Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1989 Oct;86(20):8029–8033. doi: 10.1073/pnas.86.20.8029

Two high-affinity interleukin 1 receptors represent separate gene products.

R Chizzonite 1, T Truitt 1, P L Kilian 1, A S Stern 1, P Nunes 1, K P Parker 1, K L Kaffka 1, A O Chua 1, D K Lugg 1, U Gubler 1
PMCID: PMC298207  PMID: 2530579

Abstract

Interleukin 1 (IL-1) is a polypeptide hormone that mediates a broad range of biological activities and interacts with surface receptors on numerous cell types. Equilibrium binding studies have identified a class of IL-1 receptors on T cells, fibroblasts, and epithelial cells that have 2- to 5-fold higher affinity than the receptors on bone marrow cells, pre-B cells, and macrophage cell lines. Affinity cross-linking with human 125I-labeled IL-1 alpha (125I-IL-1 alpha) labels an approximately 100-kDa protein on T cells and fibroblasts and an approximately 80-kDa protein on pre-B cells and macrophage cell lines. Monoclonal and polyclonal antibodies specific for the IL-1 receptor on T cells and fibroblasts block human 125I-IL-1 alpha binding to T cells, fibroblasts, and epithelial cells but cannot block IL-1 binding to bone marrow cells, pre-B cells, and macrophages. These antibodies immunoprecipitate the IL-1 receptor-human 125I-IL-1 alpha complex from T cells and fibroblasts but not from pre-B cells and macrophage cell lines. An S1 nuclease protection assay demonstrated that T cells and fibroblasts contain identical IL-1 receptor mRNA but that pre-B cells and macrophages do not contain this receptor mRNA. Taken together, the data demonstrate that mouse T cells, fibroblasts, and epithelial cells express an identical IL-1 receptor, whereas the IL-1 receptor on pre-B cells, macrophages, and bone marrow cells represents a different gene product.

Full text

PDF
8029

Images in this article

8031Image
on p.8031
8031Image
on p.8031
8031Image
on p.8031
8031Image
on p.8031
8031Image
on p.8031
8032Image
on p.8032

