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. Author manuscript; available in PMC: 2011 Nov 16.
Published in final edited form as: Biochemistry. 2010 Oct 25;49(45):9882–9890. doi: 10.1021/bi101491u

Figure 1.

Figure 1

RSC catalyzes (A) movement of the DNA relative to the octamer, (B) transfer of H2A/H2B dimers between nucleosomes and (C) transfer of histone octamers between two pieces of DNA. RSC remodeling reactions were run on native gels and scanned in the Cy5 channel. In the reaction schematic shown below each gel, the black circle indicates the position of the Cy5 label and the length of the DNA template is indicated below each substrate. All reactions contained 107 nM RSC, 1 mM ATP and 7 nM Cy5 labeled nucleosome. The reactions shown in B and C contain 28 nM of unlabeled core nucleosomes or free DNA, respectively.