Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1989 Aug;86(16):6153–6156. doi: 10.1073/pnas.86.16.6153

Photoaffinity labeling of indole-3-acetic acid-binding proteins in maize

Alan M Jones *,†, Michael A Venis ‡
PMCID: PMC297795  PMID: 16594060

Abstract

The photoaffinity labeling agent 5-azidoindole-3-acetic acid, an analog of the endogenous plant hormone indole-3-acetic acid (an auxin), was used to identify indole-3-acetic acid-binding proteins in maize. Two peptides with subunit molecular masses of 24 and 22 kilodaltons are specifically labeled in a saturable manner. Both peptides are slightly acidic and behave as dimers under nondenaturing conditions. The possibility that one of these peptides is the auxin receptor that mediates cell elongation in maize is discussed.

Keywords: auxin receptor, 5-azidoindole-3-acetic acid

Full text

PDF
6153

Images in this article

6154Image
on p.6154
6154Image
on p.6154
6155Image
on p.6155
6155Image
on p.6155
6155Image
on p.6155

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barbier-Brygoo H., Ephritikhine G., Klämbt D., Ghislain M., Guern J. Functional evidence for an auxin receptor at the plasmalemma of tobacco mesophyll protoplasts. Proc Natl Acad Sci U S A. 1989 Feb;86(3):891–895. doi: 10.1073/pnas.86.3.891. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brinegar A. C., Cooper G., Stevens A., Hauer C. R., Shabanowitz J., Hunt D. F., Fox J. E. Characterization of a benzyladenine binding-site peptide isolated from a wheat cytokinin-binding protein: sequence analysis and identification of a single affinity-labeled histidine residue by mass spectrometry. Proc Natl Acad Sci U S A. 1988 Aug;85(16):5927–5931. doi: 10.1073/pnas.85.16.5927. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chowdhry V., Westheimer F. H. Photoaffinity labeling of biological systems. Annu Rev Biochem. 1979;48:293–325. doi: 10.1146/annurev.bi.48.070179.001453. [DOI] [PubMed] [Google Scholar]
  4. Hicks G. R., Rayle D. L., Jones A. M., Lomax T. L. Specific photoaffinity labeling of two plasma membrane polypeptides with an azido auxin. Proc Natl Acad Sci U S A. 1989 Jul;86:4948–4952. doi: 10.1073/pnas.86.13.4948. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jones A. M., Melhado L. L., Ho T. H., Leonard N. J. Azido auxins: quantitative binding data in maize. Plant Physiol. 1984 Feb;74(2):295–301. doi: 10.1104/pp.74.2.295. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jones A. M., Melhado L. L., Ho T. H., Pearce C. J., Leonard N. J. Azido auxins : photoaffinity labeling of auxin-binding proteins in maize coleoptile with tritiated 5-azidoindole-3-acetic Acid. Plant Physiol. 1984 Aug;75(4):1111–1116. doi: 10.1104/pp.75.4.1111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  8. Löbler M., Klämbt D. Auxin-binding protein from coleoptile membranes of corn (Zea mays L.). I. Purification by immunological methods and characterization. J Biol Chem. 1985 Aug 15;260(17):9848–9853. [PubMed] [Google Scholar]
  9. Löbler M., Klämbt D. Auxin-binding protein from coleoptile membranes of corn (Zea mays L.). II. Localization of a putative auxin receptor. J Biol Chem. 1985 Aug 15;260(17):9854–9859. [PubMed] [Google Scholar]
  10. Melhado L. L., Jones A. M., Leonard N. J., Vanderhoef L. N. Azido auxins: synthesis and biological activity of fluorescent photoaffinity labeling agents. Plant Physiol. 1981 Aug;68(2):469–475. doi: 10.1104/pp.68.2.469. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  12. Ray P. M. Specificity of Auxin-binding Sites on Maize Coleoptile Membranes as Possible Receptor Sites for Auxin Action. Plant Physiol. 1977 Oct;60(4):585–591. doi: 10.1104/pp.60.4.585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ruoho A. E., Kiefer H., Roeder P. E., Singer S. J. The mechanism of photoaffinity labeling. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2567–2571. doi: 10.1073/pnas.70.9.2567. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Shimomura S., Sotobayashi T., Futai M., Fukui T. Purification and properties of an auxin-binding protein from maize shoot membranes. J Biochem. 1986 May;99(5):1513–1524. doi: 10.1093/oxfordjournals.jbchem.a135621. [DOI] [PubMed] [Google Scholar]
  15. Walton J. D., Ray P. M. Evidence for Receptor Function of Auxin Binding Sites in Maize : RED LIGHT INHIBITION OF MESOCOTYL ELONGATION AND AUXIN BINDING. Plant Physiol. 1981 Dec;68(6):1334–1338. doi: 10.1104/pp.68.6.1334. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES