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. 2010 Sep 2;59(10):1331–1339. doi: 10.1136/gut.2009.195370

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© 2010, Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-commercial License, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited, the use is non commercial and is otherwise in compliance with the license. See: http://creativecommons.org/licenses/by-nc/2.0/ and http://creativecommons.org/licenses/by-nc/2.0/legalcode.

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Dietary emulsifier polysorbate-80 increases translocation of E coli across the intestinal epithelium. Polysorbate-80 increased translocation of Crohn's disease E coli across both (A) M-cells and (B) human follicle-associated epithelium (FAE). Similarly, polysorbate-80 enhanced translocation of E coli across (C) parent Caco2-cl1 cells and (D) human villus epithelium (VE). *, p<0.05; **, p<0.01; ANOVA (N=4–8). Crohn's E coli HM605 and EGFP-expressing HM615 were used for the in vitro and ex vivo epithelium translocation studies respectively.

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