Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1989 Aug;86(16):6211–6214. doi: 10.1073/pnas.86.16.6211

Chromosome mapping of the human gene encoding the 68-kDa nuclear antigen (p68) by using the polymerase chain reaction.

R Iggo 1, A Gough 1, W Xu 1, D P Lane 1, N K Spurr 1
PMCID: PMC297807  PMID: 2762324

Abstract

Chromosome mapping by Southern analysis of DNA from somatic cell hybrids is often unsuccessful when only cDNA probes are available. p68 is a putative RNA helicase that is antigenically related to the simian virus 40 large tumor antigen. By using the polymerase chain reaction and oligode-oxynucleotide primers based on the cDNA sequence, we have identified introns in the p68 gene. Comparison of human and mouse DNA fragments amplified with these primers revealed length differences that allowed us to identify the human gene. Application of this technique to DNA from human-mouse somatic cell hybrids and cell lines derived from them by chromosome-mediated gene transfer allowed us to map p68 to the distal part of the long arm of chromosome 17.

Full text

PDF
6211

Images in this article

6213Image
on p.6213
6213Image
on p.6213
6214Image
on p.6214

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bai Y., Sheer D., Hiorns L., Knowles R. W., Tunnacliffe A. A monoclonal antibody recognizing a cell surface antigen coded for by a gene on human chromosome 17. Ann Hum Genet. 1982 Oct;46(Pt 4):337–347. doi: 10.1111/j.1469-1809.1982.tb01584.x. [DOI] [PubMed] [Google Scholar]
  2. Ford M. J., Anton I. A., Lane D. P. Nuclear protein with sequence homology to translation initiation factor eIF-4A. Nature. 1988 Apr 21;332(6166):736–738. doi: 10.1038/332736a0. [DOI] [PubMed] [Google Scholar]
  3. Gorbalenya A. E., Koonin E. V., Donchenko A. P., Blinov V. M. A conserved NTP-motif in putative helicases. Nature. 1988 May 5;333(6168):22–22. doi: 10.1038/333022a0. [DOI] [PubMed] [Google Scholar]
  4. Hawkins J. D. A survey on intron and exon lengths. Nucleic Acids Res. 1988 Nov 11;16(21):9893–9908. doi: 10.1093/nar/16.21.9893. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hodgman T. C. A new superfamily of replicative proteins. Nature. 1988 May 5;333(6168):22–23. doi: 10.1038/333022b0. [DOI] [PubMed] [Google Scholar]
  6. Lane D. P., Hoeffler W. K. SV40 large T shares an antigenic determinant with a cellular protein of molecular weight 68,000. Nature. 1980 Nov 13;288(5787):167–170. doi: 10.1038/288167a0. [DOI] [PubMed] [Google Scholar]
  7. Lane D. Enlarged family of putative helicases. Nature. 1988 Aug 11;334(6182):478–478. doi: 10.1038/334478a0. [DOI] [PubMed] [Google Scholar]
  8. Lasko P. F., Ashburner M. The product of the Drosophila gene vasa is very similar to eukaryotic initiation factor-4A. Nature. 1988 Oct 13;335(6191):611–617. doi: 10.1038/335611a0. [DOI] [PubMed] [Google Scholar]
  9. Martin D., Tucker D. F., Gorman P., Sheer D., Spurr N. K., Trowsdale J. The human placental alkaline phosphatase gene and related sequences map to chromosome 2 band q37. Ann Hum Genet. 1987 May;51(Pt 2):145–152. doi: 10.1111/j.1469-1809.1987.tb01056.x. [DOI] [PubMed] [Google Scholar]
