell P. C. Karyotypic evolution in human malignant melanoma. Cancer Genet Cytogenet. 1986 Jan 1;19(1-2):113–122. doi: 10.1016/0165-4608(86)90378-x. [DOI] [PubMed] [Google Scholar]
- Begley C. G., Aplan P. D., Davey M. P., Nakahara K., Tchorz K., Kurtzberg J., Hershfield M. S., Haynes B. F., Cohen D. I., Waldmann T. A. Chromosomal translocation in a human leukemic stem-cell line disrupts the T-cell antigen receptor delta-chain diversity region and results in a previously unreported fusion transcript. Proc Natl Acad Sci U S A. 1989 Mar;86(6):2031–2035. doi: 10.1073/pnas.86.6.2031. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Croce C. M., Isobe M., Palumbo A., Puck J., Ming J., Tweardy D., Erikson J., Davis M., Rovera G. Gene for alpha-chain of human T-cell receptor: location on chromosome 14 region involved in T-cell neoplasms. Science. 1985 Mar 1;227(4690):1044–1047. doi: 10.1126/science.3919442. [DOI] [PubMed] [Google Scholar]
- Croce C. M., Nowell P. C. Molecular basis of human B cell neoplasia. Blood. 1985 Jan;65(1):1–7. [PubMed] [Google Scholar]
- Dürst M., Croce C. M., Gissmann L., Schwarz E., Huebner K. Papillomavirus sequences integrate near cellular oncogenes in some cervical carcinomas. Proc Natl Acad Sci U S A. 1987 Feb;84(4):1070–1074. doi: 10.1073/pnas.84.4.1070. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Erickson J. M., Rushford C. L., Dorney D. J., Wilson G. N., Schmickel R. D. Structure and variation of human ribosomal DNA: molecular analysis of cloned fragments. Gene. 1981 Dec;16(1-3):1–9. doi: 10.1016/0378-1119(81)90055-x. [DOI] [PubMed] [Google Scholar]
- Findley H. W., Jr, Cooper M. D., Kim T. H., Alvarado C., Ragab A. H. Two new acute lymphoblastic leukemia cell lines with early B-cell phenotypes. Blood. 1982 Dec;60(6):1305–1309. [PubMed] [Google Scholar]
- Finger L. R., Huebner K., Cannizzaro L. A., McLeod K., Nowell P. C., Croce C. M. Chromosomal translocation in T-cell leukemia line HUT 78 results in a MYC fusion transcript. Proc Natl Acad Sci U S A. 1988 Dec;85(23):9158–9162. doi: 10.1073/pnas.85.23.9158. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Garson J. A., van den Berghe J. A., Kemshead J. T. Novel non-isotopic in situ hybridization technique detects small (1 Kb) unique sequences in routinely G-banded human chromosomes: fine mapping of N-myc and beta-NGF genes. Nucleic Acids Res. 1987 Jun 25;15(12):4761–4770. doi: 10.1093/nar/15.12.4761. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gilbert F., Balaban G., Moorhead P., Bianchi D., Schlesinger H. Abnormalities of chromosome 1p in human neuroblastoma tumors and cell lines. Cancer Genet Cytogenet. 1982 Sep;7(1):33–42. doi: 10.1016/0165-4608(82)90105-4. [DOI] [PubMed] [Google Scholar]
- Greene M. H., Goldin L. R., Clark W. H., Jr, Lovrien E., Kraemer K. H., Tucker M. A., Elder D. E., Fraser M. C., Rowe S. Familial cutaneous malignant melanoma: autosomal dominant trait possibly linked to the Rh locus. Proc Natl Acad Sci U S A. 1983 Oct;80(19):6071–6075. doi: 10.1073/pnas.80.19.6071. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Haluska F. G., Huebner K., Isobe M., Nishimura T., Croce C. M., Vogt P. K. Localization of the human JUN protooncogene to chromosome region 1p31-32. Proc Natl Acad Sci U S A. 1988 Apr;85(7):2215–2218. doi: 10.1073/pnas.85.7.2215. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hata S., Brenner M. B., Krangel M. S. Identification of putative human T cell receptor delta complementary DNA clones. Science. 1987 Oct 30;238(4827):678–682. doi: 10.1126/science.3499667. [DOI] [PubMed] [Google Scholar]
