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. 1989 Jul;86(13):5094–5098. doi: 10.1073/pnas.86.13.5094

Monoclonal antibodies specific to native murine T-cell receptor gamma delta: analysis of gamma delta T cells during thymic ontogeny and in peripheral lymphoid organs.

S Itohara 1, N Nakanishi 1, O Kanagawa 1, R Kubo 1, S Tonegawa 1
PMCID: PMC297563  PMID: 2787028

Abstract

Three hamster monoclonal antibodies (mAbs), all recognizing different epitopes present on the native form of the murine T-cell antigen receptor (TCR) gamma delta subunits, have been generated. mAb 3A10 is specific to a pan-murine TCR gamma delta, recognizing a C delta constant region determinant. mAb 8D6 is specific to a subset of T cells expressing V gamma 4- and V delta 5-encoded gamma delta TCR, and mAb 5C10 is clonotypic. Using these and other mAbs directed against a variety of T-cell surface markers, we quantitated and characterized gamma delta T cells present in developing thymuses as well as in the conventional lymphatic organs by flow cytometry. These studies revealed that (i) many gamma delta thymocytes and peripheral T cells bear CD4 and/or CD8 molecules, (ii) T cells bearing both alpha beta and gamma delta TCRs are scarce, and (iii) thymocyte subsets bearing TCR gamma delta encoded by different combinations of V gamma and V delta gene segments appear in waves during ontogeny.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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