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bauer J., Weber W., Tran-Thi T. A., Northoff G. H., Decker K., Gerok W., Heinrich P. C. Murine interleukin 1 stimulates alpha 2-macroglobulin synthesis in rat hepatocyte primary cultures. FEBS Lett. 1985 Oct 14;190(2):271–274. doi: 10.1016/0014-5793(85)81298-9. [DOI] [PubMed] [Google Scholar]
  2. Benjamin W. R., Tare N. S., Hayes T. J., Becker J. M., Anderson T. D. Regulation of hemopoiesis in myelosuppressed mice by human recombinant IL-1 alpha. J Immunol. 1989 Feb 1;142(3):792–799. [PubMed] [Google Scholar]
  3. Bird T. A., Gearing A. J., Saklatvala J. Murine interleukin 1 receptor. Direct identification by ligand blotting and purification to homogeneity of an interleukin 1-binding glycoprotein. J Biol Chem. 1988 Aug 25;263(24):12063–12069. [PubMed] [Google Scholar]
  4. Bird T. A., Gearing A. J., Saklatvala J. Murine interleukin-1 receptor: differences in binding properties between fibroblastic and thymoma cells and evidence for a two-chain receptor model. FEBS Lett. 1987 Dec 10;225(1-2):21–26. doi: 10.1016/0014-5793(87)81124-9. [DOI] [PubMed] [Google Scholar]
  5. Bird T. A., Saklatvala J. Identification of a common class of high affinity receptors for both types of porcine interleukin-1 on connective tissue cells. Nature. 1986 Nov 20;324(6094):263–266. doi: 10.1038/324263a0. [DOI] [PubMed] [Google Scholar]
  6. Chin J., Cameron P. M., Rupp E., Schmidt J. A. Identification of a high-affinity receptor for native human interleukin 1 beta and interleukin 1 alpha on normal human lung fibroblasts. J Exp Med. 1987 Jan 1;165(1):70–86. doi: 10.1084/jem.165.1.70. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dower S. K., Call S. M., Gillis S., Urdal D. L. Similarity between the interleukin 1 receptors on a murine T-lymphoma cell line and on a murine fibroblast cell line. Proc Natl Acad Sci U S A. 1986 Feb;83(4):1060–1064. doi: 10.1073/pnas.83.4.1060. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dower S. K., Kronheim S. R., Hopp T. P., Cantrell M., Deeley M., Gillis S., Henney C. S., Urdal D. L. The cell surface receptors for interleukin-1 alpha and interleukin-1 beta are identical. Nature. 1986 Nov 20;324(6094):266–268. doi: 10.1038/324266a0. [DOI] [PubMed] [Google Scholar]
  9. Dower S. K., Kronheim S. R., March C. J., Conlon P. J., Hopp T. P., Gillis S., Urdal D. L. Detection and characterization of high affinity plasma membrane receptors for human interleukin 1. J Exp Med. 1985 Aug 1;162(2):501–515. doi: 10.1084/jem.162.2.501. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Dukovich M., Severin J. M., White S. J., Yamazaki S., Mizel S. B. Stimulation of fibroblast proliferation and prostaglandin production by purified recombinant murine interleukin 1. Clin Immunol Immunopathol. 1986 Mar;38(3):381–389. doi: 10.1016/0090-1229(86)90248-5. [DOI] [PubMed] [Google Scholar]
  11. Gubler U., Chua A. O., Stern A. S., Hellmann C. P., Vitek M. P., DeChiara T. M., Benjamin W. R., Collier K. J., Dukovich M., Familletti P. C. Recombinant human interleukin 1 alpha: purification and biological characterization. J Immunol. 1986 Apr 1;136(7):2492–2497. [PubMed] [Google Scholar]
  12. Gubler U., Chua A. O., Young D., Fan Z. W., Eng J. Cholecystokinin mRNA in porcine cerebellum. J Biol Chem. 1987 Nov 5;262(31):15242–15245. [PubMed] [Google Scholar]
  13. Howard M., Nakanishi K., Paul W. E. B cell growth and differentiation factors. Immunol Rev. 1984 Apr;78:185–210. doi: 10.1111/j.1600-065x.1984.tb00482.x. [DOI] [PubMed] [Google Scholar]
  14. Katz M. E., Tite J. P., Janeway C. A., Jr The immunobiology of T cell responses to Mls-locus-disparate stimulator cells. III. Helper and cytolytic functions of cloned, Mls-reactive T cell lines. J Immunol. 1986 Jan;136(1):1–5. [PubMed] [Google Scholar]