- Hershfield M. S., Kurtzberg J., Harden E., Moore J. O., Whang-Peng J., Haynes B. F. Conversion of a stem cell leukemia from a T-lymphoid to a myeloid phenotype induced by the adenosine deaminase inhibitor 2'-deoxycoformycin. Proc Natl Acad Sci U S A. 1984 Jan;81(1):253–257. doi: 10.1073/pnas.81.1.253. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Isobe M., Russo G., Haluska F. G., Croce C. M. Cloning of the gene encoding the delta subunit of the human T-cell receptor reveals its physical organization within the alpha-subunit locus and its involvement in chromosome translocations in T-cell malignancy. Proc Natl Acad Sci U S A. 1988 Jun;85(11):3933–3937. doi: 10.1073/pnas.85.11.3933. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kurtzberg J., Bigner S. H., Hershfield M. S. Establishment of the DU.528 human lymphohemopoietic stem cell line. J Exp Med. 1985 Nov 1;162(5):1561–1578. doi: 10.1084/jem.162.5.1561. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kurzrock R., Gutterman J. U., Talpaz M. The molecular genetics of Philadelphia chromosome-positive leukemias. N Engl J Med. 1988 Oct 13;319(15):990–998. doi: 10.1056/NEJM198810133191506. [DOI] [PubMed] [Google Scholar]
- Mathieu-Mahul D., Bernheim A., Sigaux F., Daniel M. T., Larsen C. J., Berger R. Translocation t(1; 14) et remaniement du gène de la chaîne alpha du récepteur T dans une leucémie aiguë lymphoblastique T. C R Acad Sci III. 1986;302(14):525–528. [PubMed] [Google Scholar]
- Münke M., Lindgren V., de Martinville B., Francke U. Comparative analysis of mouse-human hybrids with rearranged chromosomes 1 by in situ hybridization and Southern blotting: high-resolution mapping of NRAS, NGFB, and AMY on human chromosome 1. Somat Cell Mol Genet. 1984 Nov;10(6):589–599. doi: 10.1007/BF01535224. [DOI] [PubMed] [Google Scholar]
- Nau M. M., Brooks B. J., Battey J., Sausville E., Gazdar A. F., Kirsch I. R., McBride O. W., Bertness V., Hollis G. F., Minna J. D. L-myc, a new myc-related gene amplified and expressed in human small cell lung cancer. Nature. 1985 Nov 7;318(6041):69–73. doi: 10.1038/318069a0. [DOI] [PubMed] [Google Scholar]
- Nishikura K., ar-Rushdi A., Erikson J., Watt R., Rovera G., Croce C. M. Differential expression of the normal and of the translocated human c-myc oncogenes in B cells. Proc Natl Acad Sci U S A. 1983 Aug;80(15):4822–4826. doi: 10.1073/pnas.80.15.4822. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rabbitts T. H., Boehm T., Mengle-Gaw L. Chromosomal abnormalities in lymphoid tumours: mechanism and role in tumour pathogenesis. Trends Genet. 1988 Nov;4(11):300–304. doi: 10.1016/0168-9525(88)90106-0. [DOI] [PubMed] [Google Scholar]
- Raimondi S. C., Pui C. H., Behm F. G., Williams D. L. 7q32-q36 translocations in childhood T cell leukemia: cytogenetic evidence for involvement of the T cell receptor beta-chain gene. Blood. 1987 Jan;69(1):131–134. [PubMed] [Google Scholar]
- Sakano H., Kurosawa Y., Weigert M., Tonegawa S. Identification and nucleotide sequence of a diversity DNA segment (D) of immunoglobulin heavy-chain genes. Nature. 1981 Apr 16;290(5807):562–565. doi: 10.1038/290562a0. [DOI] [PubMed] [Google Scholar]
- Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sangster R. N., Minowada J., Suciu-Foca N., Minden M., Mak T. W. Rearrangement and expression of the alpha, beta, and gamma chain T cell receptor genes in human thymic leukemia cells and functional T cells. J Exp Med. 1986 Jun 1;163(6):1491–1508. doi: 10.1084/jem.163.6.1491. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Takihara Y., Tkachuk D., Michalopoulos E., Champagne E., Reimann J., Minden M., Mak T. W. Sequence and organization of the diversity, joining, and constant region genes of the human T-cell delta-chain locus. Proc Natl Acad Sci U S A. 1988 Aug;85(16):6097–6101. doi: 10.1073/pnas.85.16.6097. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tsujimoto Y., Louie E., Bashir M. M., Croce C. M. The reciprocal partners of both the t(14; 18) and the t(11; 14) translocations involved in B-cell neoplasms are rearranged by the same mechanism. Oncogene. 1988 Apr;2(4):347–351. [PubMed] [Google Scholar]
- Valtieri M., Santoli D., Caracciolo D., Kreider B. L., Altmann S. W., Tweardy D. J., Gemperlein I., Mavilio F., Lange B., Rovera G. Establishment and characterization of an undifferentiated human T leukemia cell line which requires granulocyte-macrophage colony stimulatory factor for growth. J Immunol. 1987 Jun 1;138(11):4042–4050. [PubMed] [Google Scholar]
- ar-Rushdi A., Nishikura K., Erikson J., Watt R., Rovera G., Croce C. M. Differential expression of the translocated and the untranslocated c-myc oncogene in Burkitt lymphoma. Science. 1983 Oct 28;222(4622):390–393. doi: 10.1126/science.6414084. [DOI] [PubMed] [Google Scholar]