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. 1989 Sep;86(17):6459–6463. doi: 10.1073/pnas.86.17.6459

Multistep pathway for replication-dependent nucleosome assembly.

R Fotedar 1, J M Roberts 1
PMCID: PMC297863  PMID: 2771936

Abstract

We have used cell-free DNA replication to study the relationship between DNA replication and chromatin assembly. As others have reported, we find that DNA replication facilitates nucleosome assembly. We show here that replication-dependent nucleosome assembly occurs in at least two steps. The first step requires replicating DNA; the second step occurs after replication has been completed and is promoted by a nuclear extract. Consistent with this multistep model, we observe that the replicated simian virus 40 minichromosome is organized into a repeating array of DNA-protein particles that are structurally distinct from mature nucleosomes. These particles may be precursors in a pathway of nucleosome assembly since in the second, replication-independent step the nuclear extract converts this nascent chromatin into nucleosomes.

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Selected References

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