Fig. 5.

In vivo effects of LDE on the expressions of tumor VEGF, HGF, and OPN proteins in melanoma-bearing mice. After the mice in the control group (n = 8) and LDE group (n = 8) were killed, each tumor in both the groups were excised. The expressions of VEGF, HGF, and OPN proteins was in each tumor were analyzed by ELISA as described in “Materials and methods” section. Each point and vertical bar represents the mean and SD for six wells. The figures (a, b, and c) are the representatives of three similar experiments performed. Statistical analysis was carried out using the ANOVA and Bonferroni test. All statistical tests were two-sided. ** P < 0.01, *** P < 0.001