  15. Kaye J., Porcelli S., Tite J., Jones B., Janeway C. A., Jr Both a monoclonal antibody and antisera specific for determinants unique to individual cloned helper T cell lines can substitute for antigen and antigen-presenting cells in the activation of T cells. J Exp Med. 1983 Sep 1;158(3):836–856. doi: 10.1084/jem.158.3.836. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  17. Lowenthal J. W., MacDonald H. R. Binding and internalization of interleukin 1 by T cells. Direct evidence for high- and low-affinity classes of interleukin 1 receptor. J Exp Med. 1986 Oct 1;164(4):1060–1074. doi: 10.1084/jem.164.4.1060. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Matsushima K., Akahoshi T., Yamada M., Furutani Y., Oppenheim J. J. Properties of a specific interleukin 1 (IL 1) receptor on human Epstein Barr virus-transformed B lymphocytes: identity of the receptor for IL 1-alpha and IL 1-beta. J Immunol. 1986 Jun 15;136(12):4496–4502. [PubMed] [Google Scholar]
  19. Matsushima K., Yodoi J., Tagaya Y., Oppenheim J. J. Down-regulation of interleukin 1 (IL 1) receptor expression by IL 1 and fate of internalized 125I-labeled IL 1 beta in a human large granular lymphocyte cell line. J Immunol. 1986 Nov 15;137(10):3183–3188. [PubMed] [Google Scholar]
  20. Mizel S. B., Dayer J. M., Krane S. M., Mergenhagen S. E. Stimulation of rheumatoid synovial cell collagenase and prostaglandin production by partially purified lymphocyte-activating factor (interleukin 1). Proc Natl Acad Sci U S A. 1981 Apr;78(4):2474–2477. doi: 10.1073/pnas.78.4.2474. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Mizel S. B., Kilian P. L., Lewis J. C., Paganelli K. A., Chizzonite R. A. The interleukin 1 receptor. Dynamics of interleukin 1 binding and internalization in T cells and fibroblasts. J Immunol. 1987 May 1;138(9):2906–2912. [PubMed] [Google Scholar]
  22. Mizel S. B., Oppenheim J. J., Rosentreich D. L. Characterization of lymphocyte-activating factor (LAF) produced by a macrophage cell line, P388D1. II. Biochemical characterization of LAF induced by activated T cells and LPS. J Immunol. 1978 May;120(5):1504–1508. [PubMed] [Google Scholar]
  23. Mochizuki D. Y., Eisenman J. R., Conlon P. J., Larsen A. D., Tushinski R. J. Interleukin 1 regulates hematopoietic activity, a role previously ascribed to hemopoietin 1. Proc Natl Acad Sci U S A. 1987 Aug;84(15):5267–5271. doi: 10.1073/pnas.84.15.5267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Mohagheghpour N., Damle N. K., Moonka D. K., Terrell C. P., Engleman E. G. A human alloreactive inducer T cell clone that selectively activates antigen-specific suppressor T cells. J Immunol. 1984 Jul;133(1):133–136. [PubMed] [Google Scholar]
  25. Paganelli K. A., Stern A. S., Kilian P. L. Detergent solubilization of the interleukin 1 receptor. J Immunol. 1987 Apr 1;138(7):2249–2253. [PubMed] [Google Scholar]
  26. Parker K. P., Benjamin W. R., Kaffka K. L., Kilian P. L. Presence of IL-1 receptors on human and murine neutrophils. Relevance to IL-1-mediated effects in inflammation. J Immunol. 1989 Jan 15;142(2):537–542. [PubMed] [Google Scholar]
  27. Perlmutter D. H., Goldberger G., Dinarello C. A., Mizel S. B., Colten H. R. Regulation of class III major histocompatibility complex gene products by interleukin-1. Science. 1986 May 16;232(4752):850–852. doi: 10.1126/science.3010455. [DOI] [PubMed] [Google Scholar]
  28. Ramadori G., Sipe J. D., Dinarello C. A., Mizel S. B., Colten H. R. Pretranslational modulation of acute phase hepatic protein synthesis by murine recombinant interleukin 1 (IL-1) and purified human IL-1. J Exp Med. 1985 Sep 1;162(3):930–942. doi: 10.1084/jem.162.3.930. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Schmidt J. A., Mizel S. B., Cohen D., Green I. Interleukin 1, a potential regulator of fibroblast proliferation. J Immunol. 1982 May;128(5):2177–2182. [PubMed] [Google Scholar]
  30. Sims J. E., March C. J., Cosman D., Widmer M. B., MacDonald H. R., McMahan C. J., Grubin C. E., Wignall J. M., Jackson J. L., Call S. M. cDNA expression cloning of the IL-1 receptor, a member of the immunoglobulin superfamily. Science. 1988 Jul 29;241(4865):585–589. doi: 10.1126/science.2969618. [DOI] [PubMed] [Google Scholar]
  31. Uhl J., Newton R. C., Giri J. G., Sandlin G., Horuk R. Identification of IL-1 receptors on human monocytes. J Immunol. 1989 Mar 1;142(5):1576–1581. [PubMed] [Google Scholar]
  32. Urdal D. L., Call S. M., Jackson J. L., Dower S. K. Affinity purification and chemical analysis of the interleukin-1 receptor. J Biol Chem. 1988 Feb 25;263(6):2870–2877. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES