Figure 2.

B-Raf kinase activation induces cell surface HS degradation. (A) HEK293 cells transiently transfected with the indicated vectors. After incubating for 48 hours, the cells were harvested and analyzed for cell surface HS levels by FACS. (B) HPR1 inhibitors restored cell surface HS levels. Cells transiently transfected with the empty vector or the vector encoding BRAF^V600E or HRAS^G12V in the absence or presence of PI-88 or heparin (50 µg/ml) for 48 hours were analyzed for cell surface HS levels by FACS with anti-HSmAb. Green line indicates IgM control; red line, anti-HS mAb.