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. 1989 Oct;86(19):7571–7574. doi: 10.1073/pnas.86.19.7571

Immunoregulatory activity of peptides related to platelet factor 4.

M B Zucker 1, I R Katz 1, G J Thorbecke 1, D C Milot 1, J Holt 1
PMCID: PMC298107  PMID: 2678107

Abstract

Platelet factor 4 (PF4), a secreted platelet protein, alleviates concanavalin A-induced immunosuppression in mice. We now find that activity also resides in (i) the C-terminal tridecapeptide of PF4 (P13S), (ii) an analog of this in which arginine replaces the lysine residues and in which the last two amino acids are absent, (iii) the C-terminal 18 amino acids of low-affinity platelet factor 4, which is very similar to P13S, and (iv) peptide fragments of P13S that contain only 5-9 amino acids. P13S treated with fluorescamine to derivatize the free amino groups retained immunoregulatory activity but did not bind to heparin-agarose. The N-terminal and middle portions of PF4, polylysine, protamine, and three unrelated peptides were inactive in this assay.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barone A. D., Ghrayeb J., Hammerling U., Zucker M. B., Thorbecke G. J. The expression in Escherichia coli of recombinant human platelet factor 4, a protein with immunoregulatory activity. J Biol Chem. 1988 Jun 25;263(18):8710–8715. [PubMed] [Google Scholar]
  2. Böhlen P., Stein S., Dairman W., Udenfriend S. Fluorometric assay of proteins in the nanogram range. Arch Biochem Biophys. 1973 Mar;155(1):213–220. doi: 10.1016/s0003-9861(73)80023-2. [DOI] [PubMed] [Google Scholar]
  3. Deuel T. F., Senior R. M., Chang D., Griffin G. L., Heinrikson R. L., Kaiser E. T. Platelet factor 4 is chemotactic for neutrophils and monocytes. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4584–4587. doi: 10.1073/pnas.78.7.4584. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hewick R. M., Hunkapiller M. W., Hood L. E., Dreyer W. J. A gas-liquid solid phase peptide and protein sequenator. J Biol Chem. 1981 Aug 10;256(15):7990–7997. [PubMed] [Google Scholar]
  5. Holt J. C., Niewiarowski S. Biochemistry of alpha granule proteins. Semin Hematol. 1985 Apr;22(2):151–163. [PubMed] [Google Scholar]
  6. Holt J. C., Niewiarowski S. Platelet basic protein, low-affinity platelet factor 4, and beta-thromboglobulin: purification and identification. Methods Enzymol. 1989;169:224–233. doi: 10.1016/0076-6879(89)69063-5. [DOI] [PubMed] [Google Scholar]
  7. JERNE N. K., NORDIN A. A. Plaque formation in agar by single antibody-producing cells. Science. 1963 Apr 26;140(3565):405–405. [PubMed] [Google Scholar]
  8. Katz I. R., Bell M. K., Hoffman M. K., Thorbecke G. J. Reversal of Con A-induced suppression by a platelet-derived factor which binds to activated suppressor T cells. Cell Immunol. 1986 Jun;100(1):57–65. doi: 10.1016/0008-8749(86)90006-7. [DOI] [PubMed] [Google Scholar]
  9. Katz I. R., Hoffmann M. K., Zucker M. B., Bell M. K., Thorbecke G. J. A platelet-derived immunoregulatory serum factor with T cell affinity. J Immunol. 1985 May;134(5):3199–3203. [PubMed] [Google Scholar]
  10. Katz I. R., Thorbecke G. J., Bell M. K., Yin J. Z., Clarke D., Zucker M. B. Protease-induced immunoregulatory activity of platelet factor 4. Proc Natl Acad Sci U S A. 1986 May;83(10):3491–3495. doi: 10.1073/pnas.83.10.3491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lawler J. W. Prediction of the secondary structure of platelet factor 4 and beta-thromboglobulin from their amino acid sequences. Thromb Res. 1981 Jan 1;21(1-2):121–127. doi: 10.1016/0049-3848(84)90039-2. [DOI] [PubMed] [Google Scholar]
  12. Malinconico S. M., Katz J. B., Budzynski A. Z. Fibrinogen degradation by hementin, a fibrinogenolytic anticoagulant from the salivary glands of the leech Haementeria ghilianii. J Lab Clin Med. 1984 Nov;104(5):842–854. [PubMed] [Google Scholar]
  13. Mishell R. I., Dutton R. W. Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967 Sep 1;126(3):423–442. doi: 10.1084/jem.126.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Osterman D. G., Griffin G. L., Senior R. M., Kaiser E. T., Deuel T. F. The carboxyl-terminal tridecapeptide of platelet factor 4 is a potent chemotactic agent for monocytes. Biochem Biophys Res Commun. 1982 Jul 16;107(1):130–135. doi: 10.1016/0006-291x(82)91679-5. [DOI] [PubMed] [Google Scholar]
  15. Smith A. L., Bottomly K., Winograd D. F. Altered splenic T cell function of BALB/cByJ mice infected with mouse hepatitis virus or Sendai virus. J Immunol. 1987 May 15;138(10):3426–3430. [PubMed] [Google Scholar]
  16. St Charles R., Walz D. A., Edwards B. F. The three-dimensional structure of bovine platelet factor 4 at 3.0-A resolution. J Biol Chem. 1989 Feb 5;264(4):2092–2099. [PubMed] [Google Scholar]

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