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. 1989 Oct;86(20):7805–7808. doi: 10.1073/pnas.86.20.7805

A member of the Hsp70 family is localized in mitochondria and resembles Escherichia coli DnaK.

T Leustek 1, B Dalie 1, D Amir-Shapira 1, N Brot 1, H Weissbach 1
PMCID: PMC298159  PMID: 2682628

Abstract

A 71-kDa protein (P71) with properties similar to those of the Escherichia coli heat shock protein DnaK has been found in extracts of HeLa cells. P71 was copurified by ATP-agarose affinity chromatography with three additional proteins of the Hsp70 family. Of these proteins, only P71 crossreacted strongly with antiserum raised against purified DnaK, and both DnaK and P71 could be phosphorylated in vitro with [gamma-32]ATP in a reaction that was markedly stimulated by Ca2+. In HeLa cells, P71 was found to be concentrated in mitochondria. A protein similar to P71 was also found in calf liver and yeast mitochondria.

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