  10. Nielsen P. J., Trachsel H. The mouse protein synthesis initiation factor 4A gene family includes two related functional genes which are differentially expressed. EMBO J. 1988 Jul;7(7):2097–2105. doi: 10.1002/j.1460-2075.1988.tb03049.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nishi K., Morel-Deville F., Hershey J. W., Leighton T., Schnier J. An eIF-4A-like protein is a suppressor of an Escherichia coli mutant defective in 50S ribosomal subunit assembly. Nature. 1988 Dec 1;336(6198):496–498. doi: 10.1038/336496a0. [DOI] [PubMed] [Google Scholar]
  12. Povey S., Jeremiah S. J., Barker R. F., Hopkinson D. A., Robson E. B., Cook P. J., Solomon E., Bobrow M., Carritt B., Buckton K. E. Assignment of the human locus determining phosphoglycolate phosphatase (PGP) to chromosome 16. Ann Hum Genet. 1980 Jan;43(3):241–248. doi: 10.1111/j.1469-1809.1980.tb01557.x. [DOI] [PubMed] [Google Scholar]
  13. Saiki R. K., Scharf S., Faloona F., Mullis K. B., Horn G. T., Erlich H. A., Arnheim N. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science. 1985 Dec 20;230(4732):1350–1354. doi: 10.1126/science.2999980. [DOI] [PubMed] [Google Scholar]
  14. Solomon E., Bobrow M., Goodfellow P. N., Bodmer W. F., Swallow D. M., Povey S., Noël B. Human gene mapping using an X/autosome translocation. Somatic Cell Genet. 1976 Mar;2(2):125–140. doi: 10.1007/BF01542626. [DOI] [PubMed] [Google Scholar]
  15. Solomon E., Swallow D., Burgess S., Evans L. Assignment of the human acid alpha-glucosidase gene (alphaGLU) to chromosome 17 using somatic cell hybrids. Ann Hum Genet. 1979 Jan;42(3):273–281. doi: 10.1111/j.1469-1809.1979.tb00661.x. [DOI] [PubMed] [Google Scholar]
  16. Spurr N. K., Durbin H., Sheer D., Parkar M., Bobrow L., Bodmer W. F. Characterization and chromosomal assignment of a human cell surface antigen defined by the monoclonal antibody AUAI. Int J Cancer. 1986 Nov 15;38(5):631–636. doi: 10.1002/ijc.2910380503. [DOI] [PubMed] [Google Scholar]
  17. Swallow D. M., Solomon E., Pajunen L. Immunochemical analysis of the N-acetyl hexosaminidases in human-mouse hybrids made using a double selective system. Cytogenet Cell Genet. 1977;18(3):136–148. doi: 10.1159/000130758. [DOI] [PubMed] [Google Scholar]
  18. Séraphin B., Simon M., Boulet A., Faye G. Mitochondrial splicing requires a protein from a novel helicase family. Nature. 1989 Jan 5;337(6202):84–87. doi: 10.1038/337084a0. [DOI] [PubMed] [Google Scholar]
  19. Voss R., Lerer I., Povey S., Solomon E., Bobrow M. Confirmation and further regional assignment of aminoacylase 1 (acy-1) on human chromosome 3 using a simplified detection method. Ann Hum Genet. 1980 Jul;44(Pt 1):1–9. doi: 10.1111/j.1469-1809.1980.tb00940.x. [DOI] [PubMed] [Google Scholar]
  20. Xu W. M., Gorman P. A., Rider S. H., Hedge P. J., Moore G., Prichard C., Sheer D., Solomon E. Construction of a genetic map of human chromosome 17 by use of chromosome-mediated gene transfer. Proc Natl Acad Sci U S A. 1988 Nov;85(22):8563–8567. doi: 10.1073/pnas.85.22.8563. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. van Heyningen V., Bobrow M., Bodmer W. F., Gardiner S. E., Povey S., Hopkinson D. A. Chromosome assignment of some human enzyme loci: mitochondrial malate dehydrogenase to 7, mannosephosphate isomerase and pyruvate kinase to 15 and probably, esterase D to 13. Ann Hum Genet. 1975 Jan;38(3):295–303. doi: 10.1111/j.1469-1809.1975.tb00613.x. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES