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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1989 Sep;86(18):6925–6929. doi: 10.1073/pnas.86.18.6925

Human immunodeficiency virus 1 tat protein binds trans-activation-responsive region (TAR) RNA in vitro.

C Dingwall 1, I Ernberg 1, M J Gait 1, S M Green 1, S Heaphy 1, J Karn 1, A D Lowe 1, M Singh 1, M A Skinner 1, R Valerio 1
PMCID: PMC297963  PMID: 2476805

Abstract

tat, the trans-activator protein for human immunodeficiency virus 1 (HIV-1), has been expressed in Escherichia coli from synthetic genes. Purified tat binds specifically to HIV-1 trans-activation-responsive region (TAR) RNA in gel-retardation, filter-binding, and immunoprecipitation assays. tat does not bind detectably to antisense TAR RNA sequences, cellular mRNA sequences, variant TAR RNA sequences with altered stem-loop structures, or TAR DNA.

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Selected References

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